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The calmodulin‐sensitive adenylate cyclase of Bordetella pertussis: cloning and expression in Escherichia col
Abstract
Summary: The adenylate cyclase toxin of the prokaryote Bordetella pertussis is stimulated by the eukaryotic regulatory protein, calmodulin. A general strategy, using the adenylate‐cyclase‐calmodulin interaction as a tool, has permitted cloning and expression of the toxin in Escherichia coli in the absence of any B. pertussis trans‐activating factor. We show that the protein is synthesized in a large precursor form composed of 1706 amino acids. The calmodulin‐stimulated catalytic activity resides in the amino‐terminal 450 amino acids of the adenylate cyclase. The enzyme expressed in E. coli is recognized in Western blots by antibodies directed against purified B. pertussis adenylate cyclase, and its activity is inhibited by these antibodies.
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Citations
Cited by
Botsford, J. L., & Harman, J. G. (1992) · Microbiological Reviews · doi:10.1128/mr.56.1.100-122.1992 · Cited by 558
RTX proteins: a highly diverse family secreted by a common mechanism
Linhartová, I., Bumba, L., Mašín, J., Basler, M., Osička, R., Kamanová, J., Procházková, K., Adkins, I., Hejnová-Holubová, J., Sadílková, L., et al. (2010) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2010.00231.x · Cited by 530
Translocation of a hybrid YopE‐adenylate cyclase from Yersinia enterocolitica into HeLa cells
Sory, M., & Cornelis, G. R. (1994) · Molecular Microbiology · doi:10.1111/j.1365-2958.1994.tb02191.x · Cited by 532
Molecular characterization of the Clostridium difficile toxin A gene
Dove, C. H., Wang, S. Z., Price, S. B., Phelps, C. J., Lyerly, D. M., Wilkins, T. D., & Johnson, J. L. (1990) · Infection and Immunity · doi:10.1128/iai.58.2.480-488.1990 · Cited by 269
Castets, F., Bartoli, M., Barnier, J. V., Baillat, G., Salin, P., Moqrich, A., Bourgeois, J. P., Denizot, F., Rougon, G., Calothy, G., et al. (1996) · The Journal of Cell Biology · doi:10.1083/jcb.134.4.1051 · Cited by 129
Metadata
- Authors
- Glaser, P., Ladant, D., Sezer, O., Pichot, F., Ullmann, A., & Danchin, A.
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 1988-01
- Volume
- 2
- Issue
- 1
- Pages
- 19-30
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- OpenAlex
- W2048895914
- Cited by
- 313
- References
- 0
- FWCI
- 28.29
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: The calmodulin‐sensitive adenylate cyclase of Bordetella pertussis: cloning and expression in Escherichia col. https://app.cochat.ai/papers/10.1111/j.1365-2958.1988.tb00003.x
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- Glaser, P., Ladant, D., Sezer, O., Pichot, F., Ullmann, A., & Danchin, A. (1988). The calmodulin‐sensitive adenylate cyclase of Bordetella pertussis: cloning and expression in Escherichia col. Molecular Microbiology. https://doi.org/10.1111/j.1365-2958.1988.tb00003.x
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