The DOI matches its Crossref record; 93 of the 93 references it cites were found in a registry or an index.
Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation
Open accessAbstract
While transformation of the major monocot crops is currently possible, the process typically remains confined to one or two genotypes per species, often with poor agronomics, and efficiencies that place these methods beyond the reach of most academic laboratories. Here, we report a transformation approach involving overexpression of the maize (Zea mays) Baby boom (Bbm) and maize Wuschel2 (Wus2) genes, which produced high transformation frequencies in numerous previously nontransformable maize inbred lines. For example, the Pioneer inbred PHH5G is recalcitrant to biolistic and Agrobacterium tumefaciens transformation. However, when Bbm and Wus2 were expressed, transgenic calli were recovered from over 40% of the starting explants, with most producing healthy, fertile plants. Another limitation for many monocots is the intensive labor and greenhouse space required to supply immature embryos for transformation. This problem could be alleviated using alternative target tissues that could be supplied consistently with automated preparation. As a major step toward this objective, we transformed Bbm and Wus2 directly into either embryo slices from mature seed or leaf segments from seedlings in a variety of Pioneer inbred lines, routinely recovering healthy, fertile T0 plants. Finally, we demonstrated that the maize Bbm and Wus2 genes stimulate transformation in sorghum (Sorghum bicolor) immature embryos, sugarcane (Saccharum officinarum) callus, and indica rice (Oryza sativa ssp indica) callus.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Overexpression of developmental regulators such as the maize BABY BOOM (BBM) and WUSCHEL 2 (WUS2) genes has increased the transformation efficiency in sorghum, rice, and sugarcane (Lowe et al., 2016;Mookkan et al., 2017).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Lowe et al., 2016
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Livak, K. J., & Schmittgen, T. D. (2001) · Methods · doi:10.1006/meth.2001.1262 · Cited by 180,860
Assaying chimeric genes in plants: The GUS gene fusion system
Jefferson, R. (1987) · Plant Molecular Biology Reporter · doi:10.1007/bf02667740 · Cited by 4,455
Mutation of the mouse klotho gene leads to a syndrome resembling ageing
Kuro-o, M., Matsumura, Y., Aizawa, H., Kawaguchi, H., Suga, T., Utsugi, T., Ohyama, Y., Kurabayashi, M., Kaname, T., Kume, E., et al. (1997) · Nature · doi:10.1038/36285 · Cited by 3,837
Fluorescent proteins from nonbioluminescent Anthozoa species
Matz, M. V., Fradkov, A. F., Labas, Y. A., Savitsky, A. P., Zaraisky, A. G., Markelov, M. L., & Lukyanov, S. (1999) · Nature Biotechnology · doi:10.1038/13657 · Cited by 1,839
Preparation of PCR-Quality Mouse Genomic DNA with Hot Sodium Hydroxide and Tris (HotSHOT)
Truett, G. E., Heeger, P. S., Mynatt, R. L., Truett, A. A., Walker, J. A., & Warman, M. L. (2000) · BioTechniques · doi:10.2144/00291bm09 · Cited by 1,748
Role of WUSCHEL in Regulating Stem Cell Fate in the Arabidopsis Shoot Meristem
Mayer, K., Schoof, H., Haecker, A., Lenhard, M., Jürgens, G., & Laux, T. (1998) · Cell · doi:10.1016/s0092-8674(00)81703-1 · Cited by 1,689
The WUSCHEL gene is required for shoot and floral meristem integrity in Arabidopsis
Laux, T., Mayer, K., Berger, J., & Jürgens, G. (1996) · Development · doi:10.1242/dev.122.1.87 · Cited by 1,270
Plant Microtechnique and Microscopy
Ruzin, S. E. (1999) · Medical Entomology and Zoology · Cited by 1,152
Ectopic Expression of BABY BOOM Triggers a Conversion from Vegetative to Embryonic Growth
Boutilier, K., Offringa, R., Sharma, V. K., Kieft, H., Ouellet, T., Zhang, L., Hattori, J., Liu, C., van Lammeren, A. A. M., Miki, B. L. A., et al. (2002) · The Plant Cell · doi:10.1105/tpc.001941 · Cited by 1,081
Arabidopsis LEAFY COTYLEDON1 Is Sufficient to Induce Embryo Development in Vegetative Cells
Lotan, T., Ohto, M., Yee, K. M., West, M. A. L., Lo, R. S., Kwong, R. W., Yamagishi, K., Fischer, R. L., Goldberg, R. B., & Harada, J. J. (1998) · Cell · doi:10.1016/s0092-8674(00)81463-4 · Cited by 1,063
Research works printed but not linked: 3
- Johansen, D.A. (1940). Plant Microtechnique. (New York: McGraw- Hill).
