The association between vaginal bacterial composition and miscarriage: a nested case-control study
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OBJECTIVE: To characterise vaginal bacterial composition in early pregnancy and investigate its relationship with first and second trimester miscarriages. DESIGN: Nested case-control study. SETTING: Queen Charlotte's and Chelsea Hospital, Imperial College Healthcare NHS Trust, London. POPULATION: 161 pregnancies: 64 resulting in first trimester miscarriage, 14 in second trimester miscarriage and 83 term pregnancies. METHODS: Prospective profiling and comparison of vaginal bacteria composition using 16S rRNA gene-based metataxonomics from 5 weeks' gestation in pregnancies ending in miscarriage or uncomplicated term deliveries matched for age, gestation and body mass index. MAIN OUTCOME MEASURES: Relative vaginal bacteria abundance, diversity and richness. Pregnancy outcomes defined as first or second trimester miscarriage, or uncomplicated term delivery. RESULTS: First trimester miscarriage associated with reduced prevalence of Lactobacillus spp.-dominated vaginal microbiota classified using hierarchical clustering analysis (65.6 versus 87.7%; P = 0.005), higher alpha diversity (mean Inverse Simpson Index 2.5 [95% confidence interval 1.8-3.0] versus 1.5 [1.3-1.7], P = 0.003) and higher richness 25.1 (18.5-31.7) versus 16.7 (13.4-20), P = 0.017), compared with viable pregnancies. This was independent of vaginal bleeding and observable before first trimester miscarriage diagnosis (P = 0.015). Incomplete/complete miscarriage associated with higher proportions of Lactobacillus spp.-depleted communities compared with missed miscarriage. Early pregnancy vaginal bacterial stability was similar between miscarriage and term pregnancies. CONCLUSIONS: These findings associate the bacterial component of vaginal microbiota with first trimester miscarriage and indicate suboptimal community composition is established in early pregnancy. While further studies are required to elucidate the mechanism, vaginal bacterial composition may represent a modifiable risk factor for first trimester miscarriage. TWEETABLE ABSTRACT: Vaginal bacterial composition in first trimester miscarriage is associated with reduced Lactobacillus spp. abundance and is independent of vaginal bleeding.
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High stress is associated with miscarriage risk (aOR 1.46; 95% CI 1.16-1.77).
“Our review of the evidence also found that high stress is associated with miscarriage risk (aOR 1·46; 95% CI 1·161·77; Table 6) 66,71,73-77”
From Miscarriage matters: the epidemiological, physical, psychological, and economic costs of early pregnancy loss (2021) · cites this paper as [74]
The Results report associations between vaginal bacterial composition and miscarriage, not stress. Searches of the indexed paper for high stress, psychological stress, and the stated odds ratio found no passage supporting the stress association or its aOR and confidence interval. (LLM, conf=0.75)
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- Authors
- Al‐Memar, M., Bobdiwala, S., Fourie, H., Mannino, R., Lee, Y., Smith, A., Marchesi, Jr., Timmerman, D., Bourne, T., Bennett, P., & MacIntyre, D.
- Affiliations
- Imperial College London (GB)
- Imperial College Healthcare NHS Trust (GB)
- Cardiff University (GB)
- Universitair Ziekenhuis Leuven (BE)
- Tommy’s National Centre for Miscarriage Research Imperial College London London UK
- Venue
- BJOG: An International Journal of Obstetrics & Gynaecology
- Publisher
- Wiley
- Published
- 2019-10-31
- Volume
- 127
- Issue
- 2
- Pages
- 264-274
- ISSN
- 0306-5456 (ISSN-L)
- 1470-0328
- 1471-0528
- PMC
- PMC6972675
- OpenAlex
- W2978305519
- Cited by
- 140
- References
- 60
- Citations by year
- 2026: 13 · 2025: 16 · 2024: 22 · 2023: 29 · 2022: 29 · 2021: 27 · 2020: 4
- FWCI
- 11.41
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- BJOG An International Journal of Obstetrics & Gynaecology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- ORCA Online Research @Cardiff (Cardiff University) (acceptedVersion)
- Lirias (submittedVersion, cc-by)
- UWE Research Repository (UWE Bristol) (submittedVersion, cc-by)
- Spiral (Imperial College London) (submittedVersion, other-oa)
- Funders
- March of Dimes Foundation
- NIHR Biomedical Research Centre, Royal Marsden NHS Foundation Trust/Institute of Cancer Research
- National Institute for Health and Care Research (P45272)
- Imperial College London (P45272)
- Imperial College Healthcare NHS Trust (P45272)
- Tommy's
- Tommy's National Centre for Miscarriage Research (P62774)
- Medical Research Council (MR/L009226/1)
- NIHR Imperial Biomedical Research Centre
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Epidemiology › Preterm Birth and Chorioamnionitis
- Keywords
- vaginal microbiota, miscarriage, first-trimester miscarriage, second-trimester miscarriage, Lactobacillus, bacterial diversity, bacterial richness, early pregnancy, missed abortion, vaginal bleeding, metataxonomics, 16S rRNA gene sequencing
- MeSH terms
- Abortion, Spontaneous, Adult, Female, Humans, London, Pregnancy, Pregnancy Trimester, First, Pregnancy Trimester, Second, Prospective Studies, RNA, Ribosomal, 16S, Vagina, Case-Control Studies, Microbiota
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