The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Bacterial viability and antibiotic susceptibility testing with SYTOX green nucleic acid stain
Open accessAbstract
A fluorescent nucleic acid stain that does not penetrate living cells was used to assess the integrity of the plasma membranes of bacteria. SYTOX Green nucleic acid stain is an unsymmetrical cyanine dye with three positive charges that is completely excluded from live eukaryotic and prokaryotic cells. Binding of SYTOX Green stain to nucleic acids resulted in a > 500-fold enhancement in fluorescence emission (absorption and emission maxima at 502 and 523 nm, respectively), rendering bacteria with compromised plasma membranes brightly green fluorescent. SYTOX Green stain is readily excited by the 488-nm line of the argon ion laser. The fluorescence signal from membrane-compromised bacteria labeled with SYTOX Green stain was typically > 10-fold brighter than that from intact organisms. Bacterial suspensions labeled with SYTOX Green stain emitted green fluorescence in proportion to the fraction of permeabilized cells in the population, which was quantified by microscopy, fluorometry, or flow cytometry. Flow cytometric and fluorometric approaches were used to quantify the effect of beta-lactam antibiotics on the cell membrane integrity of Escherichia coli. Detection and discrimination of live and permeabilized cells labeled with SYTOX Green stain by flow cytometry were markedly improved over those by propidium iodide-based tests. These studies showed that bacterial labeling with SYTOX Green stain is an effective alternative to conventional methods for measuring bacterial viability and antibiotic susceptibility.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Manual of Clinical Microbiology.
(1980) · Annals of Internal Medicine · doi:10.7326/0003-4819-93-6-939_2 · Cited by 14,411
Shapiro, H. M. (2003) · Wiley eBooks · doi:10.1002/0471722731 · Cited by 2,671
Kepner, R. L., & Pratt, J. R. (1994) · Microbiological Reviews · doi:10.1128/mmbr.58.4.603-615.1994 · Cited by 797
Papadopoulos, N. G., Dedoussis, G. V. Z., Spanakos, G., Gritzapis, A. D., Baxevanis, C. N., & Papamichail, M. (1994) · Journal of Immunological Methods · doi:10.1016/0022-1759(94)90147-3 · Cited by 288
Dual DNA Staining Assessment of Bovine Sperm Viability Using SYBR‐14 and Propidium Iodide
GARNER, D. L., JOHNSON, L. A., YUE, S. T., ROTH, B. L., & HAUGLAND, R. P. (1994) · Journal of Andrology · doi:10.1002/j.1939-4640.1994.tb00510.x · Cited by 285
Self-citationFluorogenic and chromogenic substrates used in bacterial diagnostics
Manafi, M., Kneifel, W., & Bascomb, S. (1991) · Microbiological Reviews · doi:10.1128/mmbr.55.3.335-348.1991 · Cited by 277
Rapid assessment of bacterial viability and vitality by rhodamine 123 and flow cytometry
Kaprelyants, A. S., & Kell, D. B. (1992) · Journal of Applied Bacteriology · doi:10.1111/j.1365-2672.1992.tb01854.x · Cited by 269
Schaule, G., Flemming, H. C., & Ridgway, H. F. (1993) · Applied and Environmental Microbiology · doi:10.1128/aem.59.11.3850-3857.1993 · Cited by 199
Development of a robust flow cytometric assay for determining numbers of viable bacteria
Jepras, R. I., Carter, J., Pearson, S. C., Paul, F. E., & Wilkinson, M. J. (1995) · Applied and Environmental Microbiology · doi:10.1128/aem.61.7.2696-2701.1995 · Cited by 185
Physiological assessment of bacteria using fluorochromes
McFeters, G. A., Yu, F. P., Pyle, B. H., & Stewart, P. S. (1995) · Journal of Microbiological Methods · doi:10.1016/0167-7012(94)00027-5 · Cited by 158
Cited by
Pilsczek, F. H., Salina, D., Poon, K. K. H., Fahey, C., Yipp, B. G., Sibley, C. D., Robbins, S. M., Green, F. H. Y., Surette, M. G., Sugai, M., et al. (2010) · The Journal of Immunology · doi:10.4049/jimmunol.1000675 · Cited by 1,114
Peptidomimetic Antibiotics Target Outer-Membrane Biogenesis in Pseudomonas aeruginosa
Srinivas, N., Jetter, P., Ueberbacher, B. J., Werneburg, M., Zerbe, K., Steinmann, J., Van der Meijden, B., Bernardini, F., Lederer, A., Dias, R. L. A., et al. (2010) · Science · doi:10.1126/science.1182749 · Cited by 554
Fatty acid production in genetically modified cyanobacteria
Liu, X., Sheng, J., & Curtiss III, R. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1103014108 · Cited by 532
Choi, U., & Lee, C.-R. (2019) · Frontiers in Microbiology · doi:10.3389/fmicb.2019.00953 · Cited by 382
Mode-of-Action of Antimicrobial Peptides: Membrane Disruption vs. Intracellular Mechanisms
Benfield, A. H., & Henriques, S. T. (2020) · Frontiers in Medical Technology · doi:10.3389/fmedt.2020.610997 · Cited by 367
Metadata
- Authors
- Roth, B. L., Poot, M., Yue, S. T., & Millard, P. J.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1997-06
- Volume
- 63
- Issue
- 6
- Pages
- 2421-2431
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2143587535
- Cited by
- 579
- References
- 30
- FWCI
- 6.59
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Bacterial viability and antibiotic susceptibility testing with SYTOX green nucleic acid stain. https://app.cochat.ai/papers/10.1128/aem.63.6.2421-2431.1997
- The paper
- Roth, B. L., Poot, M., Yue, S. T., & Millard, P. J. (1997). Bacterial viability and antibiotic susceptibility testing with SYTOX green nucleic acid stain. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.63.6.2421-2431.1997
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
