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Cited by 4Viral Infectious Diseases and Gene Expression in InsectsBlood transfusion and managementAnimal testing and alternatives

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Up‐to‐Date Research on the Composition of Blood Slaughterhouse By‐Products Derived FBS Substitutes and Their Applicability to Animal Cell Culture

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Lee, Da Young, Choi, Yeongwoo, Han, Dahee, Park, Jinmo, Kim, Jin Soo, Mariano, Ermie Jr., Park, Ji Won, Namkung, Seok, Lee, Seung Yun, Choi, Inho, Joo, Seon‐Tea, Hur, Sun Jin·2025·Journal of Food Science
DOI 10.1111/1750-3841.70492PMC PMC12355341
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Abstract

Fetal bovine serum (FBS) is a critical media component in cell culture, but it is also expensive and highly controversial from an animal welfare level, being of fetal blood origin. Therefore, our study aimed to develop FBS substitutes from slaughterhouse waste blood. We pooled the blood from slaughterhouse by‐products (BSBPs) of three major livestock species (cattle, pigs, and chickens) and performed nutritional and hematological analyses of serum, plasma, whole blood, and cruor. Our substitutes were cultured with C2C12 cells, and the serum of each livestock species showed a cell proliferation effect. Nonetheless, the nutritional and hematological profiles of BSBPs from this study can provide basic component information for using livestock blood as a raw material for various preparations. Furthermore, the cell culture results using C2C12 cells indicate that using BSBPs can solve expensive cell culture costs and animal ethics issues by replacing FBS.

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Metadata

Authors
Lee, D. Y., Choi, Y., Han, D., Park, J., Kim, J. S., Mariano, E. J., Park, J. W., Namkung, S., Lee, S. Y., Choi, I., Joo, S., & Hur, S. J.
Affiliations
  • Chung-Ang University (KR)
  • Gyeongsang National University (KR)
  • Yeungnam University (KR)
Corresponding author
Hur, Sun Jin
ORCID
Venue
Journal of Food Science
Publisher
Wiley
Published
2025-08
Volume
90
Issue
8
Pages
e70492-e70492
ISSN
  • 0022-1147 (ISSN-L)
  • 1750-3841
DOI
10.1111/1750-3841.70492
PMC
PMC12355341
OpenAlex
W4413139285
Cited by
4
References
40
Citations by year
2026: 4
FWCI
1.20
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Food Science (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
Funders
  • National Research Foundation of Korea
  • Chung-Ang University
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
  • Health Sciences › Medicine › Hematology › Blood transfusion and management
  • Health Sciences › Veterinary › Small Animals › Animal testing and alternatives
Keywords
slaughterhouse by-products, blood waste, C2C12 myoblasts, cell proliferation, hematological analysis, nutritional composition, cattle blood, pig blood, chicken blood, serum, plasma, animal welfare
MeSH terms
Abattoirs, Animals, Cattle, Cell Line, Chickens, Culture Media, Swine, Waste Products, Cell Culture Techniques, Serum, Cell Proliferation, Mice, Livestock

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