- Lowe, K., Hoerster, G., Sun, X., Rasco-Gaunt, S., Lazerri, P., Abbitt, S., Glassman, K., and Gordon-Kamm, B. (2002). Maize LEC1 improves transformation in both maize and wheat. In Plant Biotechnology 2002 and Beyond, I.K. Vasil, ed (Dordrecht, The Netherlands: Kluwer Academic Publishers), pp. 255-258.
- Zhao, Z.-Y., Glassman, K., Sewalt, V., Wang, N., Miller, M., Chang, S., Thompson, T., Catron, S., Wu, E., Bidney, D., and Jung, R. (2002). Nutritionally improved transgenic sorghum. In Plant Biotechnology 2002 and Beyond, I.K. Vasil, ed (Dordrecht, The Netherlands: Kluwer Academic Publishers), pp. 413-416.
Cited by
Sorted by citations
CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture
Chen, K., Wang, Y., Zhang, R., Zhang, H., & Gao, C. (2019) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-050718-100049 · Cited by 1,696
Engineering Quantitative Trait Variation for Crop Improvement by Genome Editing
Rodríguez-Leal, D., Lemmon, Z. H., Man, J., Bartlett, M. E., & Lippman, Z. B. (2017) · Cell · doi:10.1016/j.cell.2017.08.030 · Cited by 1,180
Breeding crops to feed 10 billion
Hickey, L. T., Hafeez, A. N., Robinson, H., Jackson, S. A., Leal‐Bertioli, S. C. M., Tester, M. A., Gao, C., Godwin, I. D., Hayes, B. J., & Wulff, B. B. H. (2019) · Nature Biotechnology · doi:10.1038/s41587-019-0152-9 · Cited by 1,098
Genome engineering for crop improvement and future agriculture
Gao, C. (2021) · Cell · doi:10.1016/j.cell.2021.01.005 · Cited by 1,070
Applications of CRISPR–Cas in agriculture and plant biotechnology
Zhu, H., Li, C., & Gao, C. (2020) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-020-00288-9 · Cited by 870
Metadata
- Authors
- Lowe, K., Wu, E., Wang, N., Hoerster, G., Hastings, C., Cho, M.-J., Scelonge, C., Lenderts, B., Chamberlin, M., Cushatt, J., Wang, L., Ryan, L., Khan, T., Chow-Yiu, J., Hua, W., Yu, M., Banh, J., Bao, Z., Brink, K., Igo, E., Rudrappa, B., Shamseer, P., Bruce, W., Newman, L., Shen, B., Zheng, P., Bidney, D., Falco, C., Register, J., Zhao, Z.-Y., Xu, D., Jones, T., & Gordon-Kamm, W.
- Affiliations
- Pioneer Hi-Bred (US)
- Fruhling Biosciences, West Marredpally, Secunderabad, Telangana 500026, India
- DuPont (United States) (US)
- BASF Plant Science, Research Triangle Park, North Carolina 27709
- Dow AgroSciences, Indianapolis, Indiana 46268
- DuPont Pioneer, Johnston, Iowa 50131
- ORCID
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 2016-09
- Volume
- 28
- Issue
- 9
- Pages
- 1998-2015
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- PMC
- PMC5059793
- OpenAlex
- W2518225655
- Cited by
- 881
- References
- 93
- Citations by year
- 2026: 93 · 2025: 131 · 2024: 106 · 2023: 101 · 2022: 117 · 2021: 94 · 2020: 91 · 2019: 57 · 2018: 47 · 2017: 38 · 2016: 5
- FWCI
- 25.46
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- The Plant Cell (publishedVersion, cc-by)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant tissue culture and regeneration
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Keywords
- monocot transformation, baby boom, maize, gene overexpression, transformation frequency, transgenic callus, sorghum, sugarcane, Indica rice, inbred lines
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
