Enhancer–promoter specificity mediated by DPE or TATA core promoter motifs
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To investigate the basis for enhancer–promoter specificity, we compared the ability of enhancers to activate transcription in vivo from core promoters containing either downstream promoter element (DPE) or TATA box motifs. To eliminate position effects, we generated and analyzed pairs of sister Drosophila lines that contain a DPE- or TATA-dependent reporter gene at precisely the same genomic position relative to each enhancer. These studies revealed transcriptional enhancers that are specific for promoters that contain either DPE or TATA box elements. Thus, the core promoter not only mediates the initiation of transcription, but also functions as a regulatory element.
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Enhancer trap experiments in Drosophila showed that some endogenous enhancers had a preference for either a TATA- or a DPE-containing promoter.
“enhancer trap experiments in Drosophila that used randomly integrated reporter genes driven by a TATA- and a DPE-containing promoter, respectively, showed that some endogenous enhancers had a preference for either of the two promoters [475].”
From Transcriptional enhancers and their communication with gene promoters (2021) · cites this paper as [475]
Some enhancers show a preference for certain types of promoters.
“Some enhancers show a preference for certain types of promoters. Enhancers were classified according to their ability to preferentially activate promoters containing a DPE (downstream promoter element) or promoters containing a TATA box [133].”
From Enhancer Function in the 3D Genome (2023) · cites this paper as [133]
Enhancers were classified according to their ability to preferentially activate promoters containing a DPE or promoters containing a TATA box.
“Enhancers were classified according to their ability to preferentially activate promoters containing a DPE (downstream promoter element) or promoters containing a TATA box [133].”
From Enhancer Function in the 3D Genome (2023) · cites this paper as [133]
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Research works printed but not linked: 5
- Bellen, H.J., O'Kane, C.J., Wilson, C., Grossniklaus, U., Pearson, R.K., and Gehring, W.J. 1989. P-element mediated enhancer detection: A versatile method to study development in Drosophila. Genes & Dev. 3: 1288-1300.
- Garraway, I.P., Semple, K., and Smale, S.T. 1996. Transcription of the lymphocyte-specific terminal deoxynucleotidyltransferase gene re- quires a specific core promoter structure. Proc. Natl. Acad. Sci. 93: 4336-4341.
- O'Kane, C.J. and Gehring, W.J. 1987. Detection in situ of genomic regu- latory elements in Drosophila. Proc. Natl. Acad. Sci. 84: 9123-9127.
- ---. 1997. An experimental test for lineage-specific position effects on alcohol dehydrogenase (Adh) genes in Drosophila. Proc. Natl. Acad. Sci. 95: 15513-15518.
- White, R.J. 2001. Gene transcription: Mechanisms and control. Black- well Science, Ltd., Oxford, UK.
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- Authors
- Butler, J. E. F., & Kadonaga, J. T.
- Affiliations
- University of California San Diego (US)
- Corresponding author
- Kadonaga, James T.
- ORCID
- Kadonaga, James T.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2001-10-01
- Volume
- 15
- Issue
- 19
- Pages
- 2515-2519
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- PMC
- PMC312797
- OpenAlex
- W2101079676
- Cited by
- 265
- References
- 0
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 7 · 2023: 6 · 2022: 12 · 2021: 13 · 2020: 9 · 2019: 7 · 2018: 8 · 2017: 6 · 2016: 16 · 2015: 13 · 2014: 11 · 2013: 11 · 2012: 5
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Genes & Development (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute of General Medical Sciences (R01GM041249, GM41249)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
- Keywords
- downstream promoter element, TATA box, core promoter, enhancers, Drosophila, position effect, transcriptional regulation
- MeSH terms
- Animals, DNA Transposable Elements, Drosophila, Enhancer Elements, Genetic, Promoter Regions, Genetic, TATA Box, Animals, Genetically Modified
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