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Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants.
Open accessAbstract
A reproducible system for the generation of fertile, transgenic maize plants has been developed. Cells from embryogenic maize suspension cultures were transformed with the bacterial gene bar using microprojectile bombardment. Transformed calli were selected from the suspension cultures using the herbicide bialaphos. Integration of bar and activity of the enzyme phosphinothricin acetyltransferase (PAT) encoded by bar were confirmed in all bialaphos-resistant callus lines. Fertile transformed maize plants (R0) were regenerated, and of 53 progeny (R1) tested, 29 had PAT activity. All PAT-positive progeny analyzed contained bar. Localized application of herbicide to leaves of bar-transformed R0 and R1 plants resulted in no necrosis, confirming functional activity of PAT in the transgenic plants. Cotransformation experiments were performed using a mixture of two plasmids, one encoding PAT and one containing the nonselected gene encoding [beta]-glucuronidase. R0 plants regenerated from co-transformed callus expressed both genes. These results describe and confirm the development of a system for introduction of DNA into maize.
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Cited by
Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation
Lowe, K., Wu, E., Wang, N., Hoerster, G., Hastings, C., Cho, M.-J., Scelonge, C., Lenderts, B., Chamberlin, M., Cushatt, J., et al. (2016) · The Plant Cell · doi:10.1105/tpc.16.00124 · Cited by 881
Self-citationHigh efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens
Ishida, Y., Saito, H., Ohta, S., Hiei, Y., Komari, T., & Kumashiro, T. (1996) · Nature Biotechnology · doi:10.1038/nbt0696-745 · Cited by 873
Christou, P., Ford, T. L., & Kofron, M. (1991) · Bio/Technology · doi:10.1038/nbt1091-957 · Cited by 561
Generation of Large Numbers of Independently Transformed Fertile Barley Plants
Wan, Y., & Lemaux, P. G. (1994) · Plant Physiology · doi:10.1104/pp.104.1.37 · Cited by 465
Self-citationRapid Production of Multiple Independent Lines of Fertile Transgenic Wheat (Triticum aestivum)
Weeks, J. T., Anderson, O. D., & Blechl, A. E. (1993) · Plant Physiology · doi:10.1104/pp.102.4.1077 · Cited by 423
Metadata
- Authors
- Gordon-Kamm, W. J., Spencer, T. M., Mangano, M. L., Adams, T. R., Daines, R. J., Start, W. G., O'Brien, J. V., Chambers, S. A., Adams, W. R., Willetts, N. G., Rice, T. B., Mackey, C. J., Krueger, R. W., Kausch, A. P., & Lemaux, P. G.
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 1990-07-01
- Volume
- 2
- Issue
- 7
- Pages
- 603-618
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- OpenAlex
- W2163621988
- Cited by
- 765
- References
- 0
- FWCI
- 23.37
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Gordon-Kamm, W. J., Spencer, T. M., Mangano, M. L., Adams, T. R., Daines, R. J., Start, W. G., O'Brien, J. V., Chambers, S. A., Adams, W. R., Willetts, N. G., Rice, T. B., Mackey, C. J., Krueger, R. W., Kausch, A. P., & Lemaux, P. G. (1990). Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants. The Plant Cell. https://doi.org/10.1105/tpc.2.7.603
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