The DOI matches its Crossref record.
CYP71B15 (PAD3) Catalyzes the Final Step in Camalexin Biosynthesis
Open accessAbstract
Camalexin represents the main phytoalexin in Arabidopsis (Arabidopsis thaliana). The camalexin-deficient phytoalexin deficient 3 (pad3) mutant has been widely used to assess the biological role of camalexin, although the exact substrate of the cytochrome P450 enzyme 71B15 encoded by PAD3 remained elusive. 2-(Indol-3-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid (dihydrocamalexic acid) was identified as likely intermediate in camalexin biosynthesis downstream of indole-3-acetaldoxime, as it accumulated in leaves of silver nitrate-induced pad3 mutant plants and it complemented the camalexin-deficient phenotype of a cyp79b2/cyp79b3 double-knockout mutant. Recombinant CYP71B15 heterologously expressed in yeast catalyzed the conversion of dihydrocamalexic acid to camalexin with preference of the (S)-enantiomer. Arabidopsis microsomes isolated from leaves of CYP71B15-overexpressing and induced wild-type plants were capable of the same reaction but not microsomes from induced leaves of pad3 mutants. In conclusion, CYP71B15 catalyzes the final step in camalexin biosynthesis.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Glucosinolate Metabolites Required for an Arabidopsis Innate Immune Response
Clay, N. K., Adio, A. M., Denoux, C., Jander, G., & Ausubel, F. M. (2009) · Science · doi:10.1126/science.1164627 · Cited by 1,098
Indole Glucosinolate Biosynthesis Limits Phenylpropanoid Accumulation in Arabidopsis thaliana
Kim, J. I., Dolan, W. L., Anderson, N. A., & Chapple, C. (2015) · The Plant Cell · doi:10.1105/tpc.15.00127 · Cited by 117
Metadata
- Authors
- Schuhegger, R., Nafisi, M., Mansourova, M., Petersen, B. L., Olsen, C. E., Svatoš, A., Halkier, B. A., & Glawischnig, E.
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2006-06-09
- Volume
- 141
- Issue
- 4
- Pages
- 1248-1254
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- OpenAlex
- W2169346822
- Cited by
- 277
- References
- 0
- FWCI
- 6.63
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: CYP71B15 (PAD3) Catalyzes the Final Step in Camalexin Biosynthesis. https://app.cochat.ai/papers/10.1104/pp.106.082024
- The paper
- Schuhegger, R., Nafisi, M., Mansourova, M., Petersen, B. L., Olsen, C. E., Svatoš, A., Halkier, B. A., & Glawischnig, E. (2006). CYP71B15 (PAD3) Catalyzes the Final Step in Camalexin Biosynthesis. Plant Physiology. https://doi.org/10.1104/pp.106.082024
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
