The role of lipids in the detection of lupus anticoagulant by the dilute Russell Viper venom test: are platelets or reagents containing hexagonal HII phases necessary?
Abstract
Liposomes prepared from rabbit brain extracts (RBE) and individual pure lipids (high phosphatidyl serine content, HIPS) were compared with frozen-thawed platelets (PLTS) in the dilute Russell Viper venom time (dRVVt). While all three preparations demonstrated sensitivity to lupus anticoagulant (LA) the highest detection rate was seen with RBE. For confirmation of LA, high concentration RBE achieved the most efficient correction of the defect. Electron microscopy and particle sizing showed RBE to be small, discrete liposomes, whereas HIPS and PLTS were aggregates of larger diameter particles. Low-angle X-ray diffraction showed no evidence of hexagonal HII phase. There appears to be no specific requirement either for platelets or for reagents containing hexagonal HII phases in the dRVVt. The dRVVt can be optimized by incorporating a simple dilute rabbit brain lipid mixture for detection of LA and a concentrated mixture as a correcting reagent.
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The higher the relative content of phosphatidylserine, the less the sensitivity of the assay to LA.
“the higher the relative content of phosphatydilserine, the less the sensitivity of the assay to LA.”
From Lupus Anticoagulant Detection under the Magnifying Glass (2023) · cites this paper as [43]
Cites
Kelsey, P. R., Stevenson, K. J., & Poller, L. (1984) · Thrombosis and Haemostasis · doi:10.1055/s-0038-1661166 · Cited by 91 · see citing works
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Moore, G. W., & Savidge, G. F. (2006) · Thrombosis Research · doi:10.1016/j.thromres.2005.09.010 · Cited by 46 · see citing works
Lupus Anticoagulant Detection under the Magnifying Glass
Molinari, A. C., Martini, T., Banov, L., Ierardi, A., Leotta, M., Strangio, A., & Santoro, R. C. (2023) · Journal of Clinical Medicine · doi:10.3390/jcm12206654 · Cited by 9 · see citing works
Analytical dilemmas in lupus anticoagulant detection
Moore, G. W. (2023) · Exploration of Immunology · doi:10.37349/ei.2023.00104 · Cited by 5 · see citing works
- Authors
- Stevenson, K. J., & Seddon, J. M.
- Affiliations
- Withington Community Hospital (GB)
- Imperial College London (GB)
- Corresponding author
- Stevenson, K. J.
- ORCID
- Seddon, J. M.
- Venue
- British Journal of Haematology
- Publisher
- Wiley
- Published
- 1994-03
- Volume
- 86
- Issue
- 3
- Pages
- 583-589
- ISSN
- 0007-1048 (ISSN-L)
- 1365-2141
- OpenAlex
- W2013259888
- Cited by
- 22
- References
- 1
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 4 · 2022: 1 · 2013: 1
- FWCI
- 0.97
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- British Journal of Haematology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Rheumatology › Systemic Lupus Erythematosus Research
- Health Sciences › Medicine › Hematology › Blood Coagulation and Thrombosis Mechanisms
- Life Sciences › Immunology and Microbiology › Immunology › Complement system in diseases
- Keywords
- lupus anticoagulant, dilute Russell viper venom time, liposomes, particle size, low-angle X-ray diffraction
- MeSH terms
- Animals, Blood Platelets, Brain, Humans, Indicators and Reagents, Liposomes, Microscopy, Electron, Phospholipids, Prothrombin Time, Rabbits, Tissue Extracts, Lupus Coagulation Inhibitor
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