The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
Rapid Production of Multiple Independent Lines of Fertile Transgenic Wheat (Triticum aestivum)
Open accessAbstract
Improvement of wheat (Triticum aestivum) by biotechnological approaches is currently limited by a lack of efficient and reliable transformation methodology. In this report, we detail a protocol for transformation of a highly embryogenic wheat cultivar, Bobwhite. Calli derived from immature embryos, 0.5 to 1 mm long, were bombarded with microprojectiles coated with DNA containing as marker genes the bar gene, encoding phosphinothricin-resistance, and the gene encoding [beta]-glucuronidase (GUS), each under control of a maize ubiquitin promoter. The bombardment was performed 5 d after embryo excision, just after initiation of callus proliferation. The ability of plantlets to root in the presence of 1 or 3 mg/L of bialaphos was the most reliable selection criteria used to identify transformed plants. Stable transformation was confirmed by marker gene expression assays and the presence of the bar sequences in high molecular weight chromosomal DNA of the resultant plants. Nine independent lines of fertile transgenic wheat plants have been obtained thus far, at a frequency of 1 to 2 per 1000 embryos bombarded. On average, 168 d elapsed between embryo excision for bombardment and anthesis of the T0 plants. The transmission of both the resistance phenotype and bar DNA to the T1 generation verified that germline transformation had occurred.
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Cites
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Cited by
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Overexpression of the maize Teosinte Branched1 gene in wheat suppresses tiller development
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Metadata
- Authors
- Weeks, J. T., Anderson, O. D., & Blechl, A. E.
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 1993-08-01
- Volume
- 102
- Issue
- 4
- Pages
- 1077-1084
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- OpenAlex
- W2170434347
- Cited by
- 423
- References
- 11
- FWCI
- 12.12
- Type
- journal article
- Language
- en
- Open access
- green
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- Weeks, J. T., Anderson, O. D., & Blechl, A. E. (1993). Rapid Production of Multiple Independent Lines of Fertile Transgenic Wheat (Triticum aestivum). Plant Physiology. https://doi.org/10.1104/pp.102.4.1077
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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