PreprintCited by 404Bioinformatics and Genomic NetworksAdvanced Proteomics Techniques and ApplicationsBacterial Genetics and Biotechnology

The DOI matches its Crossref record; 41 of the 44 references it cites were found in a registry or an index.

Large-scale identification of protein–protein interaction of Escherichia coli K-12

Open access

Abstract

Protein–protein interactions play key roles in protein function and the structural organization of a cell. A thorough description of these interactions should facilitate elucidation of cellular activities, targeted-drug design, and whole cell engineering. A large-scale comprehensive pull-down assay was performed using a His-tagged Escherichia coli ORF clone library. Of 4339 bait proteins tested, partners were found for 2667, including 779 of unknown function. Proteins copurifying with hexahistidine-tagged baits on a Ni 2+-NTA column were identified by MALDI-TOF MS (matrix-assisted laser desorption ionization time of flight mass spectrometry). An extended analysis of these interacting networks by bioinformatics and experimentation should provide new insights and novel strategies for E. coli systems biology.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1101/gr.4527806

Cites

41 of 41 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Flagged first, then by citations

Show all 44 references

3 of the 44 references couldn’t be matched to a known paper.

Research works printed but not linked: 1

  1. Silver, 2004, Cells by design., Scientist, 18, 30

Cited by

Sorted by citations

Items 201–300 of 404

Metadata

Authors
Arifuzzaman, M., Maeda, M., Itoh, A., Nishikata, K., Takita, C., Saito, R., Ara, T., Nakahigashi, K., Huang, H.-C., Hirai, A., Tsuzuki, K., Nakamura, S., Altaf-Ul-Amin, M., Oshima, T., Baba, T., Yamamoto, N., Kawamura, T., Ioka-Nakamichi, T., Kitagawa, M., Tomita, M., Kanaya, S., Wada, C., & Mori, H.
Affiliations
  • Japan Bioindustry Association (JP)
  • Japan Science and Technology Agency (JP)
  • Institute for Advanced Biosciences, Keio University (JP)
  • Nara Institute of Science and Technology (JP)
  • National Yang Ming University (TW)
  • Kyoto University (JP)
Venue
Genome Research
Published
2006-04-10
Volume
16
Issue
5
Pages
686-691
ISSN
1088-9051 (ISSN-L)
OpenAlex
W2137012036
Cited by
403
References
44
Citations by year
2026: 4 · 2025: 7 · 2024: 12 · 2023: 9 · 2022: 11 · 2021: 3 · 2020: 16 · 2019: 15 · 2018: 12 · 2017: 12 · 2016: 16 · 2015: 23 · 2014: 22 · 2013: 21 · 2012: 36
Type
posted content
Language
en
Open access
Locations
  • Genome Research (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed (publishedVersion)
Funders
Core Research for Evolutional Science and Technology
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioinformatics and Genomic Networks
  • Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
protein-protein interaction, Escherichia coli K-12, pull-down assay, protein-protein interaction network, interactome networks, protein function, systems biology, drug target identification
MeSH terms
Histidine, Models, Biological, Gene Library, Open Reading Frames, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Proteome, Escherichia coli Proteins, Proteomics, Escherichia coli K12

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review