The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Compaction of chromatin by diverse Polycomb group proteins requires localized regions of high charge
Open accessAbstract
Polycomb group (PcG) proteins are required for the epigenetic maintenance of developmental genes in a silent state. Proteins in the Polycomb-repressive complex 1 (PRC1) class of the PcG are conserved from flies to humans and inhibit transcription. One hypothesis for PRC1 mechanism is that it compacts chromatin, based in part on electron microscopy experiments demonstrating that Drosophila PRC1 compacts nucleosomal arrays. We show that this function is conserved between Drosophila and mouse PRC1 complexes and requires a region with an overrepresentation of basic amino acids. While the active region is found in the Posterior Sex Combs (PSC) subunit in Drosophila, it is unexpectedly found in a different PRC1 subunit, a Polycomb homolog called M33, in mice. We provide experimental support for the general importance of a charged region by predicting the compacting capability of PcG proteins from species other than Drosophila and mice and by testing several of these proteins using solution assays and microscopy. We infer that the ability of PcG proteins to compact chromatin in vitro can be predicted by the presence of domains of high positive charge and that PRC1 components from a variety of species conserve this highly charged region. This supports the hypothesis that compaction is a key aspect of PcG function.
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Biopython: freely available python tools for computational molecular biology and bioinformatics
Cock, P. J. A., Antao, T., Chang, J. T., Chapman, B. A., Cox, C. J., Dalke, A., Friedberg, I., Hamelryck, T., Kauff, F., Wilczynski, B., et al. (2009) · Bioinformatics · doi:10.1093/bioinformatics/btp163 · Cited by 6,080
Self-citationKuzmichev, A., Nishioka, K., Erdjument-Bromage, H., Tempst, P., & Reinberg, D. (2002) · Genes & Development · doi:10.1101/gad.1035902 · Cited by 1,596
Cited by
Genome Regulation by Polycomb and Trithorax: 70 Years and Counting
Schuettengruber, B., Bourbon, H.-M., Di Croce, L., & Cavalli, G. (2017) · Cell · doi:10.1016/j.cell.2017.08.002 · Cited by 1,094
Simon, J. A., & Kingston, R. E. (2013) · Molecular Cell · doi:10.1016/j.molcel.2013.02.013 · Cited by 733
Self-citationNucleosome structure and function
McGinty, R. K., & Tan, S. (2015) · Chemical Reviews · doi:10.1021/cr500373h · Cited by 530
Kundu, S., Ji, F., Sunwoo, H., Jain, G., Lee, J. T., Sadreyev, R. I., Dekker, J., & Kingston, R. E. (2017) · Molecular Cell · doi:10.1016/j.molcel.2017.01.009 · Cited by 333
“…(H2AK119Ub), create compacted chromatin structures in vitro, and bring together distant genomic regions in vivo (Bantignies et al., 2011; Buchwald et al., 2006; Cheutin andCavalli, 2014; Eskeland et al., 2010; Francis et al., 2004; Grau et al., 2011; Schoenfelder et al., 2015; Wang et al., 2004).”
BackgroundInfluentialSelf-citationSAM Domain Polymerization Links Subnuclear Clustering of PRC1 to Gene Silencing
Isono, K., Endo, T. A., Ku, M., Yamada, D., Suzuki, R., Sharif, J., Ishikura, T., Toyoda, T., Bernstein, B. E., & Koseki, H. (2013) · Developmental Cell · doi:10.1016/j.devcel.2013.08.016 · Cited by 309
Metadata
- Authors
- Grau, D. J., Chapman, B. A., Garlick, J. D., Borowsky, M., Francis, N. J., & Kingston, R. E.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2011-10-15
- Volume
- 25
- Issue
- 20
- Pages
- 2210-2221
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2098500735
- Cited by
- 251
- References
- 2
- Type
- posted content
- Language
- en
- Open access
- diamond
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- Grau, D. J., Chapman, B. A., Garlick, J. D., Borowsky, M., Francis, N. J., & Kingston, R. E. (2011). Compaction of chromatin by diverse Polycomb group proteins requires localized regions of high charge. Genes & Development. https://doi.org/10.1101/gad.17288211
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