The DOI matches its Crossref record; 55 of the 55 references it cites were found in a registry or an index.
Suppression of bacterial cell–cell signalling, biofilm formation and type III secretion system by citrus flavonoids
Abstract
AIM: This study investigated the quorum sensing, biofilm and type three secretion system (TTSS) inhibitory properties of citrus flavonoids. METHODS AND RESULTS: Flavonoids were tested for their ability to inhibit quorum sensing using Vibrio harveyi reporter assay. Biofilm assays were carried out in 96-well plates. Inhibition of biofilm formation in Escherichia coli O157:H7 and V. harveyi by citrus flavonoids was measured. Furthermore, effect of naringenin on expression of V. harveyi TTSS was investigated by semi-quantitative PCR. Differential responses for different flavonoids were observed for different cell-cell signalling systems. Among the tested flavonoids, naringenin, kaempferol, quercetin and apigenin were effective antagonists of cell-cell signalling. Furthermore, these flavonoids suppressed the biofilm formation in V. harveyi and E. coli O157:H7. In addition, naringenin altered the expression of genes encoding TTSS in V. harveyi. CONCLUSION: The results of the study indicate a potential modulation of bacterial cell-cell communication, E. coli O157:H7 biofilm and V. harveyi virulence, by flavonoids especially naringenin, quercetin, sinensetin and apigenin. Among the tested flavonoids, naringenin emerged as potent and possibly a nonspecific inhibitor of autoinducer-mediated cell-cell signalling. Naringenin and other flavonoids are prominent secondary metabolites present in citrus species. Therefore, citrus, being a major source of some of these flavonoids and by virtue of widely consumed fruit, may modulate the intestinal microflora. SIGNIFICANCE AND IMPACT OF THE STUDY: Currently, a limited number of naturally occurring compounds have demonstrated their potential in inhibition of cell-cell communications; therefore, citrus flavonoids may be useful as lead compounds for the development of antipathogenic agents.
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Cited by
Polyphenol-Mediated Gut Microbiota Modulation: Toward Prebiotics and Further
Rodríguez-Daza, M. C., Pulido-Mateos, E. C., Lupien-Meilleur, J., Guyonnet, D., Desjardins, Y., & Roy, D. (2021) · Frontiers in Nutrition · doi:10.3389/fnut.2021.689456 · Cited by 474
Prevention of biofilm formation by quorum quenching
Paluch, E., Rewak-Soroczyńska, J., Jędrusik, I., Mazurkiewicz, E., & Jermakow, K. (2020) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-020-10349-w · Cited by 445
Small molecule control of bacterial biofilms
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Angusamy, A., Balasubramanian, V., Arunmurugan, B., Arunachalam, K., Issac Abraham, S. V. P., Murugesan, S., Krishnasamy, B., Sundaram, J., & Arumugam, V. R. (2023) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-023-03578-4 · Cited by 10
Metadata
- Authors
- Vikram, A., Jayaprakasha, G. K., Jesudhasan, P. R., Pillai, S. D., & Patil, B. S.
- Venue
- Journal of Applied Microbiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2010-01-19
- Volume
- 109
- Issue
- 2
- Pages
- 515-527
- ISSN
- 1364-5072 (ISSN-L)
- 1365-2672
- OpenAlex
- W1992034132
- Cited by
- 393
- References
- 55
- FWCI
- 4.84
- Type
- journal article
- Language
- en
- Open access
- closed
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- Vikram, A., Jayaprakasha, G. K., Jesudhasan, P. R., Pillai, S. D., & Patil, B. S. (2010). Suppression of bacterial cell–cell signalling, biofilm formation and type III secretion system by citrus flavonoids. Journal of Applied Microbiology. https://doi.org/10.1111/j.1365-2672.2010.04677.x
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