Determination of seminal oxidation-reduction potential (ORP) as an easy and cost-effective clinical marker of male infertility
Open accessAbstract
Oxidative stress (OS) is an important contributing factor to male infertility. While previous methods to measure seminal OS are time-consuming and limited to the use of freshly produced semen, oxidation reduction potential (ORP) is easier and quicker to perform and can also be used in frozen semen. Therefore, this study evaluated the clinical utility of ORP as a potential marker of male infertility. ORP was measured in semen samples from 293 patients and 15 fertile controls and categorised according to WHO criteria as normozoospermic, oligozoospermic, asthenozoospermic, teratozoospermic and oligoasthenoteratozoospermic. Receiver operating characteristic (ROC) curves were generated to differentiate these categories. Semen parameters were significantly different when subjects were grouped as control and patients or between the patient and normozoospermic group for concentration and morphology. ORP levels were significantly different between the control and normozoospermic group. When subjects were grouped based on concentration, motility, morphology or a combination of these, the area under the ROC curve, sensitivity, specificity, positive predictive value and cut-off values were significantly different. These differences were significant when combined with ORP and grouped with any two sperm abnormalities. In conclusion, ORP is a quick, easy, cost-effective and reliable marker of semen quality as well as oxidative stress for use in a clinical setting.
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Recent progress has been made in terms of identification of relevant patients for improving diagnostic capabilities of antioxidant capacity or redox balance by means of ORP.
“For the latter, recent progress has been made in terms of the identification of relevant patients [60,66,67].”
From The Sixth Edition of the WHO Manual for Human Semen Analysis: A Critical Review and SWOT Analysis (2021) · cites this paper as [66]
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Relationship amongst teratozoospermia, seminal oxidative stress and male infertility
Agarwal, A., Tvrda, E., & Sharma, R. (2014) · Reproductive Biology and Endocrinology · doi:10.1186/1477-7827-12-45 · Cited by 176 · see citing works
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- Agarwal, A., Ahmad, G., & Sharma, R. (2015). Reference values of reactive oxygen species in seminal ejaculates using chemiluminescence assay. Journal of Assisted Reproduction and Genetics, 32, 1721-1729.
- Agarwal, A., Gupta, S., & Sharma, R. (2016). Oxidation-Reduction potential measurement in ejaculated semen samples. In Agarwal A., Gupta S., Sharma R. (eds) Andrological evaluation of male infertility: A laboratory guide, Vol. 22 (pp. 165-170). Switzerland: Springer Nature, Springer International Publishing, AG.
- Agarwal, A., & Wang, S. M. (2017). Clinical relevance of oxidation-reduction potential in the evaluation of male infertility. Urology, 2017(104), 84-89.
- Agarwal, A., Zini, A., & Sigman, M. (2013). Is sperm DNA integrity assess- ment useful? Journal of Urology, 190, 1645-1647.
- Cavallini, G. (2006). Male idiopathic oligoasthenoteratozoospermia. Asian Journal of Andrology, 8, 143-157.
- Esteves, S. C. (2010). Clinical relevance of routine semen analysis and con- troversies surrounding the 2010 World Health Organization criteria for semen examination. International Brazilian Journal of Urology, 40, 443-453.
- Esteves, S. C., & Agarwal, A. (2011). Novel concepts in male infertility. International Brazilian Journal of Urology, 37, 5-15.
- Guzick, D. S., Overstreet, J. W., Factor-Litvak, P., Brazil, C. K., Nakajima, S. T., Coutifaris, C., … Network National Cooperative Reproductive Medicine. (2001). Sperm morphology, motility, and concentration in fertile and infertile men. New England Journal of Medicine, 345, 1388-1393.
- Roychoudhury, S., Sharma, R., Sikka, S., & Agarwal, A. (2016). Diagnostic application of total antioxidant capacity in seminal plasma to assess ox- idative stress in male factor infertility. Journal of Assisted Reproduction and Genetics, 33, 627-635.
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The Sixth Edition of the WHO Manual for Human Semen Analysis: A Critical Review and SWOT Analysis
Boitrelle, F., Shah, R., Saleh, R., Henkel, R., Kandil, H., Chung, E., Vogiatzi, P., Zini, A., Arafa, M., & Agarwal, A. (2021) · Life · doi:10.3390/life11121368 · Cited by 271 · see citing works
“For the latter, recent progress has been made in terms of the identification of relevant patients [60,66,67].”
BackgroundSelf-citationGarcia-Segura, S., Ribas-Maynou, J., Lara-Cerrillo, S., Garcia-Peiró, A., Castel, A. B., Benet, J., & Oliver-Bonet, M. (2020) · Biology · doi:10.3390/biology9090262 · Cited by 21 · see citing works
- Authors
- Agarwal, A., Henkel, R., Sharma, R., Tadros, N. N., & Sabanegh, E.
- Affiliations
- Cleveland Clinic (US)
- University of the Western Cape (ZA)
- SIU Medicine (US)
- Corresponding author
- Agarwal, A.
- Venue
- Andrologia
- Publisher
- Wiley
- Published
- 2017-10-23
- Volume
- 50
- Issue
- 3
- Pages
- e12914
- ISSN
- 0303-4569 (ISSN-L)
- OpenAlex
- W2766537744
- Cited by
- 44
- References
- 1
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 7 · 2023: 4 · 2022: 5 · 2021: 2 · 2020: 14 · 2019: 3 · 2018: 3 · 2017: 1
- FWCI
- 6.67
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Andrologia (publishedVersion)
- PubMed (publishedVersion)
- UWC Research Repository (University of the Western Cape) (submittedVersion)
- Funders
- American Center for Reproductive Medicine
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
- Health Sciences › Medicine › Reproductive Medicine › Reproductive Health and Technologies
- Health Sciences › Health Professions › General Health Professions › Male Reproductive Health Studies
- Keywords
- redox potential, seminal plasma, male infertility, oxidative stress, semen quality, sperm abnormalities, oligozoospermia, asthenozoospermia, teratozoospermia, frozen semen, clinical biomarkers
- MeSH terms
- Adult, Humans, Infertility, Male, Male, Oxidation-Reduction, Semen, Sperm Count, Sperm Motility, Spermatozoa, Oxidative Stress, Semen Analysis
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