A comprehensive two-hybrid analysis to explore the yeast protein interactome
Open accessAbstract
Protein–protein interactions play crucial roles in the execution of various biological functions. Accordingly, their comprehensive description would contribute considerably to the functional interpretation of fully sequenced genomes, which are flooded with novel genes of unpredictable functions. We previously developed a system to examine two-hybrid interactions in all possible combinations between the ≈6,000 proteins of the budding yeast Saccharomyces cerevisiae . Here we have completed the comprehensive analysis using this system to identify 4,549 two-hybrid interactions among 3,278 proteins. Unexpectedly, these data do not largely overlap with those obtained by the other project [Uetz, P., et al. (2000) Nature (London) 403, 623–627] and hence have substantially expanded our knowledge on the protein interaction space or interactome of the yeast. Cumulative connection of these binary interactions generates a single huge network linking the vast majority of the proteins. Bioinformatics-aided selection of biologically relevant interactions highlights various intriguing subnetworks. They include, for instance, the one that had successfully foreseen the involvement of a novel protein in spindle pole body function as well as the one that may uncover a hitherto unidentified multiprotein complex potentially participating in the process of vesicular transport. Our data would thus significantly expand and improve the protein interaction map for the exploration of genome functions that eventually leads to thorough understanding of the cell as a molecular system.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Y2H, APMS, and other high-throughput experimental approaches have identified many pairs of interacting proteins in yeast and other organisms, but there are discrepancies between sets generated using different methods and considerable false positive and false negative rates.
“Yeast two hybrid (Y2H), affinity-purification mass spectrometry (APMS), and other high-throughput experimental approaches have identified many pairs of interacting proteins in yeast and other organisms (1–5), but there are discrepancies between sets generated using the different methods and considerable false positive and false negative rates (6–8).”
From Computed structures of core eukaryotic protein complexes (2021) · cites this paper as [1]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
KEGG: Kyoto Encyclopedia of Genes and Genomes
Kanehisa, M. (2000) · Nucleic Acids Research · doi:10.1093/nar/28.1.27 · Cited by 37,077 · see citing works
KEGG: Kyoto Encyclopedia of Genes and Genomes
Ogata, H., Goto, S., Sato, K., Fujibuchi, W., Bono, H., & Kanehisa, M. (1999) · Nucleic Acids Research · doi:10.1093/nar/27.1.29 · Cited by 31,232 · see citing works
The complete genome sequence of Escherichia coli K-12.
Blattner, F., Plunkett, G., Bloch, C., Perna, N., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C., Mayhew, G., et al. (1997) · Science · doi:10.1126/science.277.5331.1453 · Cited by 7,996 · see citing works
A novel genetic system to detect protein–protein interactions
Fields, S., & Song, O.-K. (1989) · Nature · doi:10.1038/340245a0 · Cited by 6,152 · see citing works
A comprehensive analysis of protein–protein interactions in Saccharomyces cerevisiae
Uetz, P., Giot, L., Cagney, G., Mansfield, T. A., Judson, R. S., Knight, J. R., Lockshon, D., Narayan, V., Srinivasan, M., Pochart, P., et al. (2000) · Nature · doi:10.1038/35001009 · Cited by 4,706 · see citing works
Goffeau, A., Barrell, B., Bussey, H., Davis, R. W., Dujon, B., Feldmann, H., Galibert, F., Hoheisel, J., Jacq, C., Johnston, M., et al. (1996) · Science · doi:10.1126/science.274.5287.546 · Cited by 4,420 · see citing works
Winzeler, E. A., Shoemaker, D. D., Astromoff, A., Liang, H., Anderson, K., Andre, B., Bangham, R., Benito, R., Boeke, J. D., Bussey, H., et al. (1999) · Science · doi:10.1126/science.285.5429.901 · Cited by 3,953 · see citing works
