The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
GLYCOALKALOID METABOLISM1 Is Required for Steroidal Alkaloid Glycosylation and Prevention of Phytotoxicity in Tomato
Open accessAbstract
Steroidal alkaloids (SAs) are triterpene-derived specialized metabolites found in members of the Solanaceae family that provide plants with a chemical barrier against a broad range of pathogens. Their biosynthesis involves the action of glycosyltransferases to form steroidal glycoalkaloids (SGAs). To elucidate the metabolism of SGAs in the Solanaceae family, we examined the tomato (Solanum lycopersicum) GLYCOALKALOID METABOLISM1 (GAME1) gene. Our findings imply that GAME1 is a galactosyltransferase, largely performing glycosylation of the aglycone tomatidine, resulting in SGA production in green tissues. Downregulation of GAME1 resulted in an almost 50% reduction in α-tomatine levels (the major SGA in tomato) and a large increase in its precursors (i.e., tomatidenol and tomatidine). Surprisingly, GAME1-silenced plants displayed growth retardation and severe morphological phenotypes that we suggest occur as a result of altered membrane sterol levels caused by the accumulation of the aglycone tomatidine. Together, these findings highlight the role of GAME1 in the glycosylation of SAs and in reducing the toxicity of SA metabolites to the plant cell.
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Cites
Fungal Resistance to Plant Antibiotics as a Mechanism of Pathogenesis
Morrissey, J. P., & Osbourn, A. E. (1999) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.63.3.708-724.1999 · Cited by 635 · cited 4×
“Similar to other defensive secondarymetabolites, SAs are part of the coevolution between plants and pathogens (Morrissey and Osbourn, 1999; Arie et al., 2007).”
BackgroundInfluentialA saponin-detoxifying enzyme mediates suppression of plant defences
Bouarab, K., Melton, R., Peart, J., Baulcombe, D., & Osbourn, A. (2002) · Nature · doi:10.1038/nature00950 · Cited by 257
Cited by
Triterpene Biosynthesis in Plants
Thimmappa, R., Geisler, K., Louveau, T., O'Maille, P., & Osbourn, A. (2014) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-050312-120229 · Cited by 803
The Structure and Function of Major Plant Metabolite Modifications
Wang, S., Alseekh, S., Fernie, A. R., & Luo, J. (2019) · Molecular Plant · doi:10.1016/j.molp.2019.06.001 · Cited by 428
Personalized microbiome-driven effects of non-nutritive sweeteners on human glucose tolerance
Suez, J., Cohen, Y., Valdés-Mas, R., Mor, U., Dori-Bachash, M., Federici, S., Zmora, N., Leshem, A., Heinemann, M., Linevsky, R., et al. (2022) · Cell · doi:10.1016/j.cell.2022.07.016 · Cited by 423
Self-citationPlant chemical defense: at what cost?
Neilson, E. H., Goodger, J. Q. D., Woodrow, I. E., & Møller, B. L. (2013) · Trends in Plant Science · doi:10.1016/j.tplants.2013.01.001 · Cited by 385
Plant cholesterol biosynthetic pathway overlaps with phytosterol metabolism
Sonawane, P. D., Pollier, J., Panda, S., Szymanski, J., Massalha, H., Yona, M., Unger, T., Malitsky, S., Arendt, P., Pauwels, L., et al. (2016) · Nature Plants · doi:10.1038/nplants.2016.205 · Cited by 336
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Metadata
- Authors
- Itkin, M., Rogachev, I., Alkan, N., Rosenberg, T., Malitsky, S., Masini, L., Meir, S., Iijima, Y., Aoki, K., de Vos, R., Prusky, D., Burdman, S., Beekwilder, J., & Aharoni, A.
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-12-01
- Volume
- 23
- Issue
- 12
- Pages
- 4507-4525
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- OpenAlex
- W2166142631
- Cited by
- 253
- References
- 2
- FWCI
- 3.53
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: GLYCOALKALOID METABOLISM1 Is Required for Steroidal Alkaloid Glycosylation and Prevention of Phytotoxicity in Tomato. https://app.cochat.ai/papers/10.1105/tpc.111.088732
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- Itkin, M., Rogachev, I., Alkan, N., Rosenberg, T., Malitsky, S., Masini, L., Meir, S., Iijima, Y., Aoki, K., de Vos, R., Prusky, D., Burdman, S., Beekwilder, J., & Aharoni, A. (2011). GLYCOALKALOID METABOLISM1 Is Required for Steroidal Alkaloid Glycosylation and Prevention of Phytotoxicity in Tomato. The Plant Cell. https://doi.org/10.1105/tpc.111.088732
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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