The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Use of the polymerase chain reaction for direct detection of Listeria monocytogenes in soft cheese
Abstract
The polymerase chain reaction (PCR) amplification technique was investigated as a tool for direct detection of Listeria monocytogenes in soft cheeses. Different sets of oligonucleotide primers were used, and parts of the L. monocytogenes Dth18‐gene could be amplified specifically when either a plasmid vector carrying the cloned gene or chromosomal DNA was used as a template. The detection limit for L. monocytogenes in dilutions of pure cultures was between 1 and 10 colony‐forming units. In extracts from soft cheeses containing L. monocytogenes DNA, the amplification was strongly inhibited. This inhibition could be reduced by an additional purification step. Despite this the detection limit showed a large variation, depending on the brand of cheese used. In some cheeses 10 3 cfu/0.5 g could be visualized whereas in others the presence of 10 8 cfu/0.5 g did not yield a detectable quantity of amplified product.
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Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Cited by
Microbiological Safety of Drinking Water
Szewzyk, U., Szewzyk, R., Manz, W., & Schleifer, K.-H. (2000) · Annual Review of Microbiology · doi:10.1146/annurev.micro.54.1.81 · Cited by 580
Methods for the isolation and identification of Listeria spp. and Listeria monocytogenes: a review
Gasanov, U., Hughes, D., & Hansbro, P. M. (2005) · FEMS Microbiology Reviews · doi:10.1016/j.femsre.2004.12.002 · Cited by 410
Metadata
- Authors
- Wernars, K., Heuvelman, C. J., Chakraborty, T., & Notermans, S. H. W.
- Venue
- Journal of Applied Bacteriology
- Publisher
- Wiley
- Published
- 1991-02
- Volume
- 70
- Issue
- 2
- Pages
- 121-126
- ISSN
- 0021-8847 (ISSN-L)
- OpenAlex
- W2057519138
- Cited by
- 200
- References
- 1
- FWCI
- 20.30
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Use of the polymerase chain reaction for direct detection of Listeria monocytogenes in soft cheese. https://app.cochat.ai/papers/10.1111/j.1365-2672.1991.tb04437.x
- The paper
- Wernars, K., Heuvelman, C. J., Chakraborty, T., & Notermans, S. H. W. (1991). Use of the polymerase chain reaction for direct detection of Listeria monocytogenes in soft cheese. Journal of Applied Bacteriology. https://doi.org/10.1111/j.1365-2672.1991.tb04437.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
