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Cited by 454

The DOI matches its Crossref record; 90 of the 90 references it cites were found in a registry or an index.

Adaptation of High-Throughput Screening in Drug Discovery—Toxicological Screening Tests

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Szymański, Paweł, Markowicz, Magdalena, Mikiciuk-Olasik, Elżbieta·2011·International Journal of Molecular Sciences
DOI 10.3390/ijms13010427
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Abstract

High-throughput screening (HTS) is one of the newest techniques used in drug design and may be applied in biological and chemical sciences. This method, due to utilization of robots, detectors and software that regulate the whole process, enables a series of analyses of chemical compounds to be conducted in a short time and the affinity of biological structures which is often related to toxicity to be defined. Since 2008 we have implemented the automation of this technique and as a consequence, the possibility to examine 100,000 compounds per day. The HTS method is more frequently utilized in conjunction with analytical techniques such as NMR or coupled methods e.g., LC-MS/MS. Series of studies enable the establishment of the rate of affinity for targets or the level of toxicity. Moreover, researches are conducted concerning conjugation of nanoparticles with drugs and the determination of the toxicity of such structures. For these purposes there are frequently used cell lines. Due to the miniaturization of all systems, it is possible to examine the compound’s toxicity having only 1–3 mg of this compound. Determination of cytotoxicity in this way leads to a significant decrease in the expenditure and to a reduction in the length of the study.

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Metadata

Authors
Szymański, P., Markowicz, M., & Mikiciuk-Olasik, E.
Venue
International Journal of Molecular Sciences
Publisher
MDPI AG
Published
2011-12-29
Volume
13
Issue
1
Pages
427-452
ISSN
1422-0067 (ISSN-L)
DOI
10.3390/ijms13010427
OpenAlex
W1971375063
Cited by
454
References
90
FWCI
4.90
Type
journal article
Language
en
Open access
gold
Licence
creativecommons.org/licenses/by/3.0/ (vor)

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CoChat. (2026). Citation check: Adaptation of High-Throughput Screening in Drug Discovery—Toxicological Screening Tests. https://app.cochat.ai/papers/10.3390/ijms13010427
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Szymański, P., Markowicz, M., & Mikiciuk-Olasik, E. (2011). Adaptation of High-Throughput Screening in Drug Discovery—Toxicological Screening Tests. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms13010427

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