Identification lncRNA LOC102551149/miR‐23a‐5p pathway in hepatic fibrosis
Abstract
Background Hepatic fibrosis is a worldwide incurable disease; due to the complex and unclear mechanism, there lack the effective therapeutic targets. However, the mechanism of miR-23a-5p underling this pathological process is largely not clear. The purpose of this study was to investigate the role of miR-23a-5p in hepatic fibrosis and HSC activation. Methods The content of miR-23a-5p in hepatic fibrosis induced by N-nitrosodimethylamine (NDMA) and HSC activation induced by platelet-derived growth factor (PDGF) was detected by qRT-PCR. H&E staining, Masson staining and Shear wave electrography (SWE) were used to detect the degree of hepatic fibrosis. Immunohistochemistry staining, qRT-PCR and Western blot detect the related markers of liver fibrosis or HSC activation, as well as the related pathway genes and proteins. Dual-luciferase reporter system verifies the interaction between miR-23a-5p with PTEN or miR-23a-5p with lncRNA LOC102551149 in HSC-T6. siRNA and miRNA mimic transfer to HSC-T6 to detect the function of lncRNA LOC102551149 and miR-23a-5p on HSC activation. Results After hepatic fibrosis and HSC activation happened, the expression of miR-23a-5p was up-regulated, whereas anti-miR-23a-5p can alleviate hepatic fibrosis and HSC activation. Further research shows miR-23a-5p can target PTEN and degrade it, causing activation of PI3K/Akt/mTOR/Snail pathway. lncRNA LOC102551149 can be used as a competition endogenous RNA (ceRNA) targeting miR-23a-5p through base pairing,…
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Following liver fibrosis and hepatic stellate cell activation, up-regulated miR-23a-5p targets PTEN for degradation, activating the PI3K/Akt/mTOR/Snail pathway.
“Following the occurrence of liver fibrosis and the activation of hepatic stellate cells, the up-regulated expression of miR-23a-5p targets phosphatase and tensin homolog (PTEN) for degradation, thereby activating the PI3K/Akt/mTOR/Snail pathway, providing a new target for improving fibrosis [18].”
From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [18]
Cites
Origins and Mechanisms of miRNAs and siRNAs
Carthew, R. W., & Sontheimer, E. J. (2009) · Cell · doi:10.1016/j.cell.2009.01.035 · Cited by 5,023 · see citing works
Bataller, R., & Brenner, D. A. (2005) · Journal of Clinical Investigation · doi:10.1172/jci24282 · Cited by 4,958 · see citing works
Hepatic Stellate Cells: Protean, Multifunctional, and Enigmatic Cells of the Liver
Friedman, S. L. (2008) · Physiological Reviews · doi:10.1152/physrev.00013.2007 · Cited by 2,842 · see citing works
Mechanisms of Hepatic Fibrogenesis
Friedman, S. L. (2008) · Gastroenterology · doi:10.1053/j.gastro.2008.03.003 · Cited by 2,640 · see citing works
Schuppan, D., & Afdhal, N. H. (2008) · The Lancet · doi:10.1016/s0140-6736(08)60383-9 · Cited by 2,089 · see citing works
Hepatic stellate cells as key target in liver fibrosis
Higashi, T., Friedman, S. L., & Hoshida, Y. (2017) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2017.05.007 · Cited by 1,485 · see citing works
Bataller, R. A. (2005) · Journal of Clinical Investigation · doi:10.1172/jci200524282c1 · Cited by 1,480 · see citing works
Liver inflammation and fibrosis
Koyama, Y., & Brenner, D. A. (2017) · Journal of Clinical Investigation · doi:10.1172/jci88881 · Cited by 1,295 · see citing works
Yang, H., Kong, W. W., He, L., Zhao, J., O’Donnell, J. D., Wang, J., Wenham, R. M., Coppola, D., Kruk, P. A., Nicosia, S. V., et al. (2008) · Cancer Research · doi:10.1158/0008-5472.can-07-2488 · Cited by 1,051 · see citing works
