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Cited by 269

The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.

Molecular characterization of the Clostridium difficile toxin A gene

Open access
Dove, C H, Wang, S Z, Price, S B, Phelps, C J, Lyerly, D M, Wilkins, T D, Johnson, J L·1990·Infection and Immunity
DOI 10.1128/iai.58.2.480-488.1990
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Abstract

The gene encoding the toxin A protein of Clostridium difficile (strain VPI 10463) was cloned and sequenced. The coding region of 8,133 base pairs had a mol% G + C of 26.9 and encodes 2,710 amino acids. The deduced polypeptide has a molecular mass of ca. 308 kilodaltons. Nearly a third of the gene, at the 3' end, consists of 38 repeating sequences. The repeating units were grouped into two classes, I and II, on the basis of length and the low levels of DNA sequence similarities between them. There were seven class I repeating units, each containing 90 nucleotides, and 31 class II units, which, with two exceptions, were either 60 or 63 nucleotides in length. On the basis of DNA sequence similarities, the class II repeating units were further segregated into subclasses: 7 class IIA, 13 class IIB, 5 class IIC, and 6 class IID. The dipeptide tyrosine-phenylalanine was found in all 38 repeating units, and other amino acid sequences were unique to a specific class or subclass. This region of the protein has epitopes for the monoclonal antibody PCG-4 and includes the binding region for the Gal alpha 1-3Gal beta 1-4GlcNAc carbohydrate receptor. Located 1,350 base pairs upstream from the toxin A translation start site is the 3' end of the toxin B gene. Between the two toxin genes is a small open reading frame, which encodes a deduced polypeptide of ca. 16 or 19 kilodaltons. The role of this open reading frame is unknown.

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Metadata

Authors
Dove, C. H., Wang, S. Z., Price, S. B., Phelps, C. J., Lyerly, D. M., Wilkins, T. D., & Johnson, J. L.
Venue
Infection and Immunity
Publisher
American Society for Microbiology
Published
1990-02
Volume
58
Issue
2
Pages
480-488
ISSN
  • 0019-9567 (ISSN-L)
  • 1098-5522
DOI
10.1128/iai.58.2.480-488.1990
OpenAlex
W1906111837
Cited by
269
References
27
FWCI
20.71
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Molecular characterization of the Clostridium difficile toxin A gene. https://app.cochat.ai/papers/10.1128/iai.58.2.480-488.1990
The paper
Dove, C. H., Wang, S. Z., Price, S. B., Phelps, C. J., Lyerly, D. M., Wilkins, T. D., & Johnson, J. L. (1990). Molecular characterization of the Clostridium difficile toxin A gene. Infection and Immunity. https://doi.org/10.1128/iai.58.2.480-488.1990

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