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The essential Gcd10p–Gcd14p nuclear complex is required for 1-methyladenosine modification and maturation of initiator methionyl-tRNA
Open accessAbstract
Gcd10p and Gcd14p are essential proteins required for the initiation of protein synthesis and translational repression of GCN4 mRNA. The phenotypes of gcd10 mutants were suppressed by high-copy-number IMT genes, encoding initiator methionyl tRNA (tRNA iMet), or LHP1, encoding the yeast homolog of the human La autoantigen. The gcd10-504 mutation led to a reduction in steady-state levels of mature tRNA iMet, attributable to increased turnover rather than decreased synthesis of pre-tRNA iMet. Remarkably, the lethality of a GCD10 deletion was suppressed by high-copy-number IMT4, indicating that its role in expression of mature tRNA iMet is the essential function of Gcd10p. A gcd14-2 mutant also showed reduced amounts of mature tRNA iMet, but in addition, displayed a defect in pre-tRNA iMet processing. Gcd10p and Gcd14p were found to be subunits of a protein complex with prominent nuclear localization, suggesting a direct role in tRNA iMet maturation. The chromatographic behavior of elongator and initiator tRNA Met on a RPC-5 column indicated that both species are altered structurally in gcd10Δ cells, and analysis of base modifications revealed that 1-methyladenosine (m 1 A) is undetectable in gcd10Δ tRNA. Interestingly,gcd10 and gcd14 mutations had no effect on processing or accumulation of elongator tRNA Met, which also contains m 1 A at position 58, suggesting a unique requirement for this base modification in initiator maturation.
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Cited by
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Comparative genomics and evolution of proteins involved in RNA metabolism
Anantharaman, V. (2002) · Nucleic Acids Research · doi:10.1093/nar/30.7.1427 · Cited by 541
Cozen, A. E., Quartley, E., Holmes, A. D., Hrabeta-Robinson, E., Phizicky, E. M., & Lowe, T. M. (2015) · Nature Methods · doi:10.1038/nmeth.3508 · Cited by 479
RNA Methylation in ncRNA: Classes, Detection, and Molecular Associations
Romano, G., Veneziano, D., Nigita, G., & Nana-Sinkam, S. P. (2018) · Frontiers in Genetics · doi:10.3389/fgene.2018.00243 · Cited by 49
Metadata
- Authors
- Anderson, J., Phan, L., Cuesta, R., Carlson, B. A., Pak, M., Asano, K., Björk, G. R., Tamame, M., & Hinnebusch, A. G.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1998-12-01
- Volume
- 12
- Issue
- 23
- Pages
- 3650-3662
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2113263393
- Cited by
- 281
- References
- 0
- Type
- posted content
- Language
- en
- Open access
- diamond
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- CoChat. (2026). Citation check: The essential Gcd10p–Gcd14p nuclear complex is required for 1-methyladenosine modification and maturation of initiator methionyl-tRNA. https://app.cochat.ai/papers/10.1101/gad.12.23.3650
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- Anderson, J., Phan, L., Cuesta, R., Carlson, B. A., Pak, M., Asano, K., Björk, G. R., Tamame, M., & Hinnebusch, A. G. (1998). The essential Gcd10p–Gcd14p nuclear complex is required for 1-methyladenosine modification and maturation of initiator methionyl-tRNA. Genes & Development. https://doi.org/10.1101/gad.12.23.3650
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