Simultaneous measurement of intracellular calcium and electrical activity from patterned neural networks in culture

Jimbo, Y.; Robinson, H.P.C.; Kawana, A.

doi:10.1109/10.238465

Cited by 192Neuroscience and Neural EngineeringNeural dynamics and brain functionNeuroscience and Neuropharmacology Research

Simultaneous measurement of intracellular calcium and electrical activity from patterned neural networks in culture

Jimbo, Y., Robinson, H.P.C., Kawana, A.·1993·IEEE Transactions on Biomedical Engineering

DOI 10.1109/10.238465

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Abstract

Multisite extracellular electrical activity and intracellular calcium were recorded simultaneously. Electrical signals were measured using microelectrode array substrates. A novel cell positioning technique was combined with a method for controlling neurite outgrowth, which allowed cell-electrode contacts to be established easily, thus facilitating the electrical recording. Intracellular calcium was measured optically using the indicator fluo 3. Under low-magnesium conditions, cultured rat cortical neurons showed periodic transients of fluo-3 fluorescence, which were synchronized with the periodic bursting observed electrically. The intervals between bursts could be determined by electrical stimulation through the substrate electrodes. The results suggest that functional synaptic connections are formed in the culture system.

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With surface topologies on the substrate, axonal outgrowth of embryonic rat nerve cells was observed and electrical activity was recorded in a microstructure with 16 electrodes connected with grooves.
“Having surface topologies on the substrate, axonal outgrowth of embryonic nerve cells from rats was observed and electrical activity was recorded in a microstructure with 16 electrodes that were connected with grooves (Jimbo and Kawana, 1992; Jimbo et al., 1993)”
From A critical review of interfaces with the peripheral nervous system for the control of neuroprostheses and hybrid bionic systems (2005) · cites this paper as Jimbo et al., 1993

Electrical stimulation and recording from cultured neurons using a planar electrode array
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Self-citation
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BackgroundInfluential
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Authors: Jimbo, Y., Robinson, H. P. C., & Kawana, A.
Affiliations: NTT (Japan) (JP)
ORCID: Jimbo, Y.
Robinson, H.P.C.
Venue: IEEE Transactions on Biomedical Engineering
Publisher: Institute of Electrical and Electronics Engineers (IEEE)
Published: 1993
Volume: 40
Issue: 8
Pages: 804-810
ISSN: 0018-9294 (ISSN-L)
DOI: 10.1109/10.238465
OpenAlex: W2051002031
Cited by: 192
References: 0
Citations by year: 2025: 1 · 2024: 1 · 2023: 2 · 2021: 3 · 2020: 2 · 2019: 3 · 2018: 2 · 2017: 1 · 2016: 7 · 2015: 1 · 2014: 5 · 2013: 8 · 2012: 3
FWCI: 1.43
Type: journal article
Language: en
Open access: closed
Licence: ieeexplore.ieee.org/Xplorehelp/downloads/license-information/IEEE.html (vor)
Locations: IEEE Transactions on Biomedical Engineering (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neural Engineering
Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords: intracellular calcium, microelectrode arrays, fluo-3, cortical neurons, neuronal bursting, electrical stimulation, cell positioning, synaptic connectivity, simultaneous recording
MeSH terms: Animals, Calcium, Cells, Cultured, Cerebral Cortex, Cytological Techniques, Electric Stimulation, Electrophysiology, Embryo, Mammalian, Equipment Design, Magnesium, Microelectrodes, Nerve Net, Neurons, Semiconductors, Rats

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Simultaneous measurement of intracellular calcium and electrical activity from patterned neural networks in culture

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