The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
Mammalian pre-mRNA branch site selection by U2 snRNP involves base pairing.
Open accessAbstract
SV40 early pre-mRNA is alternatively spliced to produce large T and small t mRNAs by use of different 5'-splice sites and a shared 3'-splice site. The large T splicing pathway uses multiple lariat branch sites, whereas small t splicing, constrained by its small intron size, can use only one. We exploited this situation to test the hypothesis that RNA-RNA base pairing between U2 snRNA and the branch site sequence is important in mammalian pre-mRNA splicing by constructing and analyzing several mutations in the small t pre-mRNA branch site (UUCUAAU). All of the mutations resulted in substantial decreases in small t splicing relative to large T. To test whether these effects resulted from decreased base pairing with U2 snRNA, compensatory mutations were introduced at the appropriate positions (nucleotides 34-36) in a cloned human U2 gene. All branch site mutations tested (four separate single base substitutions and two triple mutations) were suppressed (i.e., small t splicing was increased) by the appropriate U2 mutations. These results establish that recognition of the poorly conserved mammalian pre-mRNA branch site sequence by U2 snRNP can involve base-pairing.
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Metadata
- Authors
- Wu, J., & Manley, J. L.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1989-10
- Volume
- 3
- Issue
- 10
- Pages
- 1553-1561
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2133143001
- Cited by
- 293
- References
- 37
- Type
- posted content
- Language
- en
- Open access
- diamond
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