The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
The checkpoint protein Ddc2, functionally related to S. pombe Rad26, interacts with Mec1 and is regulated by Mec1-dependent phosphorylation in budding yeast
Open accessAbstract
DDC2 is a novel component of the DNA integrity checkpoint pathway, which is required for proper checkpoint response to DNA damage and to incomplete DNA replication. Moreover, Ddc2 overproduction causes sensitivity to DNA-damaging agents and checkpoint defects. Ddc2 physically interacts with Mec1 and undergoes Mec1-dependent phosphorylation both in vitro and in vivo. The phosphorylation of Ddc2 takes place in late S phase and in G 2 phase during an unperturbed cell cycle and is further increased in response to DNA damage. Because Ddc2 phosphorylation does not require any other known tested checkpoint factors but Mec1, the Ddc2–Mec1 complex might respond to the presence of some DNA structures independently of the other known checkpoint proteins. Our findings suggest that Ddc2 may be the functional homolog of Schizosaccharomyces pombe Rad26, strengthening the hypothesis that the mechanisms leading to checkpoint activation are conserved throughout evolution.
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Cites
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“Ddc1, Rad9, and Rad53 are phosphorylated immediately in response to DNA damage at any phase of the cell cycle (Sanchez et al. 1996; Sun et al. 1996; Longhese et al. 1997; Emili 1998; Paciotti et al. 1998; Sun et al. 1998; Vialard et al. 1998), while DNA damage-induced phosphorylation of Ddc2 occurs…”
BackgroundInfluentialThe controlling role of ATM in homologous recombinational repair of DNA damage
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Cited by
The DNA damage response: putting checkpoints in perspective
Zhou, B.-B. S., & Elledge, S. J. (2000) · Nature · doi:10.1038/35044005 · Cited by 3,213
Sensing DNA Damage Through ATRIP Recognition of RPA-ssDNA Complexes
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ATM and related protein kinases: safeguarding genome integrity
Shiloh, Y. (2003) · Nature Reviews Cancer · doi:10.1038/nrc1011 · Cited by 2,577
Cell cycle checkpoint signaling through the ATM and ATR kinases
Abraham, R. T. (2001) · Genes & Development · doi:10.1101/gad.914401 · Cited by 1,966
ATR and ATRIP: Partners in Checkpoint Signaling
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Metadata
- Authors
- Paciotti, V., Clerici, M., Lucchini, G., & Longhese, M. P.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2000-08-15
- Volume
- 14
- Issue
- 16
- Pages
- 2046-2059
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W1961560739
- Cited by
- 246
- References
- 9
- Type
- posted content
- Language
- en
- Open access
- diamond
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- CoChat. (2026). Citation check: The checkpoint protein Ddc2, functionally related to S. pombe Rad26, interacts with Mec1 and is regulated by Mec1-dependent phosphorylation in budding yeast. https://app.cochat.ai/papers/10.1101/gad.14.16.2046
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- Paciotti, V., Clerici, M., Lucchini, G., & Longhese, M. P. (2000). The checkpoint protein Ddc2, functionally related to S. pombe Rad26, interacts with Mec1 and is regulated by Mec1-dependent phosphorylation in budding yeast. Genes & Development. https://doi.org/10.1101/gad.14.16.2046
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