High-throughput RNAi screening in cultured cells: a user's guide

Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans

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Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells

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A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays.

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The nuclear RNase III Drosha initiates microRNA processing

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A Species of Small Antisense RNA in Posttranscriptional Gene Silencing in Plants

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Processing of primary microRNAs by the Microprocessor complex

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The Microprocessor complex mediates the genesis of microRNAs

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Nuclear Export of MicroRNA Precursors

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Expression profiling reveals off-target gene regulation by RNAi

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TRBP recruits the Dicer complex to Ago2 for microRNA processing and gene silencing

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Rational siRNA design for RNA interference

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Functional genomic analysis of C. elegans chromosome I by systematic RNA interference

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Exportin 5 is a RanGTP-dependent dsRNA-binding protein that mediates nuclear export of pre-miRNAs

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Inhibition of Translational Initiation by Let-7 MicroRNA in Human Cells

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Activation of the interferon system by short-interfering RNAs

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Regulation by let-7 and lin-4 miRNAs Results in Target mRNA Degradation

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siRNAs can function as miRNAsPreprint

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Cell lines derived from late embryonic stages of Drosophila melanogaster

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A large-scale RNAi screen in human cells identifies new components of the p53 pathway

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The human DiGeorge syndrome critical region gene 8 and Its D. melanogaster homolog are required for miRNA biogenesis.

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Microarrays of cells expressing defined cDNAs

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3′ UTR seed matches, but not overall identity, are associated with RNAi off-targets

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Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans

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Synthetic dsRNA Dicer substrates enhance RNAi potency and efficacy

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Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III

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Use of double-stranded RNA interference in <i>Drosophila</i> cell lines to dissect signal transduction pathways

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Genome-Wide RNAi Analysis of Growth and Viability in Drosophila Cells

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A resource for large-scale RNA-interference-based screens in mammals

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Second-generation shRNA libraries covering the mouse and human genomes

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Short interfering RNAs can induce unexpected and divergent changes in the levels of untargeted proteins in mammalian cells

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Genome-wide analysis of human kinases in clathrin- and caveolae/raft-mediated endocytosis

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A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells.

Stegmeier, F., Hu, G., Rickles, R., Hannon, G., & Elledge, S. (2005) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.0506306102 · Cited by 584 · see citing works

Sensitized RNAi screen of human kinases and phosphatases identifies new regulators of apoptosis and chemoresistance

MacKeigan, J. P., Murphy, L. O., & Blenis, J. (2005) · Nature Cell Biology · doi:10.1038/ncb1258 · Cited by 561 · see citing works

Probing tumor phenotypes using stable and regulated synthetic microRNA precursors

Dickins, R., Hemann, M., Zilfou, J. T., Simpson, D. R., Ibarra, I., Hannon, G., & Lowe, S. (2005) · Nature Genetics · doi:10.1038/ng1651 · Cited by 561 · see citing works

Normal microRNA Maturation and Germ-Line Stem Cell Maintenance Requires Loquacious, a Double-Stranded RNA-Binding Domain Protein

Förstemann, K., Tomari, Y., Du, T., Vagin, V. V., Denli, A. M., Bratu, D. P., Klattenhoff, C., Theurkauf, W. E., & Zamore, P. D. (2005) · PLoS Biology · doi:10.1371/journal.pbio.0030236 · Cited by 545 · see citing works

Identification of Hedgehog Pathway Components by RNAi in Drosophila Cultured Cells

Lum, L., Yao, S., Mozer, B. A., Rovescalli, A. C., von Kessler, D. P., Nirenberg, M. W., & Beachy, P. A. (2003) · Science · doi:10.1126/science.1081403 · Cited by 532 · see citing works

A Genetic Screen for Candidate Tumor Suppressors Identifies REST

Westbrook, T. F., Martin, E. S., Schlabach, M. R., Leng, Y., Liang, A. C. T., Feng, B., Zhao, J. J., Roberts, T. M., Mandel, G., Hannon, G. J., et al. (2005) · Cell · doi:10.1016/j.cell.2005.03.033 · Cited by 487 · see citing works

Analysis of Clathrin-mediated Endocytosis of Epidermal Growth Factor Receptor by RNA Interference

Huang, F., Khvorova, A., Marshall, W., & Sorkin, A. D. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.c400046200 · Cited by 460 · see citing works

Processing of Pre-microRNAs by the Dicer-1–Loquacious Complex in Drosophila Cells

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A functional genomic analysis of cell morphology using RNA interference

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An endoribonuclease-prepared siRNA screen in human cells identifies genes essential for cell division

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In vitrol culture ofDrosophila melanogaster embryonic cells

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Design of a genome-wide siRNA library using an artificial neural network

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Drosophila RNAi Screen Reveals CD36 Family Member Required for Mycobacterial Infection

Philips, J. A., Rubin, E. J., & Perrimon, N. (2005) · Science · doi:10.1126/science.1116006 · Cited by 370 · see citing works

Activation of the mammalian immune system by siRNAs

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Genome-wide survey of protein kinases required for cell cycle progression

Bettencourt‐Dias, M., Giet, R., Sinka, R., Mazumdar, A., Lock, W. G., Balloux, F., Zafiropoulos, P. J., Yamaguchi, S., Winter, S. L., Carthew, R. W., et al. (2004) · Nature · doi:10.1038/nature03160 · Cited by 345 · see citing works