Printing Proteins as Microarrays for High-Throughput Function Determination
MacBeath, G., & Schreiber, S. L. (2000) · Science · doi:10.1126/science.289.5485.1760 · Cited by 2,767 · see citing works
Genomic Libraries and a Host Strain Designed for Highly Efficient Two-Hybrid Selection in Yeast
James, P. M., Halladay, J. T., & Craig, E. A. (1996) · Genetics · doi:10.1093/genetics/144.4.1425 · Cited by 2,638 · see citing works
Proteomics to study genes and genomes
Pandey, A., & Mann, M. (2000) · Nature · doi:10.1038/35015709 · Cited by 2,373 · see citing works
Genomics, gene expression and DNA arrays
Lockhart, D. J., & Winzeler, E. A. (2000) · Nature · doi:10.1038/35015701 · Cited by 2,055 · see citing works
Functional genomic analysis of C. elegans chromosome I by systematic RNA interference
Fraser, A. G., Kamath, R. S., Zipperlen, P., Martinez-Campos, M., Sohrmann, M., & Ahringer, J. (2000) · Nature · doi:10.1038/35042517 · Cited by 1,720 · see citing works
A network of protein–protein interactions in yeast
Schwikowski, B., Uetz, P. H., & Fields, S. (2000) · Nature Biotechnology · doi:10.1038/82360 · Cited by 1,371 · see citing works
DIP: the Database of Interacting Proteins
Xénarios, I. (2000) · Nucleic Acids Research · doi:10.1093/nar/28.1.289 · Cited by 1,125 · see citing works
Toward a functional analysis of the yeast genome through exhaustive two-hybrid screens
Fromont‐Racine, M., Rain, J., & Legrain, P. (1997) · Nature Genetics · doi:10.1038/ng0797-277 · Cited by 913 · see citing works
Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III
Gönczy, P., Echeverri, C. J., Oegema, K., Coulson, A. R., Jones, S. J. M., Copley, R. R., Duperon, J., Oegema, J., Brehm, M., Cassin, E., et al. (2000) · Nature · doi:10.1038/35042526 · Cited by 858 · see citing works
Ito, T., Tashiro, K., Muta, S., Ozawa, R., Chiba, T., Nishizawa, M., Yamamoto, K., Kuhara, S., & Sakaki, Y. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.3.1143 · Cited by 814 · see citing works
Self-citationProtein Interaction Mapping in C. elegans Using Proteins Involved in Vulval Development
Walhout, A. J. M., Sordella, R., Lu, X., Hartley, J. L., Temple, G. F., Brasch, M. A., Thierry‐Mieg, N., & Vidal, M. (2000) · Science · doi:10.1126/science.287.5450.116 · Cited by 757 · see citing works
Vacuolar Import of Proteins and Organelles From The Cytoplasm
Klionsky, D. J., & Ohsumi, Y. (1999) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.15.1.1 · Cited by 467 · see citing works
Meyer, H. H., Shorter, J., Seemann, J., Pappin, D., & Warren, G. (2000) · The EMBO Journal · doi:10.1093/emboj/19.10.2181 · Cited by 446 · see citing works
Vidal, M., Brachmann, R. K., Fattaey, A., Harlow, E., & Boeke, J. D. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.19.10315 · Cited by 428 · see citing works
Costanzo, M. C. (2000) · Nucleic Acids Research · doi:10.1093/nar/28.1.73 · Cited by 288 · see citing works
A computationally directed screen identifying interacting coiled coils from Saccharomyces cerevisiae
Newman, J. R., Wolf, E., & Kim, P. S. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.24.13203 · Cited by 148 · see citing works
Genome‐wide protein interaction maps using two‐hybrid systems
Legrain, P., & Selig, L. (2000) · FEBS Letters · doi:10.1016/s0014-5793(00)01774-9 · Cited by 148 · see citing works
Systematic and large-scale two-hybrid screens
Uetz, P. H. (2000) · Current Opinion in Microbiology · doi:10.1016/s1369-5274(00)00094-1 · Cited by 138 · see citing works
Matern, H. T., Yang, X., Andrulis, E. D., et al. (2000) · The EMBO Journal · doi:10.1093/emboj/19.17.4485 · Cited by 99 · see citing works
Fashena, S. J., Serebriiskii, I. G., & Golemis, E. A. (2000) · Gene · doi:10.1016/s0378-1119(00)00182-7 · Cited by 68 · see citing works
Genome-Wide Protein Interaction Screens Reveal Functional Networks Involving Sm-Like Proteins
Fromont‐Racine, M., Mayes, A. E., Simon, A., et al. (2000) · Yeast · doi:10.1155/2000/919260 · Cited by 54 · see citing works
Integrative analysis of protein interaction data.
