Cited by 3,566Bioinformatics and Genomic NetworksFungal and yeast genetics researchBiotin and Related Studies

A comprehensive two-hybrid analysis to explore the yeast protein interactome

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Ito, Takashi, Chiba, Tomoko, Ozawa, Ritsuko, Yoshida, Mikio, Hattori, Masahira, Sakaki, Yoshiyuki·2001·Proceedings of the National Academy of Sciences

Abstract

Protein–protein interactions play crucial roles in the execution of various biological functions. Accordingly, their comprehensive description would contribute considerably to the functional interpretation of fully sequenced genomes, which are flooded with novel genes of unpredictable functions. We previously developed a system to examine two-hybrid interactions in all possible combinations between the ≈6,000 proteins of the budding yeast Saccharomyces cerevisiae . Here we have completed the comprehensive analysis using this system to identify 4,549 two-hybrid interactions among 3,278 proteins. Unexpectedly, these data do not largely overlap with those obtained by the other project [Uetz, P., et al. (2000) Nature (London) 403, 623–627] and hence have substantially expanded our knowledge on the protein interaction space or interactome of the yeast. Cumulative connection of these binary interactions generates a single huge network linking the vast majority of the proteins. Bioinformatics-aided selection of biologically relevant interactions highlights various intriguing subnetworks. They include, for instance, the one that had successfully foreseen the involvement of a novel protein in spindle pole body function as well as the one that may uncover a hitherto unidentified multiprotein complex potentially participating in the process of vesicular transport. Our data would thus significantly expand and improve the protein interaction map for the exploration of genome functions that eventually leads to thorough understanding of the cell as a molecular system.

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  • Y2H, APMS, and other high-throughput experimental approaches have identified many pairs of interacting proteins in yeast and other organisms, but there are discrepancies between sets generated using different methods and considerable false positive and false negative rates.

    “Yeast two hybrid (Y2H), affinity-purification mass spectrometry (APMS), and other high-throughput experimental approaches have identified many pairs of interacting proteins in yeast and other organisms (1–5), but there are discrepancies between sets generated using the different methods and considerable false positive and false negative rates (6–8).”

    From Computed structures of core eukaryotic protein complexes (2021) · cites this paper as [1]

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  1. [10]Ito, T., Tashiro, K., Muta, S., Ozawa, R., Chiba, T., Nishizawa, M., Yamamoto, K., Kuhara, S. & Sakaki, Y. (2000) Proc. Natl. Acad. Sci. USA 97, 1143-1147.
  2. [13]Vidal, M., Brachmann, R. K., Fattaey, A., Harlow, E. & Boeke, J. (1996) Proc. Natl. Acad. Sci. USA 93, 10315-10320.
  3. [17]Yoshida, M., Fukagawa, H., Shibagaki, Y., Shimano, H. & Mizuno, T. (2000) Genome Inform. Ser. Workshop Genome Inform. 11, 364-365.
  4. [27]Hu, J. C. (2000) Proc. Natl. Acad. Sci. USA 97, 12935-12936.
  5. [28]Newman, J. R. S., Wolf, E. & Kim, P. S. (2000) Proc. Natl. Acad. Sci. USA 97, 13203-13208.

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Authors
Ito, T., Chiba, T., Ozawa, R., Yoshida, M., Hattori, M., & Sakaki, Y.
Affiliations
Kanazawa University (JP)
Venue
Proceedings of the National Academy of Sciences
Published
2001-03-13
Volume
98
Issue
8
Pages
4569-4574
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2167869241
Cited by
3,566
References
33
Citations by year
2026: 25 · 2025: 34 · 2024: 46 · 2023: 38 · 2022: 52 · 2021: 49 · 2020: 55 · 2019: 65 · 2018: 74 · 2017: 67 · 2016: 81 · 2015: 111 · 2014: 98 · 2013: 164 · 2012: 178
FWCI
82.80
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioinformatics and Genomic Networks
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Biotin and Related Studies
Keywords
protein-protein interaction, protein-protein interaction network, Saccharomyces cerevisiae, two-hybrid screen, interactome mapping, spindle pole body, vesicle trafficking, protein complexes
MeSH terms
Fungal Proteins, Protein Binding, Saccharomyces cerevisiae, Genome, Fungal, Two-Hybrid System Techniques

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