Identification of H‐ and L‐ferritin subunit binding sites on human T and B lymphoid cells
Abstract
Summary: Monoclonal antibodies to the H and L subunits of ferritin were used in indirect immunofluorescent studies to investigate the subunit specificity of ferritin binding to transformed T and B lymphoid cell lines. Pre‐incubation of cells with recombinant H ferritin followed by antibodies to the H subunit produced a labelling pattern in which all cells showed strong fluorescent stippling. Corresponding experiments with liver ferritin and an antibody to the L subunit revealed that most cells were lightly stippled, but 3–5% of the cells were intensely labelled. These two labelling patterns were distinct as assessed by cross‐blocking experiments with recombinant H and liver ferritins. These results imply that different binding sites exist for each subunit.
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Binding of ferritin L to the cell surface of liver, T-, and B-lymphoid cells was demonstrated.
“binding of ferritin L to the cell surface of liver, T-, and B-lymphoid cells was demonstrated (7, 208)”
From Intracellular iron transport and storage: from molecular mechanisms to health implications (2008) · cites this paper as [7]
Cites
FERRITIN: STRUCTURE, GENE REGULATION, AND CELLULAR FUNCTION IN ANIMALS, PLANTS, AND MICROORGANISMS
Theil, E. C. (1987) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.56.070187.001445 · Cited by 1,337 · see citing works
On ferritin heterogeneity. Further evidence for heteropolymers.
Arosio, P., Adelman, T. G., & Drysdale, J. (1978) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)34741-5 · Cited by 457 · see citing works
Self-citationSibille, J., Kondo, H. G., & Aisen, P. (1988) · Hepatology · doi:10.1002/hep.1840080218 · Cited by 139 · see citing works
NADH diferric transferrin reductase in liver plasma membrane.
SUN, I. L., Navas, P., Crane, F. L., et al. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)47676-4 · Cited by 133 · see citing works
Bomford, A. B., Conlon-Hollingshead, C., & Munro, H. N. (1981) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)70071-4 · Cited by 129 · see citing works
Iron metabolism in the erythrophagocytosing Kupffer cell
Kondo, H. G., Saito, K., Grasso, J. P., & Aisen, P. (1988) · Hepatology · doi:10.1002/hep.1840080108 · Cited by 125 · see citing works
Detection and isolation of a hepatic membrane receptor for ferritin.
Mack, U., Powell, L. W., & Halliday, J. W. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)32470-0 · Cited by 108 · see citing works
Self-citationBroxmeyer, H., Bognacki, J. J., Ralph, P., Dörner, M., Lu, L., & Castro-Malaspina, H. R. (1982) · Blood · doi:10.1182/blood.v60.3.595.bloodjournal603595 · Cited by 100 · see citing works
Immunochemical characterization of human liver and heart ferritins with monoclonal antibodies
Luzzago, A., Arosio, P., Iacobello, C., Ruggeri, G., Capucci, L., Brocchi, E., Simone, F., Gamba, D., Gabri, E., Levi, S., et al. (1986) · Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology · doi:10.1016/0167-4838(86)90147-0 · Cited by 93 · see citing works
Self-citationLu, L., Broxmeyer, H., Meyers, P., et al. (1983) · Blood · doi:10.1182/blood.v61.2.250.250 · Cited by 88 · see citing works
Recent Developments in Immunofluorescence
Faulk, W. P., & Hijmans, W. (2015) · Chemical immunology/Fortschritte der Allergielehre/Progress in allergy/Chemical immunology and allergy · doi:10.1159/000393067 · Cited by 86 · see citing works
Self-citationMoroz, C., Lahat, N., Biniaminov, M., & Ramot, B. (1977) · PubMed Central · Cited by 81 · see citing works
Removal of HLA-A,B antigens from platelets
Blumberg, N., Masel, D., Mayer, T., et al. (1984) · Blood · doi:10.1182/blood.v63.2.448.448 · Cited by 81 · see citing works
Hepatocellular Uptake of Ferritin in the Rat
Unger, A., & Hershko, C. (1974) · British Journal of Haematology · doi:10.1111/j.1365-2141.1974.tb06651.x · Cited by 78 · see citing works
Isolation of a human hepatic ferritin receptor
Adams, P. C., Powell, L. W., & Halliday, J. W. (1988) · Hepatology · doi:10.1002/hep.1840080402 · Cited by 76 · see citing works
Self-citationDistribution of ferritin receptors and coated pits on giant HeLa cells.
