The DOI matches its Crossref record; 54 of the 54 references it cites were found in a registry or an index.
Normal human chromosomes have long G-rich telomeric overhangs at one end
Open accessAbstract
Telomeres protect the ends of linear chromosomes from degradation and abnormal recombination events, and in vertebrates may be important in cellular senescence and cancer. However, very little is known about the structure of human telomeres. In this report we purify telomeres and analyze their termini. We show that following replication the daughter telomeres have different terminal overhangs in normal diploid telomerase-negative human fibroblasts. Electron microscopy of those telomeres that have long overhangs yields 200 ± 75 nucleotides of single-stranded DNA. This overhang is four times greater than the amount of telomere shortening per division found in these cells. These results are consistent with models of telomere replication in which leading-strand synthesis generates a blunt end while lagging-strand synthesis produces a long G-rich 3′ overhang, and suggest that variations in lagging-strand synthesis may regulate the rate of telomere shortening in normal diploid human cells. Our results do not exclude the possibility that nuclease processing events following leading strand synthesis result in short overhangs on one end.
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Cites
Telomeres shorten during ageing of human fibroblasts
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A survey of telomerase activity in human cancer
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Self-citationThe RNA Component of Human Telomerase
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A highly conserved repetitive DNA sequence,(TTAGGG) n, present at the telomeres of human chromosomes
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Telomere reduction in human colorectal carcinoma and with ageing
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A telomeric sequence in the RNA of Tetrahymena telomerase required for telomere repeat synthesis
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“Telomerase contains reverse transcriptase motifs (Lingner et al. 1997), and using its RNA component as a template (Greider and Blackburn 1989), it can add repetitive sequences to the 38 end of the chromosomes.”
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Reverse Transcriptase Motifs in the Catalytic Subunit of Telomerase
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Cited by
TRF2 Protects Human Telomeres from End-to-End Fusions
van Steensel, B., Smogorzewska, A., & de Lange, T. (1998) · Cell · doi:10.1016/s0092-8674(00)80932-0 · Cited by 1,709
Pot1, the Putative Telomere End-Binding Protein in Fission Yeast and Humans
Baumann, P., & Cech, T. R. (2001) · Science · doi:10.1126/science.1060036 · Cited by 1,028
Protection of mammalian telomeres
de Lange, T. (2002) · Oncogene · doi:10.1038/sj.onc.1205080 · Cited by 942
Human Telomerase and Its Regulation
Cong, Y.-S., Wright, W. E., & Shay, J. W. (2002) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.66.3.407-425.2002 · Cited by 898
Self-citationTelomere maintenance as a target for anticancer drug discovery
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Metadata
- Authors
- Wright, W. E., Tesmer, V. M., Huffman, K. E., Levene, S. D., & Shay, J. W.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1997-11-01
- Volume
- 11
- Issue
- 21
- Pages
- 2801-2809
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2133038385
- Cited by
- 734
- References
- 54
- Type
- posted content
- Language
- en
- Open access
- diamond
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- Wright, W. E., Tesmer, V. M., Huffman, K. E., Levene, S. D., & Shay, J. W. (1997). Normal human chromosomes have long G-rich telomeric overhangs at one end. Genes & Development. https://doi.org/10.1101/gad.11.21.2801
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
