The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Definition of an efficient synthetic poly(A) site.
Open accessAbstract
We constructed and analyzed a synthetic poly(A) (SPA) site that was based on the highly efficient poly(A) signal of the rabbit beta-globin gene. By use of the SPA, we demonstrate that the minimum sequences required for efficient polyadenylation are the AATAAA sequence and a GT/T-rich sequence with the correct spacing of 22-23 nucleotides between them. When placed downstream of the poly(A) site of the human alpha 2-globin gene, the SPA is used exclusively. We predict that the SPA, with its more extensive GT/T-rich sequence, is a more efficient poly(A) site than alpha-globin. Also, we compared the use of the SPA when it is placed either in the exon 3 or intron 2 of the rabbit beta-globin gene. When in the exonic position, SPA is used 10-fold more than the regular poly(A) site of rabbit beta-globin. In contrast, when it is in the intronic location, no detectable use of SPA is observed; however, the deletion of the donor site of intron 2 reactivates the intronic positioned SPA. These results indicate that the splicing of intron 2 in the rabbit beta-globin gene occurs ahead of polyadenylation and have important implications for termination of transcription. Polyadenylation, although required for termination of transcription, is not sufficient; therefore, additional termination signals for RNA polymerase II must exist.
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The sequence 5′-AAUAAA-3′ forms part of the recognition site for polyadenylation of late SV40 mRNAs
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DNA sequences necessary for transcription of the rabbit β-globin gene in vivo
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Metadata
- Authors
- Levitt, N., Briggs, D., Gil, A., & Proudfoot, N. J.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1989-07
- Volume
- 3
- Issue
- 7
- Pages
- 1019-1025
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2004635090
- Cited by
- 290
- References
- 22
- Type
- posted content
- Language
- en
- Open access
- diamond
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