The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
Resin development for electron microscopy and an analysis of embedding at low temperature*
Abstract
SUMMARY: We have tested a wide range of acrylate and methacrylate resin formulations and have concluded that embedding resins can be developed which are usable within a broad range of environmental conditions. To demonstrate this versatility, we have designed two highly‐cross‐linked resins, one polar and the other nonpolar, that are usable to temperatures from 243 to 223 K. Both of these resins formulations, which are now commercially available, show that systematic experiments can be easily done to study the effects of environmental parameters, such as water content, temperature, or resin polarity, on biological material during embedding. Using these resins and aldehyde‐fixed protein crystals, it can be shown that low temperature minimizes the loss of molecular structure to an extent that is not often obtainable with conventional methods of dehydration and embedding. Embedded crystals of aspartate aminotransferase still retained molecular order to 0·6 nm. Embedded crystals of catalase show X‐ray diffraction maxima out to 0·8 nm. When sectioned, catalase revealed stain‐limited electron and optical diffraction patterns to 2·5 nm. Nevertheless, our work clearly demonstrates that low temperature embedding procedures are superior and that versatile, general purpose resins can be designed to take advantage of this fact.
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Citations
Cites
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Self-citationCrystallographic studies of the dynamic properties of lysozyme
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A MACROMOLECULAR REPEATING UNIT OF MITOCHONDRIAL STRUCTURE AND FUNCTION
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Cited by
Roth, J. (1985) · Cell · doi:10.1016/0092-8674(85)90034-0 · Cited by 453
Roth, J. (1983) · Journal of Histochemistry & Cytochemistry · doi:10.1177/31.8.6190857 · Cited by 381
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Carlsson, S. R., Roth, J., Piller, F., & Fukuda, M. (1988) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)37369-1 · Cited by 345
The cell wall architecture of Candida albicans wild‐type cells and cell wall‐defective mutants
Kapteyn, J. C., Hoyer, L. L., Hecht, J. E., Müller, W. H., Andel, A., Verkleij, A. J., Makarow, M., Van Den Ende, H., & Klis, F. M. (2000) · Molecular Microbiology · doi:10.1046/j.1365-2958.2000.01729.x · Cited by 328
Metadata
- Authors
- Carlemalm, E., Garavito, R. M., & Villiger, W.
- Venue
- Journal of Microscopy
- Publisher
- Wiley
- Published
- 1982-05
- Volume
- 126
- Issue
- 2
- Pages
- 123-143
- ISSN
- 0022-2720 (ISSN-L)
- 1365-2818
- OpenAlex
- W2018778009
- Cited by
- 707
- References
- 14
- FWCI
- 24.02
- Type
- journal article
- Language
- en
- Open access
- closed
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- Carlemalm, E., Garavito, R. M., & Villiger, W. (1982). Resin development for electron microscopy and an analysis of embedding at low temperature*. Journal of Microscopy. https://doi.org/10.1111/j.1365-2818.1982.tb00362.x
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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