Rec8-containing cohesin maintains bivalents without turnover during the growing phase of mouse oocytes
Open accessAbstract
During female meiosis, bivalent chromosomes are thought to be held together from birth until ovulation by sister chromatid cohesion mediated by cohesin complexes whose ring structure depends on kleisin subunits, either Rec8 or Scc1. Because cohesion is established at DNA replication in the embryo, its maintenance for such a long time may require cohesin turnover. To address whether Rec8- or Scc1-containing cohesin holds bivalents together and whether it turns over, we created mice whose kleisin subunits can be cleaved by TEV protease. We show by microinjection experiments and confocal live-cell imaging that Rec8 cleavage triggers chiasmata resolution during meiosis I and sister centromere disjunction during meiosis II, while Scc1 cleavage triggers sister chromatid disjunction in the first embryonic mitosis, demonstrating a dramatic transition from Rec8- to Scc1-containing cohesin at fertilization. Crucially, activation of an ectopic Rec8 transgene during the growing phase of Rec8 TEV / TEV oocytes does not prevent TEV-mediated bivalent destruction, implying little or no cohesin turnover for ≥2 wk during oocyte growth. We suggest that the inability of oocytes to regenerate cohesion may contribute to age-related meiosis I errors.
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For proteins with very slow turnover rates that surpass 48-72 h, RNAi approaches will not yield the desired knockdown of target transcripts.
“However, for those proteins that have very slow turnover rates that surpass this time frame [7], RNAi approaches will not yield the desired knockdown of target transcripts.”
From Advances in targeted degradation of endogenous proteins (2019) · cites this paper as [7]
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- Authors
- Tachibana-Konwalski, K., Godwin, J., van der Weyden, L., Champion, L., Kudo, N. R., Adams, D. J., & Nasmyth, K.
- Affiliations
- University of Oxford (GB)
- Imperial College London (GB)
- Wellcome Sanger Institute (GB)
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2010-10-22
- Volume
- 24
- Issue
- 22
- Pages
- 2505-2516
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- PMC
- PMC2975927
- OpenAlex
- W2120439612
- Cited by
- 265
- References
- 61
- Citations by year
- 2026: 18 · 2025: 13 · 2024: 12 · 2023: 9 · 2022: 14 · 2021: 20 · 2020: 13 · 2019: 14 · 2018: 16 · 2017: 26 · 2016: 19 · 2015: 15 · 2014: 13 · 2013: 21 · 2012: 24
- Type
- posted content
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- other-oa
- Locations
- Genes & Development (publishedVersion, other-oa)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Wellcome Trust
- Cancer Research UK
- Kay Kendall Leukaemia Fund
- Medical Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Keywords
- REC8, Scc1, cohesin, sister chromatid cohesion, bivalent chromosomes, oocyte meiosis, oocyte growth, TEV protease cleavage, meiotic errors, mouse oocytes
- MeSH terms
- Cohesins, Animals, Cells, Cultured, Centromere, Chromosomal Proteins, Non-Histone, Chromosomes, Female, Nuclear Proteins, Oocytes, Endopeptidases, Phosphoproteins, Cell Cycle Proteins, Mice
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