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IRT1, an Arabidopsis Transporter Essential for Iron Uptake from the Soil and for Plant Growth
Open accessAbstract
Plants are the principal source of iron in most diets, yet iron availability often limits plant growth. In response to iron deficiency, Arabidopsis roots induce the expression of the divalent cation transporter IRT1. Here, we present genetic evidence that IRT1 is essential for the uptake of iron from the soil. An Arabidopsis knockout mutant in IRT1 is chlorotic and has a severe growth defect in soil, leading to death. This defect is rescued by the exogenous application of iron. The mutant plants do not take up iron and fail to accumulate other divalent cations in low-iron conditions. IRT1-green fluorescent protein fusion, transiently expressed in culture cells, localized to the plasma membrane. We also show, through promoter::beta-glucuronidase analysis and in situ hybridization, that IRT1 is expressed in the external cell layers of the root, specifically in response to iron starvation. These results clearly demonstrate that IRT1 is the major transporter responsible for high-affinity metal uptake under iron deficiency.
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“For influx to the symplasm, a lot of proteins such as ZRT- and IRT-like proteins, stripe-Like (YSL) transporters, natural resistance-associated macrophage proteins (NRAMP), channels and other genes related to Cd-uptake/restriction play critical roles [32].”
From Enhanced tolerance of industrial hemp (Cannabis sativa L.) plants on abandoned mine land soil leads to overexpression of cannabinoids (2019) · cites this paper as [32]
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Metadata
- Authors
- Vert, G., Grotz, N., Dédaldéchamp, F., Gaymard, F., Guerinot, M. L., Briat, J.-F., & Curie, C.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Dartmouth College (US)
- ORCID
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 2002-06
- Volume
- 14
- Issue
- 6
- Pages
- 1223-1233
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- PMC
- PMC150776
- OpenAlex
- W2124946184
- Cited by
- 1,679
- References
- 42
- Citations by year
- 2026: 89 · 2025: 80 · 2024: 81 · 2023: 100 · 2022: 121 · 2021: 113 · 2020: 98 · 2019: 92 · 2018: 82 · 2017: 78 · 2016: 75 · 2015: 73 · 2014: 77 · 2013: 66 · 2012: 73
- FWCI
- 37.16
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Plant Cell (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Micronutrient Interactions and Effects
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
- Keywords
- IRT1, iron absorption, iron deficiency, Arabidopsis thaliana, plasma membrane localization, chlorosis, knockout mutants, iron starvation response
- MeSH terms
- Base Sequence, Biological Transport, Cations, Divalent, Genes, Lethal, Homeostasis, Iron, Luminescent Proteins, NADH, NADPH Oxidoreductases, Plant Proteins, Plasmids, Recombinant Fusion Proteins, Soil, Genes, Plant, Gene Deletion, Arabidopsis, DNA Primers, Plant Roots, Cation Transport Proteins, Arabidopsis Proteins, Plants, Genetically Modified, FMN Reductase, Green Fluorescent Proteins
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