The DOI matches its Crossref record; 65 of the 65 references it cites were found in a registry or an index.
Proteomics of the Chloroplast: Systematic Identification and Targeting Analysis of Lumenal and Peripheral Thylakoid Proteins
Open accessAbstract
The soluble and peripheral proteins in the thylakoids of pea were systematically analyzed by using two-dimensional electrophoresis, mass spectrometry, and N-terminal Edman sequencing, followed by database searching. After correcting to eliminate possible isoforms and post-translational modifications, we estimated that there are at least 200 to 230 different lumenal and peripheral proteins. Sixty-one proteins were identified; for 33 of these proteins, a clear function or functional domain could be identified, whereas for 10 proteins, no function could be assigned. For 18 proteins, no expressed sequence tag or full-length gene could be identified in the databases, despite experimental determination of a significant amount of amino acid sequence. Nine previously unidentified proteins with lumenal transit peptides are presented along with their full-length genes; seven of these proteins possess the twin arginine motif that is characteristic for substrates of the TAT pathway. Logoplots were used to provide a detailed analysis of the lumenal targeting signals, and all nuclear-encoded proteins identified on the two-dimensional gels were used to test predictions for chloroplast localization and transit peptides made by the software programs ChloroP, PSORT, and SignalP. A combination of these three programs was found to provide a useful tool for evaluating chloroplast localization and transit peptides and also could reveal possible alternative processing sites and dual targeting. The potential of proteomics for plant biology and homology-based searching with mass spectrometry data is discussed.
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Metadata
- Authors
- Peltier, J.-B., Friso, G., Kalume, D. E., Roepstorff, P., Nilsson, F., Adamska, I., & van Wijka, K. J.
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 2000-03
- Volume
- 12
- Issue
- 3
- Pages
- 319-341
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- OpenAlex
- W2134713413
- Cited by
- 355
- References
- 65
- FWCI
- 11.49
- Type
- journal article
- Language
- en
- Open access
- green
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- Peltier, J.-B., Friso, G., Kalume, D. E., Roepstorff, P., Nilsson, F., Adamska, I., & van Wijka, K. J. (2000). Proteomics of the Chloroplast: Systematic Identification and Targeting Analysis of Lumenal and Peripheral Thylakoid Proteins. The Plant Cell. https://doi.org/10.1105/tpc.12.3.319
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