Bretscher, M. S., & Thomson, J. N. (1983) · The EMBO Journal · doi:10.1002/j.1460-2075.1983.tb01469.x · Cited by 69 · see citing works
Characteristics and expression of binding sites specific for ferritin H- chain on human cell lines
Fargion, S. R., Arosio, P., Fracanzani, A. L., Cislaghi, V., Levi, S., Cozzi, A., Piperno, A., & Fiorelli, G. (1988) · Blood · doi:10.1182/blood.v71.3.753.753 · Cited by 64 · see citing works
Self-citationTransferrin Receptor Expression and the Control of Cell Growth
Neckers, L. Μ., & Trepel, J. B. (1986) · Cancer Investigation · doi:10.3109/07357908609017524 · Cited by 61 · see citing works
Yeh, C. G., Papamichael, M., & Faulk, W. P. (1982) · Experimental Cell Research · doi:10.1016/0014-4827(82)90192-6 · Cited by 60 · see citing works
Self-citationDifferential effect of isolated placental isoferritins on in vitro T‐lymphocyte function
Matzner, Y., Konijn, A. M., Shlomai, Z., & Ben‐Bassat, H. (1985) · British Journal of Haematology · doi:10.1111/j.1365-2141.1985.tb07331.x · Cited by 58 · see citing works
The Kinetics of Serum and Tissue Ferritins: Relation to Carbohydrate Content
Halliday, J. W., Mack, U., & Powell, L. W. (1979) · British Journal of Haematology · doi:10.1111/j.1365-2141.1979.tb01166.x · Cited by 58 · see citing works
Self-citationIn vitro suppression of cell-mediated immunity by ferroproteins and ferric salts
Keown, P. A., & Descamps‐Latscha, B. (1983) · Cellular Immunology · doi:10.1016/0008-8749(83)90114-4 · Cited by 53 · see citing works
Löw, H., Grebing, C., Lindgren, A., et al. (1987) · Journal of Bioenergetics and Biomembranes · doi:10.1007/bf00770036 · Cited by 47 · see citing works
Human ferritin H and L sequences lie on ten different chromosomes
McGill, J., Naylor, S. L., Sakaguchi, A. Y., Moore, C., Boyd, D. H., Barrett, K., Shows, T. B., & Drysdale, J. (1987) · Human Genetics · doi:10.1007/bf00283053 · Cited by 46 · see citing works
Oncofoetal antigens of human trophoblast
Faulk, W. P., Yeager, C., McIntyre, J. A., et al. (1979) · Proceedings of the Royal Society B Biological Sciences · doi:10.1098/rspb.1979.0099 · Cited by 43 · see citing works
Self-citationKao, K.-J. (1988) · Transfusion · doi:10.1046/j.1537-2995.1988.28188127942.x · Cited by 40 · see citing works
Ferritin and iron uptake by reticulocytes
Blight, G. D., & Morgan, E. H. (1983) · British Journal of Haematology · doi:10.1111/j.1365-2141.1983.tb01224.x · Cited by 38 · see citing works
Characterization of the binding of ferritin to the rat liver ferritin receptor
Mack, U., Storey, E., Powell, L. W., & Halliday, J. W. (1985) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/0304-4165(85)90135-7 · Cited by 37 · see citing works
Self-citationHuman placental ferritin receptor
Takami, M., Mizumoto, K., Kasuya, I., Kino, K., Sussman, H. H., & Tsunoo, H. (1986) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/0304-4165(86)90223-0 · Cited by 36 · see citing works
Immunobiology of membrane-bound collagen on mouse fibroblasts
Faulk, W. P., Conochie, L. B., Temple, A., et al. (1975) · Nature · doi:10.1038/256123a0 · Cited by 36 · see citing works
Self-citationRecent Developments in Immunofluorescence (with colour plates I and II)
Faulk, P., & Hijmans, W. (1972) · Chemical immunology/Fortschritte der Allergielehre/Progress in allergy/Chemical immunology and allergy · doi:10.1159/000313167 · Cited by 34 · see citing works
Immature red cells have ferritin receptors
Pollack, S., & Campana, T. (1981) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(81)90710-5 · Cited by 34 · see citing works
Transcription of T7 DNA containing modified nucleotides by bacteriophage T7 specific RNA polymerase.
