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Cited by 275

The DOI matches its Crossref record; 59 of the 59 references it cites were found in a registry or an index.

Cloning of yeast HAP5: a novel subunit of a heterotrimeric complex required for CCAAT binding.

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McNabb, D S, Xing, Y, Guarente, L·1995·Genes & Development
DOI 10.1101/gad.9.1.47
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Abstract

The CCAAT-binding factor is a conserved heteromeric transcription factor that binds to CCAAT box-containing upstream activation sites (UASs) within the promoters of numerous eukaryotic genes. The CCAAT-binding factor of Saccharomyces cerevisiae activates the transcription of these genes in response to growth in a nonfermentable carbon source. Previous studies have demonstrated that the HAP2, HAP3, and HAP4 subunits of the yeast CCAAT-binding factor are required for the transcriptional activation of genes containing a CCAAT box. Using the two-hybrid screening method, we have identified an additional component of the CCAAT-binding factor. We present the identification and characterization of a novel gene, HAP5, that encodes an additional subunit of the CCAAT-binding factor required for the assembly and DNA-binding activity of the complex. In a hap5 mutant, we show that CCAAT-binding activity is abolished in vitro. Furthermore, we demonstrate that purified recombinant HAP2, HAP3, and HAP5 are able to reconstitute CCAAT-binding activity in mobility shift analysis. These data suggest that the HAP2/3/5 heterotrimer represents a unique DNA-binding factor in which all three subunits of the complex are absolutely required for DNA-binding activity.

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Metadata

Authors
McNabb, D. S., Xing, Y., & Guarente, L.
Venue
Genes & Development
Publisher
Cold Spring Harbor Laboratory
Published
1995-01-01
Volume
9
Issue
1
Pages
47-58
ISSN
  • 0890-9369 (ISSN-L)
  • 1549-5477
DOI
10.1101/gad.9.1.47
OpenAlex
W2037802059
Cited by
275
References
59
Type
posted content
Language
en
Open access
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CoChat. (2026). Citation check: Cloning of yeast HAP5: a novel subunit of a heterotrimeric complex required for CCAAT binding. https://app.cochat.ai/papers/10.1101/gad.9.1.47
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McNabb, D. S., Xing, Y., & Guarente, L. (1995). Cloning of yeast HAP5: a novel subunit of a heterotrimeric complex required for CCAAT binding. Genes & Development. https://doi.org/10.1101/gad.9.1.47

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.