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Orthogonal‐plane fluorescence optical sectioning: Three‐dimensional imaging of macroscopic biological specimens
Abstract
Summary: An imaging technique called orthogonal‐plane fluorescence optical sectioning (OPFOS) was developed to image the internal architecture of the cochlea. Expressions for the three‐dimensional point spread function and the axial and lateral resolution are derived. Methodologies for tissue preparation and for construction, alignment, calibration and characterization of an OPFOS apparatus are presented. The instrument described produced focused, high‐resolution images of optical sections of an intact, excised guineapig cochlea. The lateral and axial resolutions of the images were 10 and 26 μm, respectively, within a 1·5‐mm field of view.
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Bojarczuk, A., Miller, K. A., Hotham, R., Lewis, A., Ogryzko, N. V., Kamuyango, A. A., Frost, H., Gibson, R. H., Stillman, E., May, R. C., et al. (2016) · Scientific Reports · doi:10.1038/srep21489 · Cited by 170
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Metadata
- Authors
- VOIE, A. H., BURNS, D. H., & SPELMAN, F. A.
- Venue
- Journal of Microscopy
- Publisher
- Wiley
- Published
- 1993-06
- Volume
- 170
- Issue
- 3
- Pages
- 229-236
- ISSN
- 0022-2720 (ISSN-L)
- 1365-2818
- OpenAlex
- W2040870938
- Cited by
- 477
- References
- 0
- FWCI
- 1.41
- Type
- journal article
- Language
- en
- Open access
- closed
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- VOIE, A. H., BURNS, D. H., & SPELMAN, F. A. (1993). Orthogonal‐plane fluorescence optical sectioning: Three‐dimensional imaging of macroscopic biological specimens. Journal of Microscopy. https://doi.org/10.1111/j.1365-2818.1993.tb03346.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