Stahl, S. J., & Chamberlin, M. J. (1978) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)34640-9 · Cited by 31 · see citing works
Ferritin-bearing lymphocytes and T-cell levels in peripheral blood of patients with breast cancer
Moroz, C., Giler, S., Kupfer, B., et al. (1977) · Cancer Immunology Immunotherapy · doi:10.1007/bf00200066 · Cited by 30 · see citing works
Effect of acidic and basic isoferritins on in vitro growth of human granulocyte-monocyte progenitors
Dezza, L., Cazzola, M., Piacibello, W., et al. (1986) · Blood · doi:10.1182/blood.v67.3.789.789 · Cited by 30 · see citing works
Ferritin-bearing lymphocytes in patients with cancer
Papenhausen, P. R., Emeson, E. E., Croft, C. B., et al. (1984) · Cancer · doi:10.1002/1097-0142(19840115)53:2 · Cited by 15 · see citing works
Specific binding of acidic isoferritins to erythroleukemia K562 cells.
Am, C., De, E., Bs, S., et al. (1987) · PubMed · Cited by 13 · see citing works
Ciriello, M. M., Cazzola, M., Dezza, L., et al. (1987) · Tumori Journal · doi:10.1177/030089168707300107 · Cited by 7 · see citing works
Faulk, W. P., Torry, D. S., HARATS, H., McIntyre, J. A., & Taylor, C. G. (1988) · Springer eBooks · doi:10.1007/978-1-4684-8029-0_20 · Cited by 6 · see citing works
Self-citationEffect of acidic and basic isoferritins on in vitro growth of human granulocyte-monocyte progenitors
Dezza, L., Cazzola, M., Piacibello, W., et al. (1986) · Blood · doi:10.1182/blood.v67.3.789.bloodjournal673789 · Cited by 4 · see citing works
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Intracellular iron transport and storage: from molecular mechanisms to health implications
Mackenzie, E. L., Iwasaki, K., & Tsuji, Y. (2008) · Antioxidants & Redox Signaling · doi:10.1089/ars.2007.1893 · Cited by 526 · see citing works
TIM-2 is expressed on B cells and in liver and kidney and is a receptor for H-ferritin endocytosis
Chen, T. T., Li, L., Chung, D.-H., Allen, C. D. C., Torti, S. V., Torti, F. M., Cyster, J. G., Chen, C.-Y., Brodsky, F. M., Niemi, E. C., et al. (2005) · The Journal of Experimental Medicine · doi:10.1084/jem.20042433 · Cited by 219 · see citing works
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Kwok, J. C., & Richardson, D. R. (2002) · Critical Reviews in Oncology/Hematology · doi:10.1016/s1040-8428(01)00213-x · Cited by 215 · see citing works
- Authors
- Anderson, G. J., Faulk, W. P., Arosio, P., Moss, D., Powell, L. W., & Halliday, J. W.
- Affiliations
- The University of Queensland (AU)
- University of Milan (IT)
- Corresponding author
- Halliday, J. W.
- Venue
- British Journal of Haematology
- Publisher
- Wiley
- Published
- 1989-10
- Volume
- 73
- Issue
- 2
- Pages
- 260-264
- ISSN
- 0007-1048 (ISSN-L)
- 1365-2141
- OpenAlex
- W2065433626
- Cited by
- 23
- References
- 40
- Citations by year
- 2014: 1
- FWCI
- 0.47
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- British Journal of Haematology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Health and Medical Research Council
- National Medical Research Council
- Topics
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Hemoglobin structure and function
- Life Sciences › Neuroscience › Neurology › Neurological diseases and metabolism
- Keywords
- ferritin, H subunit, L subunit, T cells, B cells, lymphoid cell lines, monoclonal antibodies, liver ferritin
- MeSH terms
- Antibodies, Monoclonal, B-Lymphocytes, Binding Sites, Burkitt Lymphoma, Cell Line, Ferritins, Fluorescent Antibody Technique, Humans, Receptors, Cell Surface, T-Lymphocytes, Tumor Cells, Cultured, Iron-Binding Proteins, Precursor Cell Lymphoblastic Leukemia-Lymphoma
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