Fellenberg, M., Albermann, K., Zollner, A., et al. (2000) · PubMed · Cited by 46 · see citing works
Systematic function analysis of genes
Ogasawara, N. (2000) · Research in Microbiology · doi:10.1016/s0923-2508(00)00118-2 · Cited by 41 · see citing works
Yeast Two-Hybrid Systems and Protein Interaction Mapping Projects for Yeast and Worm
Walhout, A. J. M., Boulton, S. J., & Vidal, M. (2000) · Yeast · doi:10.1155/2000/156745 · Cited by 31 · see citing works
A guided tour in protein interaction space: Coiled coils from the yeast proteome
Hu, J. C. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.24.12935 · Cited by 13 · see citing works
Genomes 2000 International Conference on Microbial and Model Genomes
Wixon, J. (2000) · Yeast · doi:10.1002/1097-0061(20000630)17:2 · Cited by 3 · see citing works
Flagged first, then by citations
Research works printed but not linked: 5
- [10]Ito, T., Tashiro, K., Muta, S., Ozawa, R., Chiba, T., Nishizawa, M., Yamamoto, K., Kuhara, S. & Sakaki, Y. (2000) Proc. Natl. Acad. Sci. USA 97, 1143-1147.
- [13]Vidal, M., Brachmann, R. K., Fattaey, A., Harlow, E. & Boeke, J. (1996) Proc. Natl. Acad. Sci. USA 93, 10315-10320.
- [17]Yoshida, M., Fukagawa, H., Shibagaki, Y., Shimano, H. & Mizuno, T. (2000) Genome Inform. Ser. Workshop Genome Inform. 11, 364-365.
- [27]Hu, J. C. (2000) Proc. Natl. Acad. Sci. USA 97, 12935-12936.
- [28]Newman, J. R. S., Wolf, E. & Kim, P. S. (2000) Proc. Natl. Acad. Sci. USA 97, 13203-13208.
Cited by
Sorted by citations
Network biology: understanding the cell's functional organization
Barabási, A.-L., & Oltvai, Z. N. (2004) · Nature Reviews Genetics · doi:10.1038/nrg1272 · Cited by 7,903 · see citing works
Global analysis of protein localization in budding yeast
Huh, W.-K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., & O'Shea, E. K. (2003) · Nature · doi:10.1038/nature02026 · Cited by 4,248 · see citing works
BioGRID: a general repository for interaction datasets
Stark, C. (2006) · Nucleic Acids Research · doi:10.1093/nar/gkj109 · Cited by 3,960 · see citing works
The Database of Interacting Proteins: 2004 update
Salwinski, L. (2004) · Nucleic Acids Research · doi:10.1093/nar/gkh086 · Cited by 2,271 · see citing works
Hierarchical structure and the prediction of missing links in networks
Clauset, A., Moore, C., & Newman, M. E. J. (2008) · Nature · doi:10.1038/nature06830 · Cited by 2,171 · see citing works
Interactome Networks and Human Disease
Vidal, M., Cusick, M. E., & Barabási, A.-L. (2011) · Cell · doi:10.1016/j.cell.2011.02.016 · Cited by 1,771 · see citing works
Protein Analysis by Shotgun/Bottom-up Proteomics
Zhang, Y., Fonslow, B. R., Shan, B., Baek, M.-C., & Yates, J. R. (2013) · Chemical Reviews · doi:10.1021/cr3003533 · Cited by 1,491 · see citing works
High-Quality Binary Protein Interaction Map of the Yeast Interactome Network
Yu, H., Braun, P., Yıldırım, M. A., Lemmens, I., Venkatesan, K., Sahalie, J., Hirozane-Kishikawa, T., Gebreab, F., Li, N., Simonis, N., et al. (2008) · Science · doi:10.1126/science.1158684 · Cited by 1,392 · see citing works
P bodies and the control of mRNA translation and degradation
Parker, R., & Sheth, U. (2007) · Molecular Cell · doi:10.1016/j.molcel.2007.02.011 · Cited by 1,335 · see citing works
Cell Wall Integrity Signaling in <i>Saccharomyces cerevisiae</i>
Levin, D. E. (2005) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.69.2.262-291.2005 · Cited by 1,136 · see citing works
Flexible nets: The roles of intrinsic disorder in protein interaction networks