MicroRNAs: key players in the immune system, differentiation, tumorigenesis and cell death
Schickel, R., Boyerinas, B. M., Sm, P., & Peter, M. E. (2008) · Oncogene · doi:10.1038/onc.2008.274 · Cited by 713 · see citing works
Huse, J. T., Brennan, C., Hambardzumyan, D., Wee, B., Pena, J., Rouhanifard, S. H., Sohn-Lee, C., le Sage, C., Agami, R., Tuschl, T., et al. (2009) · Genes & Development · doi:10.1101/gad.1777409 · Cited by 511 · see citing works
miR-23a functions downstream of NFATc3 to regulate cardiac hypertrophy
Lin, Z., Murtaza, I., Wang, K., Jiao, J., Gao, J., & Li, P. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0811371106 · Cited by 360 · see citing works
Molecular mechanisms of hepatic fibrogenesis
Parsons, C. J., Takashima, M., & Rippe, R. (2007) · Journal of Gastroenterology and Hepatology · doi:10.1111/j.1440-1746.2006.04659.x · Cited by 323 · see citing works
Sayed, D. H., He, M., Hong, C., Gao, S., Rane, S., Yang, Z., & Abdellatif, M. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.109207 · Cited by 313 · see citing works
The liver-enriched lnc-LFAR1 promotes liver fibrosis by activating TGFβ and Notch pathways
Zhang, K., Han, X., Zhang, Z., Zheng, L., Hu, Z., Yao, Q., Cui, H., Shu, G., Si, M., Li, C., et al. (2017) · Nature Communications · doi:10.1038/s41467-017-00204-4 · Cited by 243 · see citing works
Competing endogenous RNA networks and gastric cancer
Guo, L.-L. (2015) · World Journal of Gastroenterology · doi:10.3748/wjg.v21.i41.11680 · Cited by 189 · see citing works
Wang, J., Chu, E. S., Chen, H., Man, K., Go, M. Y. Y., Huang, X. R., Lan, H. Y., Sung, J. J. �., & Yu, J. Q. (2014) · Oncotarget · doi:10.18632/oncotarget.2621 · Cited by 187 · see citing works
Böttcher, K., & Pinzani, M. (2017) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2017.05.016 · Cited by 183 · see citing works
Cao, M., Seike, M., Soeno, C., Mizutani, H., Kitamura, K., Minegishi, Y., Noro, R., Yoshimura, A., Cai, L., & Gemma, A. (2012) · International Journal of Oncology · doi:10.3892/ijo.2012.1535 · Cited by 168 · see citing works
Alvarez, M. L., Khosroheidari, M., Eddy, E., & Kiefer, J. (2013) · PLoS ONE · doi:10.1371/journal.pone.0077468 · Cited by 162 · see citing works
Yu, F., Chen, B., Dong, P., & Zheng, J. (2017) · Molecular Therapy · doi:10.1016/j.ymthe.2016.10.015 · Cited by 132 · see citing works
Yu, F., Lu, Z., Cai, J., Huang, K., Chen, B., Li, G., Dong, P., & Zheng, J. (2015) · Cell Cycle · doi:10.1080/15384101.2015.1120917 · Cited by 126 · see citing works
George, J., & Tsutsumi, M. (2007) · Gene Therapy · doi:10.1038/sj.gt.3302929 · Cited by 125 · see citing works
MicroRNA ‐101 suppresses liver fibrosis by targeting the TGF β signalling pathway
Tu, X., Zhang, H., Zhang, J., Zhao, S., Zheng, X., Zhang, Z., Zhu, J., Chen, J., Dong, L., Zang, Y., et al. (2014) · The Journal of Pathology · doi:10.1002/path.4373 · Cited by 125 · see citing works
Leti, F., Legendre, C., Still, C. D., Chu, X., Petrick, A. T., Gerhard, G. S., & DiStefano, J. K. (2017) · Translational research · doi:10.1016/j.trsl.2017.09.001 · Cited by 125 · see citing works
Zhu, L., Liu, T., Tang, H., Tian, R., Su, C., Liu, M., & Li, X. (2010) · FEBS Journal · doi:10.1111/j.1742-4658.2010.07773.x · Cited by 86 · see citing works
MicroRNA-21 accelerates hepatocyte proliferation in vitro via PI3K/Akt signaling by targeting PTEN
Bai, Y.-N., Yu, Z., Luo, L.-X., jiang, Y., Xia, Q., & Yong, Z. (2013) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2013.12.047 · Cited by 78 · see citing works
Lin, H., Huang, Z., Liu, J., Qiu, Y., Tao, Y., Wang, M., Yao, H., Hou, K., Gu, F., & Xu, X. (2018) · Scientific Reports · doi:10.1038/s41598-018-28519-2 · Cited by 73 · see citing works
