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The Arabidopsis Mutant sleepy1gar2-1 Protein Promotes Plant Growth by Increasing the Affinity of the SCFSLY1 E3 Ubiquitin Ligase for DELLA Protein Substrates[W]
Open accessAbstract
DELLA proteins restrain the cell proliferation and enlargement that characterizes the growth of plant organs. Gibberellin stimulates growth via 26S proteasome–dependent destruction of DELLAs, thus relieving DELLA-mediated growth restraint. Here, we show that the Arabidopsis thaliana sleepy1gar2-1 (sly1gar2-1) mutant allele encodes a mutant subunit (sly1gar2-1) of an SCFSLY1 E3 ubiquitin ligase complex. SLY1 (the wild-type form) and sly1gar2-1 both confer substrate specificity on this complex via specific binding to the DELLA proteins. However, sly1gar2-1 interacts more strongly with the DELLA target than does SLY1. In addition, the strength of the SCFSLY1–DELLA interaction is increased by target phosphorylation. Growth-promoting DELLA destruction is dependent on SLY1 availability, on the strength of the interaction between SLY1 and the DELLA target, and on promotion of the SCFSLY1–DELLA interaction by DELLA phosphorylation.
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Cites
Structure of the Cul1–Rbx1–Skp1–F boxSkp2 SCF ubiquitin ligase complex
Zheng, N., Schulman, B. A., Song, L., Miller, J. J., Jeffrey, P. D., Wang, P., Chu, C., Koepp, D. M., Elledge, S. J., Pagano, M., et al. (2002) · Nature · doi:10.1038/416703a · Cited by 1,507
Degradation of Cdc25A by β-TrCP during S phase and in response to DNA damage
Busino, L., Donzelli, M., Chiesa, M., Guardavaccaro, D., Ganoth, D., Valerio Dorrello, N., Hershko, A., Pagano, M., & Draetta, G. F. (2003) · Nature · doi:10.1038/nature02082 · Cited by 447
Cited by
Molecular Aspects of Seed Dormancy
Finkelstein, R., Reeves, W., Ariizumi, T., & Steber, C. (2008) · Annual Review of Plant Biology · doi:10.1146/annurev.arplant.59.032607.092740 · Cited by 1,379
Global Analysis of DELLA Direct Targets in Early Gibberellin Signaling in Arabidopsis
Zentella, R., Zhang, Z.-L., Park, M., Thomas, S. G., Endo, A., Murase, K., Fleet, C. M., Jikumaru, Y., Nambara, E., Kamiya, Y., et al. (2007) · The Plant Cell · doi:10.1105/tpc.107.054999 · Cited by 678
Jiang, C., Gao, X., Liao, L., Harberd, N. P., & Fu, X. (2007) · Plant Physiology · doi:10.1104/pp.107.103788 · Cited by 466
Self-citationWhole-Genome Analysis of the SHORT-ROOT Developmental Pathway in Arabidopsis
Levesque, M. P., Vernoux, T., Busch, W., Cui, H., Wang, J. Y., Blilou, I., Hassan, H., Nakajima, K., Matsumoto, N., Lohmann, J. U., et al. (2006) · PLoS Biology · doi:10.1371/journal.pbio.0040143 · Cited by 349
An Updated Overview on the Regulation of Seed Germination
Carrera-Castaño, G., Calleja-Cabrera, J., Pernas, M., Gómez, L., & Oñate-Sánchez, L. (2020) · Plants · doi:10.3390/plants9060703 · Cited by 269
Metadata
- Authors
- Fu, X., Richards, D. E., Fleck, B., Xie, D., Burton, N., & Harberd, N. P.
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-06-01
- Volume
- 16
- Issue
- 6
- Pages
- 1406-1418
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- OpenAlex
- W2108097646
- Cited by
- 295
- References
- 2
- FWCI
- 20.24
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Fu, X., Richards, D. E., Fleck, B., Xie, D., Burton, N., & Harberd, N. P. (2004). The Arabidopsis Mutant sleepy1gar2-1 Protein Promotes Plant Growth by Increasing the Affinity of the SCFSLY1 E3 Ubiquitin Ligase for DELLA Protein Substrates[W]. The Plant Cell. https://doi.org/10.1105/tpc.021386
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