Dunker, A. K., Cortese, M. S., Romero, P., Iakoucheva, L. M., & Uversky, V. N. (2005) · The FEBS Journal · doi:10.1111/j.1742-4658.2005.04948.x · Cited by 1,122 · see citing works
Genome-Scale Reconstruction of the <i>Saccharomyces cerevisiae</i> Metabolic Network
Förster, J., Famili, I., Fu, P., Palsson, B. Ø., & Nielsen, J. (2003) · Genome Research · doi:10.1101/gr.234503 · Cited by 1,069 · see citing works
Schuldiner, M., Collins, S. R., Thompson, N. J., Denic, V., Bhamidipati, A., Punna, T., Ihmels, J., Andrews, B., Boone, C., Greenblatt, J. F., et al. (2005) · Cell · doi:10.1016/j.cell.2005.08.031 · Cited by 870 · see citing works
Zhao, R., Davey, M., Hsu, Y.-C., Kaplanek, P., Tong, A., Parsons, A. B., Krogan, N., Cagney, G., Mai, D., Greenblatt, J., et al. (2005) · Cell · doi:10.1016/j.cell.2004.12.024 · Cited by 819 · see citing works
Protein-Protein Interaction Detection: Methods and Analysis
Rao, V. S., Srinivas, K., Sujini, G. N., & Kumar, G. N. S. (2014) · International Journal of Proteomics · doi:10.1155/2014/147648 · Cited by 811 · see citing works
Ideker, T., & Sharan, R. (2008) · Genome Research · doi:10.1101/gr.071852.107 · Cited by 817 · see citing works
Toward a Comprehensive Atlas of the Physical Interactome of Saccharomyces cerevisiae
Collins, S. R., Kemmeren, P., Zhao, X.-C., Greenblatt, J. F., Spencer, F., Holstege, F. C. P., Weissman, J. S., & Krogan, N. J. (2007) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m600381-mcp200 · Cited by 795 · see citing works
Lim, J., Hao, T., Shaw, C., Patel, A. J., Szabó, G., Rual, J.-F., Fisk, C. J., Li, N., Smolyar, A., Hill, D. E., et al. (2006) · Cell · doi:10.1016/j.cell.2006.03.032 · Cited by 789 · see citing works
Phosphotyrosine interactome of the ErbB‐receptor kinase family
Schulze, W. X., Deng, L., & Mann, M. (2005) · Molecular Systems Biology · doi:10.1038/msb4100012 · Cited by 753 · see citing works
CAPRI: A Critical Assessment of PRedicted Interactions
Janin, J., Henrick, K., Moult, J., Eyck, L. T., Sternberg, M. J. E., Vajda, S., Vakser, I., & Wodak, S. J. (2003) · Proteins: Structure, Function, and Bioinformatics · doi:10.1002/prot.10381 · Cited by 703 · see citing works
Lipinski, M. M., Zheng, B., Lu, T., Yan, Z., Py, B. F., Ng, A., Xavier, R. J., Li, C., Yankner, B. A., Scherzer, C. R., et al. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1009485107 · Cited by 680 · see citing works
Exploring genetic interactions and networks with yeast
Boone, C., Bussey, H., & Andrews, B. J. (2007) · Nature Reviews Genetics · doi:10.1038/nrg2085 · Cited by 661 · see citing works
Exploration of Essential Gene Functions via Titratable Promoter Alleles
Mnaimneh, S., Davierwala, A. P., Haynes, J., Moffat, J., Peng, W.-T., Zhang, W., Yang, X., Pootoolal, J., Chua, G., Lopez, A., et al. (2004) · Cell · doi:10.1016/j.cell.2004.06.013 · Cited by 604 · see citing works
Computed structures of core eukaryotic protein complexes
Humphreys, I. R., Pei, J., Baek, M., Krishnakumar, A., Anishchenko, I., Ovchinnikov, S., Zhang, J., Ness, T. J., Banjade, S., Bagde, S. R., et al. (2021) · Science · doi:10.1126/science.abm4805 · Cited by 542 · see citing works
Integrative approaches for finding modular structure in biological networks
Mitra, K., Carvunis, A.-R., Ramesh, S. K., & Ideker, T. (2013) · Nature Reviews Genetics · doi:10.1038/nrg3552 · Cited by 535 · see citing works
Comprehensive Identification of Human bZIP Interactions with Coiled-Coil Arrays
Newman, J. R. S., & Keating, A. E. (2003) · Science · doi:10.1126/science.1084648 · Cited by 449 · see citing works
