The potential of bacteria isolated from ruminal contents of seaweed‐eating N orth R onaldsay sheep to hydrolyse seaweed components and produce methane by anaerobic digestion in vitro
Open accessAbstract
Summary: The production of methane biofuel from seaweeds is limited by the hydrolysis of polysaccharides. The rumen microbiota of seaweed‐eating N orth R onaldsay sheep was studied for polysaccharidic bacterial isolates degrading brown‐seaweed polysaccharides. Only nine isolates out of 65 utilized > 90% of the polysaccharide they were isolated on. The nine isolates (eight P revotella spp. and one C lostridium butyricum ) utilized whole L aminaria hyperborea extract and a range of seaweed polysaccharides, including alginate (seven out of nine isolates), laminarin and carboxymethylcellulose (eight out of nine isolates); while two out of nine isolates additionally hydrolysed fucoidan to some extent. Crude enzyme extracts from three of the isolates studied further had diverse glycosidases and polysaccharidase activities; particularly against laminarin and alginate (two isolates were shown to have alginate lyase activity) and notably fucoidan and carageenan (one isolate). In serial culture rumen microbiota hydrolysed a range of seaweed polysaccharides (fucoidan to a notably lesser degree) and homogenates of L . hyperborea , mixed F ucus spp. and A scophyllum nodosum to produce methane and acetate. The rumen microbiota and isolates represent potential adjunct organisms or enzymes which may improve hydrolysis of seaweed components and thus improve the efficiency of seaweed anaerobic digestion for methane biofuel production.
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Rumen microbiota of macroalgae-consuming North Ronaldsay sheep adapted to efficiently digest this vegetation.
“Similarly, rumen microbiota of macroalgaconsuming North Ronaldsay sheep adapted to efficiently digest this vegetation (Williams et al., 2012).”
From Novel Lethal Clostridial Infection in Florida Manatees (Trichechus manatus latirostris): Cause of the 2013 Unusual Mortality Event in the Indian River Lagoon (2022) · cites this paper as Williams et al., 2012
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Stewart, C. S., Flint, H. J., & Bryant, M. P. (1997) · The Rumen Microbial Ecosystem · doi:10.1007/978-94-009-1453-7_2 · Cited by 772 · see citing works
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Alaswad, A., Dassisti, M., Prescott, T., & Olabi, A. G. (2015) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2015.07.058 · Cited by 281 · see citing works
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“consuming North Ronaldsay sheep adapted to efficiently digest this vegetation (Williams et al., 2012).”
Background
- Authors
- Williams, A. G., Withers, S., & Sutherland, A. D.
- Affiliations
- Glasgow Caledonian University (GB)
- Corresponding author
- Sutherland, Alastair D.
- Venue
- Microbial Biotechnology
- Publisher
- Wiley
- Published
- 2012-11-22
- Volume
- 6
- Issue
- 1
- Pages
- 45-52
- ISSN
- 1751-7915 (ISSN-L)
- PMC
- PMC3815384
- OpenAlex
- W1520061278
- Cited by
- 41
- References
- 1
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 3 · 2023: 7 · 2022: 3 · 2021: 1 · 2020: 3 · 2019: 5 · 2018: 2 · 2016: 4 · 2015: 3 · 2014: 2 · 2013: 1
- FWCI
- 3.62
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Microbial Biotechnology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Keywords
- rumen microbiota, Prevotella, Clostridium butyricum, alginate, laminarin, fucoidan, carageenan, alginate lyase, glycoside hydrolases, polysaccharidases, Laminaria hyperborea, Fucus, Ascophyllum nodosum, seaweed biorefinery
- MeSH terms
- Acetates, Anaerobiosis, Animals, Bacteria, Anaerobic, Digestion, Feeding Behavior, Hydrolysis, Methane, Polysaccharides, Rumen, Seaweed, Sheep, Prevotella, Clostridium butyricum
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