Cited by 164DNA Repair MechanismsFungal and yeast genetics researchBacterial Genetics and Biotechnology

DNA repair protein Rad55 is a terminal substrate of the DNA damage checkpoints

Open access
Bashkirov, Vladimir I., King, Jeff S., Bashkirova, Elena V., Schmuckli-Maurer, Jacqueline, Heyer, Wolf-Dietrich·2000·Molecular and Cellular Biology

Abstract

Checkpoints, which are integral to the cellular response to DNA damage, coordinate transient cell cycle arrest and the induced expression of DNA repair genes after genotoxic stress. DNA repair ensures cellular survival and genomic stability, utilizing a multipathway network. Here we report evidence that the two systems, DNA damage checkpoint control and DNA repair, are directly connected by demonstrating that the Rad55 double-strand break repair protein of the recombinational repair pathway is a terminal substrate of DNA damage and replication block checkpoints. Rad55p was specifically phosphorylated in response to DNA damage induced by the alkylating agent methyl methanesulfonate, dependent on an active DNA damage checkpoint. Rad55p modification was also observed after gamma ray and UV radiation. The rapid time course of phosphorylation and the recombination defects identified in checkpoint-deficient cells are consistent with a role of the DNA damage checkpoint in activating recombinational repair. Rad55p phosphorylation possibly affects the balance between different competing DNA repair pathways.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1128/mcb.20.12.4393-4404.2000

Cites

98 of 100 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Checkpoints actively stimulate repair processes and, in higher eukaryotes, trigger apoptosis when damage cannot be successfully dealt with.

    “The checkpoints also actively stimulate the repair processes [1–9] and, in higher eukaryotes, trigger the apoptotic response, if damage cannot be dealt with successfully [10–12].”

    From Mind the gap: keeping UV lesions in check (2011) · cites this paper as [3]

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Bashkirov, V. I., King, J. S., Bashkirova, E. V., Schmuckli-Maurer, J., & Heyer, W.-D.
Affiliations
  • Russian Academy of Sciences (RU)
  • Institute of General Microbiology
  • University of California, Davis (US)
Corresponding author
Heyer, Wolf-Dietrich
Venue
Molecular and Cellular Biology
Published
2000-06-01
Volume
20
Issue
12
Pages
4393-4404
ISSN
  • 0270-7306 (ISSN-L)
  • 1098-5549
OpenAlex
W2121218667
Cited by
164
References
98
Citations by year
2026: 2 · 2025: 2 · 2024: 3 · 2023: 3 · 2022: 1 · 2021: 4 · 2019: 1 · 2018: 3 · 2017: 1 · 2016: 9 · 2015: 1 · 2014: 3 · 2013: 1 · 2012: 7
FWCI
7.11
Type
journal article
Language
en
Open access
Locations
  • Molecular and Cellular Biology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
Rad55, DNA damage checkpoint, DNA repair, phosphorylation, recombinational repair, DNA double-strand break repair, methyl methanesulfonate, gamma radiation, ultraviolet radiation, recombination defects, cell cycle arrest, genome stability
MeSH terms
DNA Damage, DNA Repair, DNA-Binding Proteins, DNA, Fungal, Fungal Proteins, Phosphorylation, Recombination, Genetic, Saccharomyces cerevisiae, Ultraviolet Rays, Gene Expression Regulation, Fungal, Saccharomyces cerevisiae Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review