Cell-cell communication: New insights and clinical implications
Su, J., Song, Y., Zhu, Z., Huang, X., Fan, J., Qiao, J., & Mao, F. (2024) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-024-01888-z · Cited by 433 · see citing works
Large-scale identification of protein–protein interaction of <i>Escherichia coli</i> K-12
Arifuzzaman, M., Maeda, M., Itoh, A., Nishikata, K., Takita, C., Saito, R., Ara, T., Nakahigashi, K., Huang, H.-C., Hirai, A., et al. (2006) · Genome Research · doi:10.1101/gr.4527806 · Cited by 403 · see citing works
Deep learning in bioinformatics: Introduction, application, and perspective in the big data era
Li, Y., Huang, C., Ding, L., Li, Z., Pan, Y., & Gao, X. (2019) · Methods · doi:10.1016/j.ymeth.2019.04.008 · Cited by 401 · see citing works
Predicting Protein–Protein Interactions from the Molecular to the Proteome Level
Keskin, O., Tuncbag, N., & Gursoy, A. (2016) · Chemical Reviews · doi:10.1021/acs.chemrev.5b00683 · Cited by 369 · see citing works
Systematic Discovery of New Recognition Peptides Mediating Protein Interaction Networks
Neduva, V., Linding, R., Su-Angrand, I., Stark, A., Masi, F. D., Gibson, T. J., Lewis, J., Serrano, L., & Russell, R. B. (2005) · PLoS Biology · doi:10.1371/journal.pbio.0030405 · Cited by 359 · see citing works
Comprehensive curation and analysis of global interaction networks in Saccharomyces cerevisiae
Reguly, T., Breitkreutz, A., Boucher, L., Breitkreutz, B.-J., Hon, G. C., Myers, C. L., Parsons, A., Friesen, H., Oughtred, R., Tong, A., et al. (2006) · Journal of Biology · doi:10.1186/jbiol36 · Cited by 326 · see citing works
Bridging structural biology and genomics: assessing protein interaction data with known complexes
Edwards, A. M., Kus, B., Jansen, R., Greenbaum, D., Greenblatt, J., & Gerstein, M. (2002) · Trends in Genetics · doi:10.1016/s0168-9525(02)02763-4 · Cited by 276 · see citing works
Trans-Omics: How To Reconstruct Biochemical Networks Across Multiple ‘Omic’ Layers
Yugi, K., Kubota, H., Hatano, A., & Kuroda, S. (2016) · Trends in Biotechnology · doi:10.1016/j.tibtech.2015.12.013 · Cited by 258 · see citing works
Pijnappel, W. W. M. P., Schaft, D., Roguev, A., Shevchenko, A., Tekotte, H., Wilm, M., Rigaut, G., Séraphin, B., Aasland, R., & Stewart, A. F. (2001) · Genes & Development · doi:10.1101/gad.207401 · Cited by 265 · see citing works
Combining biological networks to predict genetic interactions
Wong, S. L., Zhang, L. V., Tong, A. H. Y., Li, Z., Goldberg, D. S., King, O. D., Lesage, G., Vidal, M., Andrews, B., Bussey, H., et al. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0406614101 · Cited by 245 · see citing works
Probabilistic assembly of human protein interaction networks from label-free quantitative proteomics
Sardiu, M. E., Cai, Y., Jin, J., Swanson, S. K., Conaway, R. C., Conaway, J. W., Florens, L., & Washburn, M. P. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0706983105 · Cited by 226 · see citing works
Mansfeld, J., Güttinger, S., Hawryluk-Gara, L. A., Panté, N., Mall, M., Galy, V., Haselmann, U., Mühlhäusser, P., Wozniak, R. W., Mattaj, I. W., et al. (2006) · Molecular Cell · doi:10.1016/j.molcel.2006.02.015 · Cited by 221 · see citing works
Integrating physical and genetic maps: from genomes to interaction networks
Beyer, A., Bandyopadhyay, S., & Ideker, T. (2007) · Nature Reviews Genetics · doi:10.1038/nrg2144 · Cited by 207 · see citing works
Protein-protein interaction networks and biology—what's the connection?
