The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
Rapid assessment of bacterial viability and vitality by rhodamine 123 and flow cytometry
Abstract
A.S. KAPRELYANTS AND D.B. KELL. 1992. The fluorescent dye rhodamine 123 (Rh 123) is concentrated by microbial cells in an uncoupler‐sensitive fashion. Steady‐state fluorescence measurements with Micrococcus luteus indicated that provided the added dye concentration is below approximately 1 mmol/1, uptake is fully uncoupler‐sensitive and is not accompanied by significant self‐quenching of the fluorescence of accumulated dye molecules. ‘Viable’ and ‘non‐viable’ cells are easily and quantitatively distinguished in a flow cytometer by the extent to which they accumulate the dye. The viability of a very slowly growing chemostat culture of Mic. luteus is apparently only about40–50%, as judged by plate counts, but most of the ‘non‐viable’ cells can be resuscitated by incubation of the culture in nutrient medium before plating. The extent to which individual cells accumulate rhodamine 123 can be rapidly assessed by flow cytometry, and reflects the three distinguishable physiological states exhibited by the culture (‘non‐viable’, ‘viable’ and ‘non‐viable‐but‐resuscitable’). Gram‐negative bacteria do not accumulate rhodamine 123 significantly because their outer membrane is not permeable to it; a simple treatment overcomes this. Flow cytometry using rhodamine 123 should prove of general utility for the rapid assessment of microbial viability and vitality.
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Cited by
Boulos, L., Prévost, M., Barbeau, B., Coallier, J., & Desjardins, R. (1999) · Journal of Microbiological Methods · doi:10.1016/s0167-7012(99)00048-2 · Cited by 1,125
Bacterial viability and antibiotic susceptibility testing with SYTOX green nucleic acid stain
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Mukamolova, G. V., Kaprelyants, A. S., Young, D. I., Young, M., & Kell, D. B. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.15.8916 · Cited by 448
Self-citationApplications of flow cytometry to clinical microbiology
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Trends in rhizobial inoculant production and use
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Metadata
- Authors
- Kaprelyants, A. S., & Kell, D. B.
- Venue
- Journal of Applied Bacteriology
- Publisher
- Wiley
- Published
- 1992-05
- Volume
- 72
- Issue
- 5
- Pages
- 410-422
- ISSN
- 0021-8847 (ISSN-L)
- OpenAlex
- W1964812394
- Cited by
- 269
- References
- 35
- FWCI
- 10.14
- Type
- journal article
- Language
- en
- Open access
- closed
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- Kaprelyants, A. S., & Kell, D. B. (1992). Rapid assessment of bacterial viability and vitality by rhodamine 123 and flow cytometry. Journal of Applied Bacteriology. https://doi.org/10.1111/j.1365-2672.1992.tb01854.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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