Regulation of Hepatic Stellate Cells and Fibrogenesis by Fibroblast Growth Factors
Schumacher, J. D., & Guo, G. L. (2016) · BioMed Research International · doi:10.1155/2016/8323747 · Cited by 70 · see citing works
Roy, S., Benz, F., Cardenas, D. V., Vucur, M., Gautheron, J., Schneider, A. T., Hellerbrand, C., Pottier, N., Alder, J., Tacke, F., et al. (2015) · Journal of Digestive Diseases · doi:10.1111/1751-2980.12266 · Cited by 62 · see citing works
Identification of a Novel lincRNA-p21-miR-181b-PTEN Signaling Cascade in Liver Fibrosis
Yu, F., Lu, Z., Chen, B., Dong, P., & Zheng, J. (2016) · Mediators of Inflammation · doi:10.1155/2016/9856538 · Cited by 61 · see citing works
MicroRNAs and ceRNAs: therapeutic implications of RNA networks
Giza, D. E., Vasilescu, C., & Călin, G. A. (2014) · Expert Opinion on Biological Therapy · doi:10.1517/14712598.2014.920812 · Cited by 60 · see citing works
High-throughput validation of ceRNA regulatory networks
Chiu, H., Martínez, M. R., Bansal, M., Subramanian, A., Golub, T. R., Yang, X., Sumazin, P., & Califano, A. (2017) · BMC Genomics · doi:10.1186/s12864-017-3790-7 · Cited by 58 · see citing works
Okada, H., Honda, M., Campbell, J. S., Takegoshi, K., Sakai, Y., Yamashita, T., Shirasaki, T., Takabatake, R., Nakamura, M., Tanaka, T., et al. (2015) · Cancer Science · doi:10.1111/cas.12730 · Cited by 47 · see citing works
Hao, X.-J., Xu, C., Wang, J., Li, X., Wang, M.-M., Gu, Y.-H., & Liang, Z. (2018) · Journal of Receptors and Signal Transduction · doi:10.1080/10799893.2019.1585452 · Cited by 38 · see citing works
Zhang, Q.-Q., Xu, M., Qu, Y., Hu, J., Li, Z., Zhang, Q., & Lu, L. (2014) · Europe PMC (PubMed Central) · Cited by 35 · see citing works
Phosphatidylinositol 3-kinase/Akt pathway regulates hepatic stellate cell apoptosis
Wang, Y., Jiang, X., Liu, L., & Jiang, H. (2008) · World Journal of Gastroenterology · doi:10.3748/wjg.14.5186 · Cited by 33 · see citing works
Correlation between miR-23a and onset of hepatocellular carcinoma
Bao, L., Zhao, J., Dai, X., Wang, Y., Ma, R., Su, Y., Cui, H., Niu, J., Bai, S., Xiao, Z., et al. (2014) · Clinics and Research in Hepatology and Gastroenterology · doi:10.1016/j.clinre.2013.12.002 · Cited by 32 · see citing works
Self-citationDuan, X., Liu, X., Li, Y., Cao, Y., Silayiding, A., Zhang, R., & Wang, J. (2019) · The Kaohsiung Journal of Medical Sciences · doi:10.1002/kjm2.12108 · Cited by 32 · see citing works
Ge, S., Xie, J., Liu, F., He, J., & He, J. (2015) · Journal of Cellular Biochemistry · doi:10.1002/jcb.25116 · Cited by 30 · see citing works
MicroRNA-96 Promotes Schistosomiasis Hepatic Fibrosis in Mice by Suppressing Smad7
Luo, X., Zhang, D., Xie, J., Su, Q., He, X., Bai, R., Gao, G., & Pan, W. (2018) · Molecular Therapy — Methods & Clinical Development · doi:10.1016/j.omtm.2018.10.002 · Cited by 22 · see citing works
Li, Z., Zhao, L., Han, Q., & Zhu, X. H. (2016) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2016.07.059 · Cited by 6 · see citing works
Flagged first, then by citations
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Wang, C., Zhou, H., Wu, R. T., Guo, Y., Gong, L., Fu, K., Ma, C., Peng, C. Y., & Li, Y. (2022) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2022.114040 · Cited by 31 · see citing works
The miR-23–27–24 cluster: an emerging target in NAFLD pathogenesis
Ru, L., Wang, X.-M., & Niu, J.-Q. (2022) · Acta Pharmacologica Sinica · doi:10.1038/s41401-021-00819-w · Cited by 21 · see citing works
Role of noncoding RNAs in liver fibrosis
Li, Q., Gong, T., Huang, Y., Kang, L., Warner, C. A., Xie, H., Chen, L., & Duan, X. (2023) · World Journal of Gastroenterology · doi:10.3748/wjg.v29.i9.1446 · Cited by 20 · see citing works