Hakes, L., Pinney, J. W., Robertson, D. L., & Lovell, S. C. (2008) · Nature Biotechnology · doi:10.1038/nbt0108-69 · Cited by 200 · see citing works
Bhardwaj, N., & Lu, H. (2005) · Bioinformatics · doi:10.1093/bioinformatics/bti398 · Cited by 186 · see citing works
Where Have All the Interactions Gone? Estimating the Coverage of Two-Hybrid Protein Interaction Maps
Huang, H., Jedynak, B. M., & Bader, J. S. (2007) · PLoS Computational Biology · doi:10.1371/journal.pcbi.0030214 · Cited by 182 · see citing works
TRAM, LAG1 and CLN8: members of a novel family of lipid-sensing domains?
Winter, E., & Ponting, C. P. (2002) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(02)02154-0 · Cited by 181 · see citing works
Biochemical approaches for discovering protein–protein interactions
Miernyk, J. A., & Thelen, J. J. (2008) · The Plant Journal · doi:10.1111/j.1365-313x.2007.03316.x · Cited by 165 · see citing works
Mechanisms of replication fork protection: a safeguard for genome stability
Errico, A., & Costanzo, V. (2012) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.3109/10409238.2012.655374 · Cited by 159 · see citing works
Molecular architecture of the yeast Mediator complex
Robinson, P. J., Trnka, M. J., Pellarin, R., Greenberg, C. H., Bushnell, D. A., Davis, R., Burlingame, A. L., Sali, A., & Kornberg, R. D. (2015) · eLife · doi:10.7554/elife.08719 · Cited by 150 · see citing works
Identification of Protein Interactions Involved in Cellular Signaling
Westermarck, J., Ivaska, J., & Corthals, G. L. (2013) · Molecular & Cellular Proteomics · doi:10.1074/mcp.r113.027771 · Cited by 143 · see citing works
Tessé, S., Storlazzi, A., Kleckner, N., Gargano, S., & Zickler, D. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2034282100 · Cited by 132 · see citing works
Robotic cloning and protein production platform of the northeast structural genomics consortium
Acton, T. B., Gunsalus, K. C., Xiao, R., Ma, L. C., Aramini, J., Baran, M. C., Chiang, Y.-W., Climent, T., Cooper, B., Denissova, N. G., et al. (2005) · Methods in Enzymology · doi:10.1016/s0076-6879(05)94008-1 · Cited by 129 · see citing works
Flott, S., Alabert, C., Toh, G. W., Toth, R., Sugawara, N., Campbell, D. G., Haber, J. E., Pasero, P., & Rouse, J. (2007) · Molecular and Cellular Biology · doi:10.1128/mcb.00135-07 · Cited by 107 · see citing works
Errico, A., Costanzo, V., & Hunt, T. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0706347104 · Cited by 92 · see citing works
A map of human protein interactions derived from co‐expression of human mRNAs and their orthologs
Ramani, A. K., Li, Z., Hart, G. T., Carlson, M. W., Boutz, D. R., & Marcotte, E. M. (2008) · Molecular Systems Biology · doi:10.1038/msb.2008.19 · Cited by 78 · see citing works
Cornet, M., Bidard, F., Schwarz, P., Da Costa, G., Blanchin-Roland, S., Dromer, F., & Gaillardin, C. (2005) · Infection and Immunity · doi:10.1128/iai.73.12.7977-7987.2005 · Cited by 70 · see citing works
Integrative structure modeling of macromolecular assemblies from proteomics data
Lasker, K., Phillips, J. L., Russel, D., Velázquez-Muriel, J., Schneidman-Duhovny, D., Tjioe, E., Webb, B., Schlessinger, A., & Sali, A. (2010) · Molecular & Cellular Proteomics · doi:10.1074/mcp.r110.000067 · Cited by 71 · see citing works
Temporal patterns of genes in scientific publications
Pfeiffer, T., & Hoffmann, R. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0701315104 · Cited by 64 · see citing works
