Usefulness of Synthetic Phospholipid in Measurement of Activated Partial Thromboplastin Time: A New Preparation Procedure to Reduce Batch Difference
Abstract
Commercial activated partial thromboplastin time (APTT) reagents prepared with phospholipid extracted from animal or plant sources often differ in their response to heparin and coagulation factors and in their reference values. It is also known that there are variations in phospholipid composition and preparation procedure. The present study attempted to demonstrate that an APTT reagent based on synthetic phospholipids (phosphatidylethanolamine, phosphatidylcholine and phosphatidylserine), which are substances of high purity and homogeneity, reduces batch-to-batch difference when compared with two traditional APTT reagents derived from rabbit brain and soybean. Three types of APTT reagent (SYN-APTT, RBT-APTT, SOY-APTT), prepared respectively from synthetic phospholipid, rabbit brain, and soybean, were tested. The total batch-to-batch difference was coefficient of variation (CV) 0.7-2.4% in the five reagents prepared from synthetic phospholipid (SYN-APTT), but CV 1.5-10.3% in the two traditional reagents (RBT-APTT and SOY-APTT). Additionally, high-performance liquid chromatography (HPLC) analysis showed clear variation in the phospholipid composition of the RBT-APTT and SOY-APTT reagents. In conclusion, the SYN-APTT reagent derived from synthetic phospholipid was shown to reduce batch-to-batch difference, and we therefore suggest that synthetic phospholipid is a substance useful in the preparation of APTT reagent and could contribute to stability of supply and uniform diagnosi…
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The higher the relative content of phosphatidylserine, the less the sensitivity of the assay to LA.
“the higher the relative content of phosphatydilserine, the less the sensitivity of the assay to LA.”
From Lupus Anticoagulant Detection under the Magnifying Glass (2023) · cites this paper as [42]
Cites
Brandt, J. T., Triplett, D. A., Alving, B., & Scharrer, I. (1995) · Thrombosis and Haemostasis · doi:10.1055/s-0038-1649901 · Cited by 993 · see citing works
Kelsey, P. R., Stevenson, K. J., & Poller, L. (1984) · Thrombosis and Haemostasis · doi:10.1055/s-0038-1661166 · Cited by 91 · see citing works
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Lupus Anticoagulant Detection under the Magnifying Glass
Molinari, A. C., Martini, T., Banov, L., Ierardi, A., Leotta, M., Strangio, A., & Santoro, R. C. (2023) · Journal of Clinical Medicine · doi:10.3390/jcm12206654 · Cited by 9 · see citing works
“In addition, the representativeness of the different phospholipids classes can influence the assay performance [42–44]: the higher the relative content of phosphatydilserine, the less the sensitivity of the assay to LA.”
BackgroundAnalytical dilemmas in lupus anticoagulant detection
Moore, G. W. (2023) · Exploration of Immunology · doi:10.37349/ei.2023.00104 · Cited by 5 · see citing works
- Authors
- Okuda, M., & Yamamoto, Y.
- Affiliations
- Sysmex (Japan) (JP)
- Corresponding author
- Okuda, M.
- ORCID
- Yamamoto, Y.
- Venue
- Clinical and Laboratory Haematology
- Publisher
- Wiley
- Published
- 2004-06
- Volume
- 26
- Issue
- 3
- Pages
- 215-223
- ISSN
- 0141-9854 (ISSN-L)
- 1365-2257
- OpenAlex
- W2136551605
- Cited by
- 47
- References
- 2
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 7 · 2023: 7 · 2022: 4 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 2 · 2017: 2 · 2016: 1 · 2014: 3 · 2013: 2 · 2012: 2
- FWCI
- 0.64
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Clinical & Laboratory Haematology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Hematology › Blood Coagulation and Thrombosis Mechanisms
- Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Blood properties and coagulation
- Keywords
- activated partial thromboplastin time, synthetic phospholipids, batch-to-batch variation, phosphatidylethanolamine, phosphatidylcholine, phosphatidylserine, soybean phospholipids, reagent stability
- MeSH terms
- Animals, Heparin, Humans, Indicators and Reagents, Partial Thromboplastin Time, Phospholipids, Reproducibility of Results, Lupus Coagulation Inhibitor, Drug Monitoring
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