Functional Genomic Analysis of the Wnt-Wingless Signaling Pathway

DasGupta, R., Kaykas, A., Moon, R. T., & Perrimon, N. (2005) · Science · doi:10.1126/science.1109374 · Cited by 331 · see citing works

Parallel Chemical Genetic and Genome-Wide RNAi Screens Identify Cytokinesis Inhibitors and Targets

Eggert, U., Kiger, A. A., Richter, C., Perlman, Z. E., Perrimon, N., Mitchison, T. J., & Field, C. M. (2004) · PLoS Biology · doi:10.1371/journal.pbio.0020379 · Cited by 325 · see citing works

Short RNA duplexes produced by hydrolysis with Escherichia coli RNase III mediate effective RNA interference in mammalian cells

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Genome-Wide RNAi Screen for Host Factors Required for Intracellular Bacterial Infection

Agaisse, H., Burrack, L. S., Philips, J. A., Rubin, E. J., Perrimon, N., & Higgins, D. E. (2005) · Science · doi:10.1126/science.1116008 · Cited by 308 · see citing works

Identification of JAK/STAT signalling components by genome-wide RNA interference

Müller, P., Kuttenkeuler, D., Gesellchen, V., Zeidler, M. P., & Boutros, M. (2005) · Nature · doi:10.1038/nature03869 · Cited by 297 · see citing works

Systematic genome-wide screens of gene function

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A Genetic Screen Identifies PITX1 as a Suppressor of RAS Activity and Tumorigenicity

Kolfschoten, I., van Leeuwen, B., Berns, K., Mullenders, J., Beijersbergen, R. L., Bernards, R., Voorhoeve, P. M., & Agami, R. (2005) · Cell · doi:10.1016/j.cell.2005.04.017 · Cited by 273 · see citing works

Functional Dissection of an Innate Immune Response by a Genome-Wide RNAi Screen

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Sustained Small Interfering RNA-Mediated HumanImmunodeficiency Virus Type 1 Inhibition in PrimaryMacrophages

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Genome-wide RNAi screen reveals a specific sensitivity of IRES-containing RNA viruses to host translation inhibitionPreprint

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Genome-wide RNAi analysis of JAK/STAT signaling components in DrosophilaPreprint

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Small Interfering RNA-Mediated Gene Silencing in T Lymphocytes

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The ferredoxin reductase gene is regulated by the p53 family and sensitizes cells to oxidative stress-induced apoptosis

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RNAi Microarray Analysis in Cultured Mammalian CellsPreprint

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A genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway

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Identification and Characterization of a Novel Line of Drosophila Schneider S2 Cells That Respond to Wingless Signaling

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Functional genomics to new drug targets

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RNA interference microarrays: High-throughput loss-of-function genetics in mammalian cells

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An RNA interference screen ide.jpgies Inhibitor of Apoptosis Protein 2 as a regulator of innate immune signalling in Drosophila

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RNAi living-cell microarrays for loss-of-function screens in Drosophila melanogaster cells

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Microarrays of lentiviruses for gene function screens in immortalized and primary cells

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High-throughput RNAi screening in vitro: From cell lines to primary cells

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A TRAIL receptor-dependent synthetic lethal relationship between MYC activation and GSK3β/FBW7 loss of function

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Involvement of MINK, a Ste20 Family Kinase, in Ras Oncogene-Induced Growth Arrest in Human Ovarian Surface Epithelial Cells

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In situ fluorescence analysis demonstrates active siRNA exclusion from the nucleus by Exportin 5

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Newly established cell lines fromDrosophila larval CNS express neural specific characteristics

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RNA interference rescue by bacterial artificial chromosome transgenesis in mammalian tissue culture cells

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High-Throughput RNA Interference Screens in Drosophila Tissue Culture Cells

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siRNA Cell Arrays for High-Content Screening Microscopy

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Genes required for Drosophila nervous system development identified by RNA interference

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The ultrastructure of imaginal disc cells in primary cultures and during cell aggregation in continuous cell lines

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Drosophila Cell Culture

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CellProfiler: image analysis software for identifying and quantifying cell phenotypes

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Genome-scale CRISPR-Cas9 Knockout and Transcriptional Activation Screening

Joung, J., Konermann, S., Gootenberg, J. S., Abudayyeh, O. O., Platt, R. J., Brigham, M. D., Sanjana, N. E., & Zhang, F. (2017) · Nature Protocols · doi:10.1038/nprot.2017.016 · Cited by 1,243 · see citing works

High-throughput functional genomics using CRISPR-Cas9

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The Novel Tail-anchored Membrane Protein Mff Controls Mitochondrial and Peroxisomal Fission in Mammalian Cells

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Molecular Basis of Calcium Signaling in Lymphocytes: STIM and ORAI

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Exploring genetic interactions and networks with yeast

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Dynamic Proteomics of Individual Cancer Cells in Response to a Drug

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The art and design of genetic screens: RNA interference

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The growing catalog of small RNAs and their association with distinct Argonaute/Piwi family members

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Minimizing the risk of reporting false positives in large-scale RNAi screens

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Identification of genes that regulate epithelial cell migration using an siRNA screening approach

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Bioimage informatics: a new area of engineering biology

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Genetics on the Fly: A Primer on the Drosophila Model System

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Signalling to transcription: Store-operated Ca2+ entry and NFAT activation in lymphocytes

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Genome-scale RNAi profiling of cell division in human tissue culture cells

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