The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
An mRNA structure in bacteria that controls gene expression by binding lysine
Open accessAbstract
Riboswitches are metabolite-responsive genetic control elements that reside in the untranslated regions (UTRs) of certain messenger RNAs. Herein, we report that the 5′-UTR of the lysC gene of Bacillus subtilis carries a conserved RNA element that serves as a lysine-responsive riboswitch. The ligand-binding domain of the riboswitch binds to L-lysine with an apparent dissociation constant (K D) of ∼1 µM, and exhibits a high level of molecular discrimination against closely related analogs, including D-lysine and ornithine. Furthermore, we provide evidence that this widespread class of riboswitches serves as a target for the antimetabolite S-(2-aminoethyl)-L-cysteine (AEC). These findings add support to the hypotheses that direct sensing of metabolites by messenger RNAs is a fundamental form of genetic control and that riboswitches represent a new class of antimicrobial drug targets.
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Cites
Thiamine derivatives bind messenger RNAs directly to regulate bacterial gene expression
Winkler, W., Nahvi, A., & Breaker, R. R. (2002) · Nature · doi:10.1038/nature01145 · Cited by 1,242
Self-citationGenetic Control by a Metabolite Binding mRNA
Nahvi, A., Sudarsan, N., Ebert, M. S., Zou, X., Brown, K. L., & Breaker, R. R. (2002) · Chemistry & Biology · doi:10.1016/s1074-5521(02)00224-7 · Cited by 801
Self-citationRiboswitches Control Fundamental Biochemical Pathways in Bacillus subtilis and Other Bacteria
Mandal, M., Boese, B., Barrick, J. E., Winkler, W. C., & Breaker, R. R. (2003) · Cell · doi:10.1016/s0092-8674(03)00391-x · Cited by 726
Self-citationAn mRNA structure that controls gene expression by binding FMN
Winkler, W. C., Cohen-Chalamish, S., & Breaker, R. R. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.212628899 · Cited by 696
Self-citationSensing Small Molecules by Nascent RNA
Mironov, A. S., Gusarov, I., Rafikov, R., Lopez, L. E., Shatalin, K., Kreneva, R. A., Perumov, D. A., & Nudler, E. (2002) · Cell · doi:10.1016/s0092-8674(02)01134-0 · Cited by 682
Bacillus Subtilis and Its Closest Relatives: From Genes to Cells
Sonenshein, A. L., Hoch, J. A., & Losick, R. M. (2001) · Cited by 674
Relationship between internucleotide linkage geometry and the stability of RNA
SOUKUP, G. A., & BREAKER, R. R. (1999) · RNA · doi:10.1017/s1355838299990891 · Cited by 549
Self-citationAn mRNA structure that controls gene expression by binding S-adenosylmethionine
Winkler, W. C., Nahvi, A., Sudarsan, N., Barrick, J. E., & Breaker, R. R. (2003) · Nature Structural & Molecular Biology · doi:10.1038/nsb967 · Cited by 459
Self-citationMetabolite-binding RNA domains are present in the genes of eukaryotes
SUDARSAN, N., BARRICK, J. E., & BREAKER, R. R. (2003) · RNA · doi:10.1261/rna.5090103 · Cited by 420
Self-citationPtashne, M., & Gann, A. A. F. (2001) · Cold Spring Harbor Laboratory Institutional Repository (Cold Spring Harbor Laboratory) · Cited by 356
Cited by
Adenine riboswitches and gene activation by disruption of a transcription terminator
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Self-citationProspects for riboswitch discovery and analysis
Breaker, R. R. (2011) · Molecular Cell · doi:10.1016/j.molcel.2011.08.024 · Cited by 464
Self-citationStructural basis for gene regulation by a thiamine pyrophosphate-sensing riboswitch
Serganov, A., Polonskaia, A., Phan, A. T., Breaker, R. R., & Patel, D. J. (2006) · Nature · doi:10.1038/nature04740 · Cited by 441
Self-citationWidespread genetic switches and toxicity resistance proteins for fluoride
Baker, J. L., Sudarsan, N., Weinberg, Z., Roth, A., Stockbridge, R. B., & Breaker, R. R. (2012) · Science · doi:10.1126/science.1215063 · Cited by 438
Self-citationAn RNA Sensor for Intracellular Mg2+
Cromie, M. J., Shi, Y., Latifi, T., & Groisman, E. A. (2006) · Cell · doi:10.1016/j.cell.2006.01.043 · Cited by 323
Metadata
- Authors
- Sudarsan, N., Wickiser, J. K., Nakamura, S., Ebert, M. S., & Breaker, R. R.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2003-11-01
- Volume
- 17
- Issue
- 21
- Pages
- 2688-2697
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2131948524
- Cited by
- 357
- References
- 30
- Type
- posted content
- Language
- en
- Open access
- diamond
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- Sudarsan, N., Wickiser, J. K., Nakamura, S., Ebert, M. S., & Breaker, R. R. (2003). An mRNA structure in bacteria that controls gene expression by binding lysine. Genes & Development. https://doi.org/10.1101/gad.1140003
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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