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Cited by 78Bacterial Genetics and BiotechnologyAntimicrobial Resistance in StaphylococcusHeavy Metal Pollution Remediation

Chromosome‐Determined Zinc‐Responsible Operon czr in Staphylococcus aureus Strain 912

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Kuroda, Makoto, Hayashi, Hideo, Ohta, Toshiko·1999·Microbiology and Immunology
DOI 10.1111/j.1348-0421.1999.tb02382.x
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Abstract

A novel operon, czrAB (zinc‐responsible genes), was identified in the chromosome of Staphylococcus aureus. The operon consists of two genes, czrA and czrB. The czrA gene, coding for an 11.5 kDa protein, was homologous to cadC, arsR of S. aureus plasmid pI258 and smtB of Synechococcus PCC7942. The czrB, coding for a 36 kDa membrane spanning protein, was homologous to the czcD gene, cobalt, zinc and the cadmium‐resistant factor of Bacillus subtilis and Alcaligenes eutrophus. In the presence of zinc (0.1‐10 mM), the transcription of czrAB was enhanced in a concentration‐dependent manner. Other heavy metals, such as cobalt, copper, manganese and nickel showed no effect on czrAB expression. The disruptant of the czrB gene became sensitive to zinc ion (MIC, 2 mM; MBC, 10 mM), and the complementation with the plasmid recovered the resistance to zinc at the same concentration as a parental strain (MIC, 5 mM; MBC, 20 mM). The disruptant accumulated intracellular zinc up to 0.4 mg per g dry weight of the organism, while that of the parental strain was 0.25 mg per g dry weight. The findings indicated that the novel operon czrAB should play a role in the transportation of zinc across the cell membrane to maintain the proper intracellular concentration.

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  • Czc-type exporters have been postulated to mediate zinc efflux in other bacteria, including P. aeruginosa and S. aureus.

    “Czc-type exporters have subsequently been postulated to mediate efflux of zinc in other bacteria, including P. aeruginosa and S. aureus [146, 202].”

    From Metal ion acquisition in Staphylococcus aureus: overcoming nutritional immunity (2012) · cites this paper as [146]

  • Resistance to both zinc and cobalt is conferred by the chromosomally encoded czrAB operon in S. aureus.

    “Resistance to both zinc and cobalt is conferred by the chromosomally encoded czrAB operon, which encodes a metal-regulated transcriptional repressor (CzrA, also known as ZntR) and a CDF antiporter (CzrB, also known as ZntA) [146, 147, 203].”

    From Metal ion acquisition in Staphylococcus aureus: overcoming nutritional immunity (2012) · cites this paper as [146]

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Authors
Kuroda, M., Hayashi, H., & Ohta, T.
Affiliations
University of Tsukuba (JP)
Corresponding author
Ohta, Toshiko
ORCID
Kuroda, Makoto
Venue
Microbiology and Immunology
Publisher
Wiley
Published
1999-02
Volume
43
Issue
2
Pages
115-125
ISSN
  • 0385-5600 (ISSN-L)
  • 1348-0421
DOI
10.1111/j.1348-0421.1999.tb02382.x
OpenAlex
W2041401296
Cited by
78
References
46
Citations by year
2026: 3 · 2025: 2 · 2024: 4 · 2023: 1 · 2021: 2 · 2020: 2 · 2019: 1 · 2018: 2 · 2017: 3 · 2016: 1 · 2015: 2 · 2014: 2 · 2013: 2 · 2012: 5
FWCI
2.11
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 5400 d)
Locations
  • Microbiology and Immunology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
  • Physical Sciences › Environmental Science › Water Science and Technology › Heavy Metal Pollution Remediation
Keywords
Staphylococcus aureus, zinc resistance, zinc transport, transcriptional regulation, gene disruption, complementation, heavy metal resistance
MeSH terms
Amino Acid Sequence, Bacterial Proteins, Base Sequence, Chromosomes, Bacterial, DNA-Binding Proteins, DNA, Bacterial, Genetic Complementation Test, Humans, Membrane Proteins, Metals, Molecular Sequence Data, Operon, Staphylococcus aureus, Transcription, Genetic, Zinc, Mutagenesis, Sequence Homology, Amino Acid

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