M-TASSER: An Algorithm for Protein Quaternary Structure Prediction
Chen, H., & Skolnick, J. (2008) · Biophysical Journal · doi:10.1529/biophysj.107.114280 · Cited by 68 · see citing works
Rossi, L., Torsello, A., & Hancock, E. R. (2015) · Physical Review E · doi:10.1103/physreve.91.022815 · Cited by 46 · see citing works
Higher-order molecular organization as a source of biological function
Gaudelet, T., Malod-Dognin, N., & Pržulj, N. (2018) · Bioinformatics · doi:10.1093/bioinformatics/bty570 · Cited by 46 · see citing works
Suzuki, Y., Murray, S. L., Wong, K. H., Davis, M. A., & Hynes, M. J. (2012) · Molecular Microbiology · doi:10.1111/j.1365-2958.2012.08067.x · Cited by 46 · see citing works
Van HOOF, C., MARTENS, E., LONGIN, S., JORDENS, J., STEVENS, I., JANSSENS, V., & GORIS, J. (2005) · Biochemical Journal · doi:10.1042/bj20040887 · Cited by 42 · see citing works
Gingras, A.-C., & Raught, B. (2012) · FEBS Letters · doi:10.1016/j.febslet.2012.03.065 · Cited by 34 · see citing works
Janin, J., & Wodak, S. J. (2002) · Advances in Protein Chemistry · doi:10.1016/s0065-3233(02)61000-9 · Cited by 31 · see citing works
<i>Candida glabrata</i> : new tools and technologies—expanding the toolkit
Ho, H.-L., & Haynes, K. (2015) · FEMS Yeast Research · doi:10.1093/femsyr/fov066 · Cited by 29 · see citing works
Lst4, the yeast Fnip1/2 orthologue, is a DENN-family protein
Pacitto, A., Ascher, D. B., Wong, L. H., Blaszczyk, B. K., Nookala, R. K., Zhang, N., Dokudovskaya, S., Levine, T. P., & Blundell, T. L. (2015) · Open Biology · doi:10.1098/rsob.150174 · Cited by 28 · see citing works
Chae, M. S., Nargang, C. E., Cleary, I. A., Lin, C. C., Todd, A. T., & Nargang, F. E. (2007) · Genetics · doi:10.1534/genetics.107.078212 · Cited by 22 · see citing works
Chae, M. S., & Nargang, F. E. (2009) · Physiologia Plantarum · doi:10.1111/j.1399-3054.2009.01239.x · Cited by 17 · see citing works
Network-Free Inference of Knockout Effects in Yeast
Peleg, T., Yosef, N., Ruppin, E., & Sharan, R. (2010) · PLoS Computational Biology · doi:10.1371/journal.pcbi.1000635 · Cited by 14 · see citing works
Rosenkrantz, J. T., Aarts, H., Abee, T., Rolfe, M. D., Knudsen, G. M., Nielsen, M.-B., Thomsen, L. E., Zwietering, M. H., Olsen, J. E., & Pin, C. (2013) · BMC Microbiology · doi:10.1186/1471-2180-13-294 · Cited by 13 · see citing works
- Authors
- Ito, T., Chiba, T., Ozawa, R., Yoshida, M., Hattori, M., & Sakaki, Y.
- Affiliations
- Kanazawa University (JP)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2001-03-13
- Volume
- 98
- Issue
- 8
- Pages
- 4569-4574
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC31875
- OpenAlex
- W2167869241
- Cited by
- 3,566
- References
- 33
- Citations by year
- 2026: 25 · 2025: 34 · 2024: 46 · 2023: 38 · 2022: 52 · 2021: 49 · 2020: 55 · 2019: 65 · 2018: 74 · 2017: 67 · 2016: 81 · 2015: 111 · 2014: 98 · 2013: 164 · 2012: 178
- FWCI
- 82.80
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioinformatics and Genomic Networks
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Biotin and Related Studies
- Keywords
- protein-protein interaction, protein-protein interaction network, Saccharomyces cerevisiae, two-hybrid screen, interactome mapping, spindle pole body, vesicle trafficking, protein complexes
- MeSH terms
- Fungal Proteins, Protein Binding, Saccharomyces cerevisiae, Genome, Fungal, Two-Hybrid System Techniques
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review