Cited by 896Plant tissue culture and regenerationPlant Molecular Biology ResearchGenetically Modified Organisms Research

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Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation

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Lowe, Keith, Wu, Emily, Wang, Ning, Hoerster, George, Hastings, Craig, Cho, Myeong-Je, Scelonge, Chris, Lenderts, Brian, Chamberlin, Mark, Cushatt, Josh, Wang, Lijuan, Ryan, Larisa, Khan, Tanveer, Chow-Yiu, Julia, Hua, Wei, Yu, Maryanne, Banh, Jenny, Bao, Zhongmeng, Brink, Kent, Igo, Elizabeth, Rudrappa, Bhojaraja, Shamseer, PM, Bruce, Wes, Newman, Lisa, Shen, Bo, Zheng, Peizhong, Bidney, Dennis, Falco, Carl, Register, Jim, Zhao, Zuo-Yu, Xu, Deping, Jones, Todd, Gordon-Kamm, William·2016·The Plant Cell

DOI 10.1105/tpc.16.00124PMC PMC5059793

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Abstract

While transformation of the major monocot crops is currently possible, the process typically remains confined to one or two genotypes per species, often with poor agronomics, and efficiencies that place these methods beyond the reach of most academic laboratories. Here, we report a transformation approach involving overexpression of the maize (Zea mays) Baby boom (Bbm) and maize Wuschel2 (Wus2) genes, which produced high transformation frequencies in numerous previously nontransformable maize inbred lines. For example, the Pioneer inbred PHH5G is recalcitrant to biolistic and Agrobacterium tumefaciens transformation. However, when Bbm and Wus2 were expressed, transgenic calli were recovered from over 40% of the starting explants, with most producing healthy, fertile plants. Another limitation for many monocots is the intensive labor and greenhouse space required to supply immature embryos for transformation. This problem could be alleviated using alternative target tissues that could be supplied consistently with automated preparation. As a major step toward this objective, we transformed Bbm and Wus2 directly into either embryo slices from mature seed or leaf segments from seedlings in a variety of Pioneer inbred lines, routinely recovering healthy, fertile T0 plants. Finally, we demonstrated that the maize Bbm and Wus2 genes stimulate transformation in sorghum (Sorghum bicolor) immature embryos, sugarcane (Saccharum officinarum) callus, and indica rice (Oryza sativa ssp indica) callus.

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Authors: Lowe, K., Wu, E., Wang, N., Hoerster, G., Hastings, C., Cho, M.-J., Scelonge, C., Lenderts, B., Chamberlin, M., Cushatt, J., Wang, L., Ryan, L., Khan, T., Chow-Yiu, J., Hua, W., Yu, M., Banh, J., Bao, Z., Brink, K., Igo, E., Rudrappa, B., Shamseer, P., Bruce, W., Newman, L., Shen, B., Zheng, P., Bidney, D., Falco, C., Register, J., Zhao, Z.-Y., Xu, D., Jones, T., & Gordon-Kamm, W.
Affiliations: Pioneer Hi-Bred (US)
Fruhling Biosciences, West Marredpally, Secunderabad, Telangana 500026, India
DuPont (United States) (US)
BASF Plant Science, Research Triangle Park, North Carolina 27709
Dow AgroSciences, Indianapolis, Indiana 46268
DuPont Pioneer, Johnston, Iowa 50131
ORCID: Wang, Ning
Hoerster, George
Hastings, Craig
Cho, Myeong-Je
Scelonge, Chris
Chamberlin, Mark
Wang, Lijuan
Ryan, Larisa
Yu, Maryanne
Brink, Kent
Bruce, Wes
Newman, Lisa
Register, Jim
Xu, Deping
Jones, Todd
Gordon-Kamm, William
Venue: The Plant Cell
Publisher: Oxford University Press (OUP)
Published: 2016-09
Volume: 28
Issue: 9
Pages: 1998-2015
ISSN: 1040-4651 (ISSN-L)
1532-298X
DOI: 10.1105/tpc.16.00124
PMC: PMC5059793
OpenAlex: W2518225655
Cited by: 881
References: 93
Citations by year: 2026: 93 · 2025: 131 · 2024: 106 · 2023: 101 · 2022: 117 · 2021: 94 · 2020: 91 · 2019: 57 · 2018: 47 · 2017: 38 · 2016: 5
FWCI: 25.46
Type: journal article
Language: en
Open access: hybrid
doi.org
Licence: cc-by
aspb.org/publications/aspb-journals/open-articles (vor, 365 d)
Locations: The Plant Cell (publishedVersion, cc-by)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant tissue culture and regeneration
Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
Keywords: monocot transformation, baby boom, maize, gene overexpression, transformation frequency, transgenic callus, sorghum, sugarcane, Indica rice, inbred lines

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Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation

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