The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Spliceosome assembly in yeast.
Open accessAbstract
Precursors to mRNA become substrates for splicing by being assembled into a complex multisubunit structure, the spliceosome. To study the assembly of the yeast spliceosome, intermediate complexes were separated by electrophoresis on nondenaturing polyacrylamide gels. Four splicing-dependent complexes, A1, A2-1, A2-2, and B, were observed. The order of assembly of these complexes was determined to be B----A2-1----A1----A2-2. The assembly process can be blocked at complex A1 by addition of 5 mM EDTA or by carrying out the assembly process in heat-inactivated rna2 extracts. The snRNA composition of the complexes was determined by hybridization with probes for five yeast snRNAs. snR14 (U4) was only found in complex A2-1, snR6 (U6) and snR7 (U5) were in complexes A1, A2-1, and A2-2, whereas snR20 (U2) was in all four of the complexes. snR19 (U1) was not present in any of the complexes. Hybridization with these probes was also employed to detect snRNPs present in yeast splicing extracts. We found that snR6, snR7, and snR14 were present together in a large complex. This complex underwent an ATP-dependent dissociation to give snR7 and snR6-snR14 complexes. snR19 and snR20 are present in distinct RNPs but the mobility of these is not affected by ATP. A mechanism for spliceosome assembly is proposed.
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Cites
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A compensatory base change in U1 snRNA suppresses a 5′ splice site mutation
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Structure of spliceosomal snRNPs and their role in pre-mRNA splicing
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Transcription-dependent colocalization of the U1, U2, U4/U6, and U5 snRNPs in coiled bodies
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Metadata
- Authors
- Cheng, S. C., & Abelson, J.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1987-11
- Volume
- 1
- Issue
- 9
- Pages
- 1014-1027
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W1980424685
- Cited by
- 366
- References
- 33
- Type
- posted content
- Language
- en
- Open access
- diamond
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