The DOI matches its Crossref record; 84 of the 84 references it cites were found in a registry or an index.
Transcriptome Sequencing Identifies SPL7-Regulated Copper Acquisition Genes FRO4/FRO5 and the Copper Dependence of Iron Homeostasis in Arabidopsis
Open accessAbstract
The transition metal copper (Cu) is essential for all living organisms but is toxic when present in excess. To identify Cu deficiency responses comprehensively, we conducted genome-wide sequencing-based transcript profiling of Arabidopsis thaliana wild-type plants and of a mutant defective in the gene encoding SQUAMOSA PROMOTER BINDING PROTEIN-LIKE7 (SPL7), which acts as a transcriptional regulator of Cu deficiency responses. In response to Cu deficiency, FERRIC REDUCTASE OXIDASE5 (FRO5) and FRO4 transcript levels increased strongly, in an SPL7-dependent manner. Biochemical assays and confocal imaging of a Cu-specific fluorophore showed that high-affinity root Cu uptake requires prior FRO5/FRO4-dependent Cu(II)-specific reduction to Cu(I) and SPL7 function. Plant iron (Fe) deficiency markers were activated in Cu-deficient media, in which reduced growth of the spl7 mutant was partially rescued by Fe supplementation. Cultivation in Cu-deficient media caused a defect in root-to-shoot Fe translocation, which was exacerbated in spl7 and associated with a lack of ferroxidase activity. This is consistent with a possible role for a multicopper oxidase in Arabidopsis Fe homeostasis, as previously described in yeast, humans, and green algae. These insights into root Cu uptake and the interaction between Cu and Fe homeostasis will advance plant nutrition, crop breeding, and biogeochemical research.
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Cited by
Copper bioavailability, uptake, toxicity and tolerance in plants: A comprehensive review
Kumar, V., Pandita, S., Singh Sidhu, G. P., Sharma, A., Khanna, K., Kaur, P., Bali, A. S., & Setia, R. (2021) · Chemosphere · doi:10.1016/j.chemosphere.2020.127810 · Cited by 594
Molecular mechanisms governing Arabidopsis iron uptake
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IRON MAN is a ubiquitous family of peptides that control iron transport in plants
Grillet, L., Lan, P., Li, W., Mokkapati, G., & Schmidt, W. (2018) · Nature Plants · doi:10.1038/s41477-018-0266-y · Cited by 267
Copper and Iron Homeostasis in Plants: The Challenges of Oxidative Stress
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Metadata
- Authors
- Bernal, M., Casero, D., Singh, V., Wilson, G. T., Grande, A., Yang, H., Dodani, S. C., Pellegrini, M., Huijser, P., Connolly, E. L., Merchant, S. S., & Krämer, U.
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 2012-02
- Volume
- 24
- Issue
- 2
- Pages
- 738-761
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- OpenAlex
- W2128171377
- Cited by
- 323
- References
- 84
- FWCI
- 26.77
- Type
- journal article
- Language
- en
- Open access
- green
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- Bernal, M., Casero, D., Singh, V., Wilson, G. T., Grande, A., Yang, H., Dodani, S. C., Pellegrini, M., Huijser, P., Connolly, E. L., Merchant, S. S., & Krämer, U. (2012). Transcriptome Sequencing Identifies SPL7-Regulated Copper Acquisition Genes FRO4/FRO5 and the Copper Dependence of Iron Homeostasis in Arabidopsis. The Plant Cell. https://doi.org/10.1105/tpc.111.090431
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