The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Efficient discovery of DNA polymorphisms in natural populations by Ecotilling
Abstract
Summary: We have adapted the mutation detection technology used in Targeting Induced Local Lesions in Genomes (TILLING) to the discovery of polymorphisms in natural populations. The genomic DNA of a queried individual is mixed with a reference DNA and used to amplify a target 1‐kbp region of DNA with asymmetrically labeled fluorescent primers. After heating and annealing, heteroduplexes are nicked at mismatched sites by the endonuclease CEL I and cut strands are visualized using Li‐cor gel analyzers. Putative polymorphisms detected in one fluorescence channel can be verified by appearance of the opposite cut strand in the other channel. We demonstrated the efficiency of this technology, called Ecotilling, by the discovery in 150+ individuals of 55 haplotypes in five genes, ranging from sequences differing by a single nucleotide polymorphism to those representing complex haplotypes. The discovered polymorphisms were confirmed by sequencing and included base‐pair changes, small insertions and deletions, and variation in microsatellite repeat number. Ecotilling allows the rapid detection of variation in many individuals and is cost effective because only one individual for each haplotype needs to be sequenced. The technology is applicable to any organism including those that are heterozygous and polyploid.
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Cites
Mathematical model for studying genetic variation in terms of restriction endonucleases.
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Patterns of linkage disequilibrium in the human genome
Ardlie, K. G., Kruglyak, L., & Seielstad, M. (2002) · Nature Reviews Genetics · doi:10.1038/nrg777 · Cited by 1,129
Applications of single nucleotide polymorphisms in crop genetics
Rafalski, A. (2002) · Current Opinion in Plant Biology · doi:10.1016/s1369-5266(02)00240-6 · Cited by 1,066
T argeting I nduced L ocal L esions IN G enomes (TILLING) for Plant Functional Genomics
McCallum, C. M., Comai, L., Greene, E. A., & Henikoff, S. (2000) · Plant Physiology · doi:10.1104/pp.123.2.439 · Cited by 875
Self-citationRole of the Arabidopsis DRM Methyltransferases in De Novo DNA Methylation and Gene Silencing
Cao, X., & Jacobsen, S. E. (2002) · Current Biology · doi:10.1016/s0960-9822(02)00925-9 · Cited by 791
Targeted screening for induced mutations
McCallum, C. M., Comai, L., Greene, E. A., & Henikoff, S. (2000) · Nature Biotechnology · doi:10.1038/74542 · Cited by 744
Self-citationDenaturing high-performance liquid chromatography: A review
Xiao, W., & Oefner, P. J. (2001) · Human Mutation · doi:10.1002/humu.1130 · Cited by 725
Spectrum of Chemically Induced Mutations From a Large-Scale Reverse-Genetic Screen in Arabidopsis
Greene, E. A., Codomo, C. A., Taylor, N. E., Henikoff, J. G., Till, B. J., Reynolds, S. H., Enns, L. C., Burtner, C., Johnson, J. E., Odden, A. R., et al. (2003) · Genetics · doi:10.1093/genetics/164.2.731 · Cited by 640
Self-citationLarge-Scale Discovery of Induced Point Mutations With High-Throughput TILLING
Till, B. J., Reynolds, S. H., Greene, E. A., Codomo, C. A., Enns, L. C., Johnson, J. E., Burtner, C., Odden, A. R., Young, K., Taylor, N. E., et al. (2003) · Genome Research · doi:10.1101/gr.977903 · Cited by 607
Self-citationMethods for Genotyping Single Nucleotide Polymorphisms
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Research works printed but not linked: 6
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Cited by
Enhancing genetic gain in the era of molecular breeding
Xu, Y., Li, P., Zou, C., Lu, Y., Xie, C., Zhang, X., Prasanna, B. M., & Olsen, M. S. (2017) · Journal of Experimental Botany · doi:10.1093/jxb/erx135 · Cited by 336
Teichmann, T., & Muhr, M. (2015) · Frontiers in Plant Science · doi:10.3389/fpls.2015.00233 · Cited by 228
“A variant of this technique, called Ecotilling (Comai et al., 2004), could be used to screen natural populations for desired polymorphisms in order to exploit natural variation for breeding.”
MethodTriques, K., Sturbois, B., Gallais, S., Dalmais, M., Chauvin, S., Clepet, C., Aubourg, S., Rameau, C., Caboche, M., & Bendahmane, A. (2007) · The Plant Journal · doi:10.1111/j.1365-313x.2007.03201.x · Cited by 145
Metadata
- Authors
- Comai, L., Young, K., Till, B. J., Reynolds, S. H., Greene, E. A., Codomo, C. A., Enns, L. C., Johnson, J. E., Burtner, C., Odden, A. R., & Henikoff, S.
- Venue
- The Plant Journal
- Publisher
- Wiley
- Published
- 2004-02-10
- Volume
- 37
- Issue
- 5
- Pages
- 778-786
- ISSN
- 0960-7412 (ISSN-L)
- 1365-313X
- OpenAlex
- W1996433825
- Cited by
- 451
- References
- 32
- FWCI
- 35.99
- Type
- journal article
- Language
- en
- Open access
- closed
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