Cited by 155NF-κB Signaling PathwaysUbiquitin and proteasome pathwaysTGF-β signaling in diseases

VCP (p97) Regulates NFKB Signaling Pathway, Which Is Important for Metastasis of Osteosarcoma Cell Line

Open access
Asai, Tatsuya, Tomita, Yasuhiko, Nakatsuka, Shin‐ichi, Hoshida, Yoshihiko, Myoui, Akira, Yoshikawa, Hideki, Aozasa, Katsuyuki·2002·Japanese Journal of Cancer Research

Abstract

In order to identify genes associated with metastasis, suppression subtractive hybridization (SSH) was performed using murine osteosarcoma cell line Dunn and its subline with higher metastatic potential, LM8. SSH revealed expression of the gene encoding valosin‐containing protein (VCP; also known as p97) to be constitutively activated in LM8 cells, but it declined in Dunn cells when the cells became confluent. Because VCP is known to be involved in the ubiquitination process of Inhibitor‐αBα (IαBα), an inhibitor of nuclear factor‐αB (NFαB), whether VCP influences NFαB activation or not was examined by using VCP‐transfected Dunn cells (Dunn/VCPs). When stimulated with tumor necrosis factor‐α (TNFα), Dunn/VCPs showed constantly activated NFαB, although in the original Dunn cells and control vector transfectant (Dunn/Dunn‐c) NFαB activation ceased when the cells became confluent. Western immunoblot analysis showed an increase of phosphorylated IαBα (p‐IKBα) in the cytoplasm of confluent Dunn/Dunn‐c cells compared to that of Dunn/VCPs. Therefore, decrease of p‐IKBα degrading activity might be responsible for the decrease in NFKB activation. In vitro apoptosis assay demonstrated increased apoptosis rates of Dunn/Dunn‐c cells after TNFα stimulation compared to those of Dunn/VCPs and LM8 cells. In vivo metastasis assay showed increased incidences of metastatic events in Dunn/VCP‐1 inoculated male C3H mice compared to those in Dunn/Dunn‐c inoculated mice. These findings suggested that VCP expression plays an important role in the metastatic process. Anti‐apoptotic potential in these cells owing to constant NFKB activation via efficient cytoplasmic p‐IKBα degrading activity may explain the increased metastatic potential of these cells.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1111/j.1349-7006.2002.tb02172.x

Cites

46 of 46 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • ALS-associated mutations in TBK1, OPTN, SQSTM1, and VCP all affect autophagy as well as microglial function and innate immunity.

    “ALS-associated mutations in TBK1, OPTN, SQSTM1, and VCP all affect autophagy as well as microglial function and innate immunity [192,205,206,441–443].”

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Asai, T., Tomita, Y., Nakatsuka, S., Hoshida, Y., Myoui, A., Yoshikawa, H., & Aozasa, K.
Affiliations
The University of Osaka (JP)
Corresponding author
Tomita, Yasuhiko
Venue
Japanese Journal of Cancer Research
Publisher
Wiley
Published
2002-03
Volume
93
Issue
3
Pages
296-304
ISSN
0910-5050 (ISSN-L)
OpenAlex
W1994246241
Cited by
155
References
46
Citations by year
2026: 4 · 2025: 1 · 2024: 4 · 2022: 15 · 2021: 6 · 2020: 4 · 2019: 7 · 2018: 2 · 2017: 3 · 2016: 4 · 2015: 7 · 2014: 5 · 2013: 17 · 2012: 6
FWCI
3.45
Type
journal article
Language
en
Open access
Locations
  • Japanese Journal of Cancer Research (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
Japan Society for the Promotion of Science
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › NF-κB Signaling Pathways
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › TGF-β signaling in diseases
Keywords
VCP, osteosarcoma, metastasis, NF-κB signaling, IκBα phosphorylation, ubiquitination, TNF-α, apoptosis, cell confluence
MeSH terms
Valosin Containing Protein, Adenosine Triphosphatases, Animals, Bone Neoplasms, Gene Expression Regulation, Humans, Lung Neoplasms, Male, Mice, Inbred C3H, Microscopy, Fluorescence, Plasmids, Osteosarcoma, Transfection, Tumor Cells, Cultured, Tumor Necrosis Factor-alpha, Blotting, Northern, Signal Transduction, NF-kappa B, Apoptosis, DNA Primers, Cell Cycle Proteins, Reverse Transcriptase Polymerase Chain Reaction, Electrophoretic Mobility Shift Assay, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review