The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
A Rik1-associated, cullin-dependent E3 ubiquitin ligase is essential for heterochromatin formation
Open accessAbstract
Heterochromatin is critical for proper centromere and telomere function, and it plays a key role in the transcriptional silencing of specific genomic loci. In fission yeast, the Rik1 protein functions with the Clr4 histone methyltransferase at an early step in heterochromatin formation. Here, we use mass spectrometry and tandem affinity purification of a Rik1-TAP fusion protein to identify Rik1-associated proteins. These studies identify two novel proteins, Raf1 and Raf2, which we find are required for H3-K9 methylation and for transcriptional silencing within centromeric heterochromatin. We also find that subunits of a cullin-dependent E3 ubiquitin ligase are associated with Rik1 and Clr4, and Rik1-TAP preparations exhibit robust E3 ubiquitin ligase activity. Furthermore, expression of a dominant-negative allele of the Pcu4 cullin subunit disrupts regulation of K4 methylation within heterochromatin. These studies provide evidence for a novel Rik1-associated E3 ubiquitin ligase that is required for heterochromatin formation.
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Cites
Function and regulation of cullin–RING ubiquitin ligases
Petroski, M. D., & Deshaies, R. J. (2005) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1547 · Cited by 2,148
Structure of the Cul1–Rbx1–Skp1–F boxSkp2 SCF ubiquitin ligase complex
Zheng, N., Schulman, B. A., Song, L., Miller, J. J., Jeffrey, P. D., Wang, P., Chu, C., Koepp, D. M., Elledge, S. J., Pagano, M., et al. (2002) · Nature · doi:10.1038/416703a · Cited by 1,507
Heterochromatin and Epigenetic Control of Gene Expression
Grewal, S. I. S., & Moazed, D. (2003) · Science · doi:10.1126/science.1086887 · Cited by 1,065
Chromosomal Inheritance of Epigenetic States in Fission Yeast During Mitosis and Meiosis
Grewal, S. I. S., & Klar, A. J. S. (1996) · Cell · doi:10.1016/s0092-8674(00)80080-x · Cited by 260
Cited by
Grewal, S. I. S., & Jia, S. (2007) · Nature Reviews Genetics · doi:10.1038/nrg2008 · Cited by 1,263
Spreading of silent chromatin: inaction at a distance
Talbert, P. B., & Henikoff, S. (2006) · Nature Reviews Genetics · doi:10.1038/nrg1920 · Cited by 300
Bosch-Presegué, L., Raurell-Vila, H., Marazuela-Duque, A., Kane-Goldsmith, N., Valle, A., Oliver, J., Serrano, L., & Vaquero, A. (2011) · Molecular Cell · doi:10.1016/j.molcel.2011.02.034 · Cited by 138
Common themes in siRNA-mediated epigenetic silencing pathways
Verdel, A., Vavasseur, A., Le Gorrec, M., & Touat-Todeschini, L. (2009) · The International Journal of Developmental Biology · doi:10.1387/ijdb.082691av · Cited by 135
The Methyltransferase Activity of Clr4Suv39h Triggers RNAi Independently of Histone H3K9 Methylation
Gerace, E. L., Halic, M., & Moazed, D. (2010) · Molecular Cell · doi:10.1016/j.molcel.2010.07.017 · Cited by 70
Metadata
- Authors
- Horn, P. J., Bastie, J.-N., & Peterson, C. L.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2005-07-15
- Volume
- 19
- Issue
- 14
- Pages
- 1705-1714
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2147500564
- Cited by
- 188
- References
- 4
- Type
- posted content
- Language
- en
- Open access
- diamond
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