Random Segregation of DNA Strands in Epidermal Basal Cells
Open accessAbstract
According to the hypothesis proposed by Cairns, stem cells retain the older of the two parental DNA strands, whereas differentiating daughter cells receive the newly synthesized strand, so that a set of “immortal strands” persists in stem cells through successive cell divisions. To test this hypothesis, five successive divisions were induced in basal epidermal cells in vivo by two injections of cholera toxin into mouse skin and cells labeled with [3 H]thymidine at the first cell cycle were chased for 50 days. If selective segregation occurs, the labeled strand should be transferred into a non‐stem daughter cell after the second division and labeled cells would eventually be eliminated from the epidermis. However, the results suggest random segregation of DNA strands in epidermal basal cells. Labeled basal cells were persistently present throughout the whole epidermis for 50 days. Furthermore, labeled mitotic cells were found after the third division and their numbers of grains decreased exponentially through 5 cycles of divisions.
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Evidence of asymmetric chromosome segregation was lacking in certain studies of epidermal stem cells and ISCs.
“evidence of asymmetric chromosome segregation was lacking in certain studies of epidermal stem cells136,137 and ISCs138.”
From Molecular regulation of stem cell quiescence (2013) · cites this paper as [137]
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Cites
Mutation selection and the natural history of cancer
Cairns, J. (1975) · Nature · doi:10.1038/255197a0 · Cited by 1,592 · see citing works
Lajtha, L. G. (1979) · Differentiation · doi:10.1111/j.1432-0436.1979.tb01007.x · Cited by 558 · see citing works
Identification and Behavior of Label-retaining Cells in Oral Mucosa and Skin
Bickenbach, J. R. (1981) · Journal of Dental Research · doi:10.1177/002203458106000311011 · Cited by 320 · see citing works
The segregation of DNA in epithelial stem cells
Potten, C. S., Hume, W. J., Reid, P. J., & Cairns, J. (1978) · Cell · doi:10.1016/0092-8674(78)90274-x · Cited by 313 · see citing works
A clonal marker induced by mutation in mouse intestinal epithelium
Winton, D. J., Blount, M., & Ponder, B. A. J. (1988) · Nature · doi:10.1038/333463a0 · Cited by 263 · see citing works
Morris, R., Fischer, S. M., & Slaga, T. J. (1985) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12265358 · Cited by 173 · see citing works
Demonstration of somatic mutation and colonic crypt clonality by X-linked enzyme histochemistry
Griffiths, D. F. R., Davies, S. J., Williams, D. W., Williams, G., & Williams, E. D. (1988) · Nature · doi:10.1038/333461a0 · Cited by 170 · see citing works
Identification and Localization of Label-Retaining Cells in Hamster Epithelia
Bickenbach, J. R., & Mackenzie, I. C. (1984) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12261460 · Cited by 123 · see citing works
Rate of Loss of Tritiated Thymidine Label In Basal Cells In Mouse epithelial tissues
Bickenbach, J. R., McCutecheon, J., & Mackenzie, I. C. (1986) · Cell Proliferation · doi:10.1111/j.1365-2184.1986.tb00684.x · Cited by 96 · see citing works
Stem cells: Their identification and characterisation
(1984) · Food and Chemical Toxicology · doi:10.1016/0278-6915(84)90179-0 · Cited by 62 · see citing works
Stenbäck, F., Pető, R., & Shubik, P. (1981) · British Journal of Cancer · doi:10.1038/bjc.1981.141 · Cited by 56 · see citing works
Kuroki, T. (1981) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.78.11.6958 · Cited by 30 · see citing works
Self-citationCholera Toxin as a Mitotic Stimulator of Epidermal Cells in vitro and in vivo
Kuroki, T. (1985) · Springer eBooks · doi:10.1007/978-94-009-4990-4_16 · Cited by 3 · see citing works
Self-citationPotten, C. S. (1982) · Cellular and Molecular Life Sciences · doi:10.1007/bf01955773 · Cited by 1 · see citing works
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Molecular regulation of stem cell quiescence
Cheung, T. H., & Rando, T. A. (2013) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3591 · Cited by 1,148 · see citing works
Asymmetric division and cosegregation of template DNA strands in adult muscle satellite cells
Shinin, V., Gayraud-Morel, B., Gomès, D., & Tajbakhsh, S. (2006) · Nature Cell Biology · doi:10.1038/ncb1425 · Cited by 504 · see citing works
Sotiropoulou, P. A., Candi, A., & Blanpain, C. (2008) · Stem Cells · doi:10.1634/stemcells.2008-0634 · Cited by 67 · see citing works
Stem cell ageing and non-random chromosome segregation
Charville, G. W., & Rando, T. A. (2011) · Philosophical Transactions of the Royal Society B: Biological Sciences · doi:10.1098/rstb.2010.0279 · Cited by 45 · see citing works
- Authors
- Kuroki, T., & Murakami, Y.
- Affiliations
- Institute of Medical Science, The University of Tokyo (JP)
- Venue
- Japanese Journal of Cancer Research
- Publisher
- Wiley
- Published
- 1989-07
- Volume
- 80
- Issue
- 7
- Pages
- 637-642
- ISSN
- 0910-5050 (ISSN-L)
- PMC
- PMC5917816
- OpenAlex
- W1982813112
- Cited by
- 27
- References
- 14
- Citations by year
- 2024: 1 · 2018: 1 · 2017: 1 · 2015: 1 · 2013: 4 · 2012: 2
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Japanese Journal of Cancer Research (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Health Sciences › Medicine › Urology › Hair Growth and Disorders
- Keywords
- epidermal basal cells, stem cells, DNA synthesis, 3H-thymidine labeling, cholera toxin, cell cycle, mouse skin
- MeSH terms
- Epidermal Cells, Animals, Autoradiography, Cell Compartmentation, Cell Division, Cholera Toxin, DNA, Female, Mitosis, Stem Cells, Mice
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