The DOI matches its Crossref record.
3‐D PSF fitting for fluorescence microscopy: implementation and localization application
Open accessAbstract
Summary: Localization microscopy relies on computationally efficient Gaussian approximations of the point spread function for the calculation of fluorophore positions. Theoretical predictions show that under specific experimental conditions, localization accuracy is significantly improved when the localization is performed using a more realistic model. Here, we show how this can be achieved by considering three‐dimensional (3‐D) point spread function models for the wide field microscope. We introduce a least‐squares point spread function fitting framework that utilizes the Gibson and Lanni model and propose a computationally efficient way for evaluating its derivative functions. We demonstrate the usefulness of the proposed approach with algorithms for particle localization and defocus estimation, both implemented as plugins for ImageJ.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Quantitative evaluation of software packages for single-molecule localization microscopy
Sage, D., Kirshner, H., Pengo, T., Stuurman, N., Min, J., Manley, S., & Unser, M. (2015) · Nature Methods · doi:10.1038/nmeth.3442 · Cited by 396
Self-citationSvahn, A. J., Don, E. K., Badrock, A. P., Cole, N. J., Graeber, M. B., Yerbury, J. J., Chung, R., & Morsch, M. (2018) · Acta Neuropathologica · doi:10.1007/s00401-018-1875-2 · Cited by 87
Mitochondrial origins of the pressure to sleep
Sarnataro, R., Velasco, C. D., Monaco, N., Kempf, A., & Miesenböck, G. (2025) · Nature · doi:10.1038/s41586-025-09261-y · Cited by 62
Jelli, E., Ohmura, T., Netter, N., Abt, M., Jiménez‐Siebert, E., Neuhaus, K., Rode, D. K. H., Nadell, C. D., & Drescher, K. (2023) · Molecular Microbiology · doi:10.1111/mmi.15064 · Cited by 48
Synthetic Par polarity induces cytoskeleton asymmetry in unpolarized mammalian cells
Watson, J. L., Krüger, L. K., Ben-Sasson, A. J., Bittleston, A., Shahbazi, M. N., Planelles-Herrero, V. J., Chambers, J. E., Manton, J. D., Baker, D., & Derivery, E. (2023) · Cell · doi:10.1016/j.cell.2023.08.034 · Cited by 32
Metadata
- Authors
- KIRSHNER, H., AGUET, F., SAGE, D., & UNSER, M.
- Venue
- Journal of Microscopy
- Publisher
- Wiley
- Published
- 2012-11-05
- Volume
- 249
- Issue
- 1
- Pages
- 13-25
- ISSN
- 0022-2720 (ISSN-L)
- 1365-2818
- OpenAlex
- W2120280503
- Cited by
- 277
- References
- 0
- FWCI
- 4.36
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: 3‐D PSF fitting for fluorescence microscopy: implementation and localization application. https://app.cochat.ai/papers/10.1111/j.1365-2818.2012.03675.x
- The paper
- KIRSHNER, H., AGUET, F., SAGE, D., & UNSER, M. (2013). 3‐D PSF fitting for fluorescence microscopy: implementation and localization application. Journal of Microscopy. https://doi.org/10.1111/j.1365-2818.2012.03675.x
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