Ma, C., Shi, S.-R., Zhang, X., Xin, G., Zou, X., Li, W., & Guo, S. (2024) · Biomolecules · doi:10.3390/biom14111446 · Cited by 19 · see citing works
Zheng, Y., Ji, S., Li, X., & Wen, L. (2023) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2023.115794 · Cited by 16 · see citing works
Dong, Z., Guan, H., Wang, L., Liang, L., Zang, Y., Wu, L., & Bao, L. (2024) · Molecular Medicine Reports · doi:10.3892/mmr.2024.13314 · Cited by 16 · see citing works
Self-citationLuo, L., Chen, Y., Fuchi, N., Kodama, Y., Zhang, X., Goto, S., Miura, K., Sasaki, H., & Li, T. (2021) · Stem Cell Research & Therapy · doi:10.1186/s13287-021-02504-5 · Cited by 13 · see citing works
MicroRNAs in organ fibrosis: From molecular mechanisms to potential therapeutic targets
Zhao, H., Feng, Y., Liu, T., Wang, J., & Yu, J. (2021) · Pathology - Research and Practice · doi:10.1016/j.prp.2021.153588 · Cited by 13 · see citing works
The roles of long noncoding RNA-mediated macrophage polarization in respiratory diseases
Qiao, X., Ding, Y., Wu, D., Zhang, A., Yin, Y., Wang, Q., Wang, W. T., & Kang, J. (2023) · Frontiers in Immunology · doi:10.3389/fimmu.2022.1110774 · Cited by 12 · see citing works
Phosphatase and Tensin Homolog in Non-neoplastic Digestive Disease: More Than Just Tumor Suppressor
He, T., Zhang, X., Hao, J., & Ding, S. (2021) · Frontiers in Physiology · doi:10.3389/fphys.2021.684529 · Cited by 10 · see citing works
Sex‐specific analysis of microRNA profiles in HBV‐associated cirrhosis by small RNA‐sequencing
Chan, K. K., Au, K. K., Fung, W., Wong, C., Chan, A. C. Y., & Lo, R. C. (2022) · Hepatology Communications · doi:10.1002/hep4.2096 · Cited by 8 · see citing works
Bao, L., Chu, Y., & Kang, H. (2023) · Genomics · doi:10.1016/j.ygeno.2023.110597 · Cited by 7 · see citing works
Self-citationNie, K., Liu, L., Peng, L., Zhang, M., Zhang, C., Xiao, B., Xia, Z., & Huang, W. J. (2022) · Journal of Molecular Neuroscience · doi:10.1007/s12031-022-01971-6 · Cited by 7 · see citing works
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Sulaiman, S. A., Dorairaj, V., Ghafar, K. N. A., & Murad, N. A. A. (2021) · Livers · doi:10.3390/livers1040021 · Cited by 6 · see citing works
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Sohn, E. J., & Oh, S.-O. (2026) · Analytical Cellular Pathology · doi:10.1155/ancp/2283163 · Cited by 1 · see citing works
- Authors
- Dong, Z., Li, S., Si, L., Ma, R., Bao, L., & Bo, A.
- Affiliations
- Inner Mongolia Medical University (CN)
- Baotou Medical College (CN)
- Corresponding author
- Bao, Lidao
- Bo, Agula
- Venue
- European Journal of Clinical Investigation
- Publisher
- Wiley
- Published
- 2020-05-15
- Volume
- 50
- Issue
- 6
- Pages
- e13243-e13243
- ISSN
- 0014-2972 (ISSN-L)
- 1365-2362
- OpenAlex
- W3016549002
- Cited by
- 18
- References
- 42
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 3 · 2023: 4 · 2022: 3 · 2021: 5
- FWCI
- 1.76
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Clinical Investigation (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (81550047, 81760748)
- Topics
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Health Sciences › Medicine › Epidemiology › Liver Disease Diagnosis and Treatment
- Keywords
- liver fibrosis, miR-23a-5p, hepatic stellate cell activation, PTEN, platelet-derived growth factor, therapeutic target
- MeSH terms
- Animals, Cell Line, Dimethylnitrosamine, Liver Cirrhosis, Blotting, Western, Phosphatidylinositol 3-Kinases, MicroRNAs, Proto-Oncogene Proteins c-akt, PTEN Phosphohydrolase, Rats, Hepatic Stellate Cells, Gene Knockdown Techniques, Real-Time Polymerase Chain Reaction, RNA, Long Noncoding
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