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Simultaneous Imaging of Different Focal Planes in Fluorescence Microscopy for the Study of Cellular Dynamics in Three Dimensions
Open accessAbstract
The imaging of cellular dynamics in three dimensions using a standard microscope is severely limited due to the fact that only one focal plane can be imaged at a given point in time. Here we present a modification of the classical microscope design with which two or more focal planes can be imaged simultaneously. This is achieved by a modification of the emission pathway of a standard microscope. The efficacy of the design is shown by imaging bead samples and an FcRn-green fluorescent protein expressing tubule that leaves a sorting endosome and subsequently exocytoses at the plasma membrane.
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Cites
Inoué, S., & Spring, K. R. (1997) · doi:10.1007/978-1-4615-5859-0 · Cited by 326
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Single Cell Optical Imaging and Spectroscopy
Stender, A. S., Marchuk, K., Liu, C., Sander, S., Meyer, M. W., Smith, E. A., Neupane, B., Wang, G., Li, J., Cheng, J.-X., et al. (2013) · Chemical Reviews · doi:10.1021/cr300336e · Cited by 292
Prabhat, P., Gan, Z., Chao, J., Ram, S., Vaccaro, C., Gibbons, S., Ober, R. J., & Ward, E. S. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0700337104 · Cited by 157
Self-citationSingle-molecule spectroscopy and imaging over the decades
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Metadata
- Authors
- Prabhat, P., Ram, S., Ward, E. S., & Ober, R. J.
- Venue
- IEEE Transactions on Nanobioscience
- Published
- 2004-12
- Volume
- 3
- Issue
- 4
- Pages
- 237-242
- ISSN
- 1536-1241 (ISSN-L)
- OpenAlex
- W2128323873
- Cited by
- 188
- References
- 1
- FWCI
- 2.02
- Type
- journal article
- Language
- en
- Open access
- green
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- Prabhat, P., Ram, S., Ward, E. S., & Ober, R. J. (2004). Simultaneous Imaging of Different Focal Planes in Fluorescence Microscopy for the Study of Cellular Dynamics in Three Dimensions. IEEE Transactions on Nanobioscience. https://doi.org/10.1109/tnb.2004.837899
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